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pCI-neo-FGL1(K65R)-MH (#RDB19547)

Expression vector of human FGL1 K65R mutant tagged with c-Myc and His6 at C-terminus.

Clone info. Expression vector of human fibrinogen like 1 (FGL1) K65R mutant tagged with c-Myc and His6 at C-terminus. See Fig.1-4 of Kento. M. et al. Biochem. Biophys. Res. Commun. 560: 93-98, 2021.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pCI-neo (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name human FGL1 cDNA
Depositor|Developer Simizu, Siro |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kento. M. et al., Biochem. Biophys. Res. Commun. 560: 93-98, 2021)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kento. M. et al., Biochem. Biophys. Res. Commun. 560: 93-98, 2021)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19547 pCI-neo-FGL1(K65R)-MH Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pCI-neo-FGL1(K65R)-MH was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19547).

Reference section:

Mori, K., Suzuki, T., Miura, K., Dohmae, N., Simizu, S., Involvement of LH3 and GLT25D1 for glucosyl-galactosyl-hydroxylation on non-collagen-like domain of FGL1. Biochem. Biophys. Res. Commun. 560: 93-98 (2021). PMID 33984770. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Mori, K., Involvement of LH3 and GLT25D1 for glucosyl-galactosyl-hydroxylation on non-collagen-like domain of FGL1. Biochem. Biophys. Res. Commun. 560: 93-98 (2021). PMID 33984770. [link to RRC of NBRP]