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pcDNA3.1/Myc-His(+)-HP/C437S (#RDB14262)

Expression vector of Myc-His tagged human Heparanase (HP) mutant C437S.

Clone info. Expression vector of Myc-His tagged human Heparanase (HP) mutant C437S, see Fig. 1A of Simizu, S., Cancer. Res, 67 (16): 7841-7849 (2007) for details.
Comment Expression was confirmed by the depositor with Western blotting and fluorescent microscopy. cDNAfragment 138nt to 1766nt of NM_006665.5 is cloned. Known differences (at least): substitutions T to C at 419nt (94aa Phe, synonym), A to G at 1,057nt (K307R), A to G at 1,166nt (343aa Glu, synonym).
Vector backbone pcDNA3.1 Myc-His(+) ()
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human HPSE cDNA
Depositor|Developer Simizu, Siro |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Shimizu, S. et al., Cancer Res. 67:7841-7849, 2007)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shimizu, S. et al., Cancer Res. 67:7841-7849, 2007)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14262 pcDNA3.1/Myc-His(+)-HP/C437S DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1/Myc-His(+)-HP/C437S was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14262).

Reference section:

Simizu, S., Suzuki, T., Muroi, M., Lai, N.S., Takagi, S., Dohmae, N., Osada, H., Involvement of disulfide bond formation in the activation of heparanase. Cancer. Res, 67 (16): 7841-7849 (2007). PMID 17699790. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14262_A7B2p1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,T7 pro,insert5'
Sequence file: RDB14262_A7B2a.seq check
>D03620B7_A6Gj_1_CMV-Forward_E11_05_ABI08.ab1
    1 CTTTCCAACT CCCGGCCCCA ATTGACGCAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
  121 ATACGACTCA CTATAGGGAG ACCCAAGCTG GCTAGTTAAG CTTGGTACCG AGCTCGGATC
  181 CACTAGTCCA GTGTGGTGGA ATTCACTAGT GATTATGCTG CTGCGCTCGA AGCCTGCGCT
  241 GCCGCCGCCG CTGATGCTGC TGCTCCTGGG GCCGCTGGGT CCCCTCTCCC CTGGCGCCCT
  301 GCCCCGACCT GCGCAAGCAC AGGACGTCGT GGACCTGGAC TTCTTCACCC AGGAGCCGCT
  361 GCACCTGGTG AGCCCCTCGT TCCTGTCCGT CACCATTGAC GCCAACCTGG CCACGGACCC
  421 GCGGTTCCTC ATCCTCCTGG GTTCTCCAAA GCTTCGTACC TTGGCCAGAG GCTTGTCTCC
  481 TGCGTACCTG AGGTTCGGTG GCACCAAGAC AGACTTCCTA ATTTTCGATC CCAAGAAGGA
  541 ATCAACCTTT GAAGAGAGAA GTTACTGGCA ATCTCAAGTC AACCAGGATA TTTGCAAATA
  601 TGGATCCATC CCTCCTGATG TGGAGGAGAA GTTACGGTTG GAATGGCCCT ACCAGGAGCA
  661 ATTGCTACTC CGAGAACACT ACCAGAAAAA GTTCAAGAAC AGCACCTACT CAAGAAGCTC
  721 TGTAGATGTG CTATACACTT TTGCAAACTG CTCAGGACTG GACTTGATCT TTGGCCTAAA
  781 TGCGTTATTA AGAACAGCAG ATTTGCAGTG GAACAGTTCT AATGCTCAGT TGCTCCTGGA
  841 CTACTGCTCT TCCAAGGGGT ATAACATTTC TTGGGAAACT AGGCAATGAA CCTAACAGTT
  901 TCCTTAAGAA GGCTGATATT TTCATCAATG GGTCGCAGTT AGGGAGAAGA TTTTATTCAA
  961 TTTGCATAAA CTTCTAAGAA AGGTCCACCC TTTCAAAAAT GCAAAACTCT TATGGTTCCC
 1021 TGATGTTGGT CAGCCTCGAA GAAAGGACGG GCTAAGATGG CTGAAGAGCT TCCCTGAACG
1081 CCTGGTGAGA GTTGATTGAA TCAGTACATG GTCATTCAAC T
//
Primer: BGH_rev2 (Pr0606)
Region: bGHp(A),6xHis,Myc,insert3'
Sequence file: RDB14262_A7B2b.seq check
>D03620B7_A6Gj_1_BGH_rev2_F11_06_ABI08.ab1
    1 CATCAAGGGT AGGAGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC
   61 AGTCGAGGCT GATCAGCGGG TTTAAACTCA ATGGTGATGG TGATGATGAC CGGTATGCAT
  121 ATTCAGATCC TCTTCTGAGA TGAGTTTTTG TTCGAATGGG TGACCTCGAG CGGCCGCCAC
  181 TGTGCTGGAT ATCTGCAGAA TTCGATTGAT GCAAGCAGCA ACTTTGGCAT TTCTTATCAC
  241 AAAAAAACTA TATGAGAAAG CTGGCAAGCC CAGTGAACTT CCTGGCCGGA GAGGTTTTTC
  301 CATTAAAGGT GGCAAGGTTT GATCATCCAC CATCTTTAGA GTTAGACCAT TGAGTTGGAC
  361 AGATTTGGAA AGTAATCCAT GAGGTCCCAA AGGTCTTAGA AGGTATTTAT CCACTTGCTT
  421 GTTAGAAAAA GGATAGGGTA ACCGCAAGTA CTTGGTGACA TTATGGAGGT TTATGGCATA
  481 CAGAGTTAAA TCTCCTTCTT TATACCTTGG ATTGTCAGTG TTTGTGCTAT GAAGGTATAC
  541 TCGAAGCTTC CTTCTCTTTG AACCTTGCAC GCTTGCCATT AACACCTTGG TGCCCACCAA
  601 TTTCTTGAAC AGAAGAGATA GCCAATAATC AGGTAAAGGA TCGAAGTTTT CATCCACTAA
  661 ATGGTAGTTT CCTGCTCCAA AGAATACTTG CCTCATCACC ACTTCTATTC CCATTCGGGC
  721 TGACAGGCCC AATTTATCCA GCCACATAAA GCCAGCTGCA AAGGTGTCGG ATAGCAAGGG
  781 CGCTCCGCCT CCATATGCAG AGCTTGTCTC TCCTAACCAG ACCTTCTTGC CAGGCCTGGT
  841 GCTCTCAACC ACCTGGAAAA CTTTTTGCAC AGATGAAATA AAAATGTCCA ATACATCAGG
  901 GTTTAGAAAA TCTTCCCTGG TAGCAGTCCG TCCATTCAAA TAGTAGTGAT GCCATGTAAC
  961 TGAATCAATC ACTTCTCCAC CAGCCTTCAG GAAGCTCTCA GCATCTTAGC CGTCTTTTCT
 1021 TCGAGCTTGA ACGACAATCA GACCCATAGA GGTTTTGGCA ATTTTGGAGT GGACATCTAG
1081 AGTATGCATT GGATAAATCT TTCTCCTACC TTGGC
//
Primer: RDB14252#1 (Pr0404)
Region: insert mid
Sequence file: RDB14262_A7B2c.seq check
>D03620B7_A6Gj_1_RDB14252#1_C11_08_ABI24.ab1
    1 TCTGGAATGG ATGATCTTTG GCCTAATGCG TTATTAAGAA CAGCAGATTT GCAGTGGAAC
   61 AGTTCTAATG CTCAGTTGCT CCTGGACTAC TGCTCTTCCA AGGGGTATAA CATTTCTTGG
  121 GAACTAGGCA ATGAACCTAA CAGTTTCCTT AAGAAGGCTG ATATTTTCAT CAATGGGTCG
  181 CAGTTAGGAG AAGATTTTAT TCAATTGCAT AAACTTCTAA GAAAGTCCAC CTTCAAAAAT
  241 GCAAAACTCT ATGGTCCTGA TGTTGGTCAG CCTCGAAGAA AGACGGCTAA GATGCTGAAG
  301 AGCTTCCTGA AGGCTGGTGG AGAAGTGATT GATTCAGTTA CATGGCATCA CTACTATTTG
  361 AATGGACGGA CTGCTACCAG GGAAGATTTT CTAAACCCTG ATGTATTGGA CATTTTTATT
  421 TCATCTGTGC AAAAAGTTTT CCAGGTGGTT GAGAGCACCA GGCCTGGCAA GAAGGTCTGG
  481 TTAGGAGAGA CAAGCTCTGC ATATGGAGGC GGAGCGCCCT TGCTATCCGA CACCTTTGCA
  541 GCTGGCTTTA TGTGGCTGGA TAAATTGGGC CTGTCAGCCC GAATGGGAAT AGAAGTGGTG
  601 ATGAGGCAAG TATTCTTTGG AGCAGGAAAC TACCATTTAG TGGATGAAAA CTTCGATCCT
  661 TTACCTGATT ATTGGCTATC TCTTCTGTTC AAGAAATTGG TGGGCACCAA GGTGTTAATG
  721 GCAAGCGTGC AAGGTTCAAA GAGAAGGAAG CTTCGAGTAT ACCTTCATAG CACAAACACT
  781 GACAATCCAA GGTATAAAGA AGGAGATTTA ACTCTGTATG CCATAAACCT CCATAATGTC
  841 ACCAAGTACT TGCGGTTACC CTATCCTTTT TCTAACAAGC AAGTGGATAA ATACCTTCTA
  901 AGACCTTTGG GACCTCATGG ATTACTTTCC AAATCTGTCC AACTCAATGG TCTAACTCTA
  961 AAGATGGTGG ATGATCAAAC CCTTGCCACC TTTAATGGAA AAACCCTCTC CGGGCCAGGA
 1021 GTTCACTGGG CTTTGCCCAG CTTTCTCATA TAGTTTTTTT TGTGATAAGA AATGCCAAAG
 1081 AGCTGCTTGC ATCATCGGAA TCTGCAGATA TCAAGCACAG TTGACCACGC TCGGAGGCTC
1141 CACCCCCAAT TTCCGAAC
//
Primer: SV40pro_F (Pr0110)
Region: SV40 pro_ori,NeoR
Sequence file: RDB14262_A7B2d.seq check
>D03620B7_A6Gj_1_SV40pro_F_C08_08_ABI24.ab1
    1 CCCCTGGTCC TGGGGCATTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
   61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA
  121 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
  181 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
  241 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
  301 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
  361 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
  421 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
  481 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
  541 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
  601 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC
  661 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
  721 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC
  781 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT
  841 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
  901 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGCGAAATGA CCGACCAAGC
  961 GACGCCCAAC CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTTCTA TGAAGGTTGG
 1021 GCTTCGGATC GTTTTCCGGA CGCCGGGCTG GATGATCTCA GCGCGGGGGA TCTCATGCCT
 1081 GAGTCTCGCC CACCCCCACT GTTATGGCAG CTATATGTTA CAATTAAAGG CATTAGCAAT
1141 CCACAAATTT CACA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Simizu, S., Involvement of disulfide bond formation in the activation of heparanase. Cancer. Res, 67 (16): 7841-7849 (2007). PMID 17699790. [link to RRC of NBRP]