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pSpCas9n_cofilin sgRNA2 (#RDB20018)

Expression vector of one of the paired sgRNAs for human cofilin and D10A Cas9 nickase mutant.


Drawn by SnapGene® software
Sequence (full) RDB20018hts01.seq
Publication Nakajima, M., FEBS Lett. 597 (8): 1114-1124 (2023). [link to RRC of NBRP]
Test sheet RDB20018_B3Fgp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Expression vector of one of the paired sgRNAs for human cofilin and D10A Cas9 nickase mutant. See Fig.3 of Nakajima, M. et al., FEBS Lett. 597 (8): 1114-1124 (2023).
Vector backbone pSpCas9n(BB)-2A-Puro (PX462) V2.0 (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cells)
Growth conditions LB+Amp, 37oC
Gene/insert name human CFL1 oligo DNA.
Depositor|Developer Simizu, Siro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Nakajima, M. et al. FEBS Lett. 597(8): 1114-1124, 2023)
Additional terms and conditions:
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用すること(Nakajima, M. et al. FEBS Lett. 597(8): 1114-1124, 2023)。
付加的使用条件：
このリソースは、非営利機関の非営利学術研究に限って提供する。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20018 pSpCas9n_cofilin sgRNA2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pSpCas9n_cofilin sgRNA2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20018).

Reference section:

Nakajima, M., Kawahara, R., Simizu, S., Cofilin promotes vasculogenic mimicry by regulating the actin cytoskeleton in human breast cancer cells. FEBS Lett. 597 (8): 1114-1124 (2023). PMID 36737242. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nakajima, M., Cofilin promotes vasculogenic mimicry by regulating the actin cytoskeleton in human breast cancer cells. FEBS Lett. 597 (8): 1114-1124 (2023). PMID 36737242. [link to RRC of NBRP]