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lentiGuidePuro-gRNA2 (#RDB18688)

Nickase knocking out vector of human CEACAM1, gRNA2.

Clone info. Nickase knocking out vector of human carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1), Fig.3 of Hayashi, S. et al., Biochem. Biophys. Rep., 21: 100734, 2020. For targeting exon2 of the CEACAM1, use with lentiGuidePuro-gRNA1.
Vector backbone lentiGuidePuro (plasmid)
Size of vector backbone 10.2 kb
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Growth conditions LB+Ampicillin, 37oC
Growth remarks Use Stbl3 or equivalent to amplify this clone.
Gene/insert name human CEACAM1 cDNA
Depositor|Developer Simizu, Siro |
 
Sequence (full) RDB18688hts01.seq checkAssembled from experimentally sequenced data.
Publication Hayashi, S., Biochem. Biophys. Rep. 21: 100734 (2020). [link to RRC of NBRP]
Test sheet RDB18688C0p1-1.pdf 
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Hayashi, S. et al., Biochem. Biophys. Rep., 21: 100734, 2020).
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hayashi, S. et al., Biochem. Biophys. Rep., 21: 100734, 2020)。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18688 lentiGuidePuro-gRNA2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The lentiGuidePuro-gRNA2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18688).

Reference section:

Hayashi, S., Osada, Y., Miura, K., Simizu, S., Cell-dependent regulation of vasculogenic mimicry by carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1). Biochem. Biophys. Rep. 21: 100734 (2020). PMID 32025578. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hayashi, S., Cell-dependent regulation of vasculogenic mimicry by carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1). Biochem. Biophys. Rep. 21: 100734 (2020). PMID 32025578. [link to RRC of NBRP]