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pCI-neo/ARL6ip1(1-64)-Gluc-ARL6ip1(65-203) [P64]

Expression vector of human ARL6ip1 plus Gluc inserted after Pro at 64aa.

Catalog number RDB14556
Resource name pCI-neo/ARL6ip1(1-64)-Gluc-ARL6ip1(65-203) [P64]
Clone info. Expression vector of human ADP-ribosylation factor-like 6-interacting protein 1 (ARL6ip1) plus Gluc. Gluc was inserted after Pro at 64aa. The structure of the plasmid construct, see Fig. 1B and C of Kuroda, M. et al. FEBS Lett., 587 (22): 3656-3660, 2013.
Expression was confirmed by the depositor with redox-sensitive luciferase assay.
Vector backbone pCI-neo (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human ARL6IP1 cDNA Gaussia princeps Luciferase cDNA
Depositor Simizu, Siro |
 
Other clones in our bank human ARL6IP1 (NCBI Gene 23204) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Masahiro, K. et al. FEBS Lett. 587, 3656-3660, 2013)
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14556 pCI-neo/ARL6ip1(1-64)-Gluc-ARL6ip1(65-203) [P64] Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Kuroda, M., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Kuroda, M., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029.

2019.07.19

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