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pCI-neo MFAP4-HA WF (#RDB18692)

Expression vector of human MFAP4, mutant W235F.

Clone info. Expression vector of human Microfibril-associated glycoprotein 4 (MFAP4) mutant W235F tagged with 3×HA at C-terminus, Fig 4 of Osada, Y. et al., Biochim. Biophys. Acta Gen. Subj., 1864 (9): 129637, 2020.
Comment The MFAP4 cDNA was from the IRAK141E15 (HGX056511). Expression was confirmed by the depositor with western blotting.
Vector backbone pCI-neo (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Growth conditions LB+Ampicillin, 37oC
Gene/insert name human MFAP4 cDNA
Depositor|Developer Simizu, Siro |
 
Sequence (full) RDB18692hts01.seq checkAssembled from experimentally sequenced data.
Publication Osada, Y., Biochim. Biophys. Acta. Gen. Subj. 1864 (9): 129637 (2020). [link to RRC of NBRP]
Test sheet RDB18692C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Osada, Y. et al., Biochim. Biophys. Acta Gen. Subj., 1864 (9): 129637, 2020).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Osada, Y. et al., Biochim. Biophys. Acta Gen. Subj., 1864 (9): 129637, 2020)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18692 pCI-neo MFAP4-HA WF DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pCI-neo MFAP4-HA WF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18692).

Reference section:

Osada, Y., Suzuki, T., Mizuta, H., Mori, K., Miura, K., Dohmae, N., Simizu, S., The Fibrinogen C-terminal Domain Is Seldom C-mannosylated but Its C-mannosylation Is Important for the Secretion of Microfibril-Associated Glycoprotein 4. Biochim. Biophys. Acta. Gen. Subj. 1864 (9): 129637 (2020). PMID 32442478. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Osada, Y., The Fibrinogen C-terminal Domain Is Seldom C-mannosylated but Its C-mannosylation Is Important for the Secretion of Microfibril-Associated Glycoprotein 4. Biochim. Biophys. Acta. Gen. Subj. 1864 (9): 129637 (2020). PMID 32442478. [link to RRC of NBRP]