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pCI-ECM1

Expression vector of human ECM1 cDNA

Catalog number RDB13302
Resource name pCI-ECM1
Clone info. Expression vector of human ECM1 cDNA.
Vector backbone pCI-neo (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human ECM1 cDNA
Depositor|Developer Simizu, Siro |
Other clones in our bank

External Database
Human ECM1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(FEBS Open Bio., 4, 879-885 (2014))
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(FEBS Open Bio., 4, 879-885 (2014))
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB13302 pCI-ECM1 DNA solution Please contact us.

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Uematsu, S., N-Glycosylation of extracellular matrix protein 1 (ECM1) regulates its secretion, which is unrelated to lipoid proteinosis. FEBS Open Bio. 4: 879-885 (2014). PMID 25379385.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13302_A5Fnp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB13302_A5Fna.seq check
          >D02166B1-4_A5Fn_CMV-Forward_B11_05.ab1
              1 CTTCACTGCG ATCGCCCGCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT
             61 CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGATCACT AGAAGCTTTA TTGCGGTAGT
            121 TTATCACAGT TAAATTGCTA ACGCAGTCAG TGCTTCTGAC ACAACAGTCT CGAACTTAAG
            181 CTGCAGTGAC TCTCTTAAGG TAGCCTTGCA GAAGTTGGTC GTGAGGCACT GGGCAGGTAA
            241 GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA ACTGGGCTTG TCGAGACAGA
            301 GAAGACTCTT GCGTTTCTGA TAGGCACCTA TTGGTCTTAC TGACATCCAC TTTGCCTTTC
            361 TCTCCACAGG TGTCCACTCC CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ACTTAATACG
            421 ACTCACTATA GGCTAGCCTC GAGATGGGGA CCACAGCCAG AGCAGCCTTG GTCTTGACCT
            481 ATTTGGCTGT TGCTTCTGCT GCCTCTGAGG GAGGCTTCAC GGCTACAGGA CAGAGGCAGC
            541 TGAGGCCAGA GCACTTTCAA GAAGTTGGCT ACGCAGCTCC CCCCTCCCCA CCCCTATCCC
            601 GAAGCCTCCC CATGGATCAC CCTGACTCCT CTCAGCATGG CCCTCCCTTT GAGGGACAGA
            661 GTCAAGTGCA GCCCCCTCCC TCTCAGGAGG CCACCCCTCT CCAACAGGAA AAGCTGCTAC
            721 CTGCCCAACT CCCTGCTGAA AAGGAAGTGG GTCCCCCTCT CCCTCAGGAA GCTGTCCCCC
            781 TCCAAAAAGA GCTGCCCTCT CTCCAGCACC CCAATGAACA GAAAGAAAGG AACGCCAGCT
            841 CCATTTGGGG ACCAGAGCCA TCCAGAACCC TGAGTCCTGG AATGCAGCCC AGCACTGCCC
            901 AACAGGACCC GGTCCCAAGG GGCTGGGGCC ACCGGGCTTG GATGGCTTCC CCCCTGGGCG
            961 GCTTCTCCAG ACATCTGACC AAATCTGGCA TCTAACGTCA GCATGTGGTA TATGTCCCTT
          1021 GACTTACCAC AGTCCAGCTA CTCCAACCTC AATCGCCAGG GTGGAACCTC ATTATGAG
          //
          Primer: ECM1-M_F
          Sequence file: RDB13302_A5Fnb.seq check
          >D02166B1-4_A5Fn_ECM1-M_F_C11_08.ab1
              1 ATAGGGTACC GTCTCCTCAG AGCTGTCCCC CTCCAAAAAG AGCTGCCCTC TCTCCAGCAC
             61 CCCAATGAAC AGAAGGAAGG AACGCCAGCT CCATTTGGGG ACCAGAGCCA TCCAGAACCT
            121 GAGTCCTGGA ATGCAGCCCA GCACTGCCAA CAGGACCGGT CCCAAGGGGG CTGGGGCCAC
            181 CGGCTGGATG GCTTCCCCCC TGGGCGGCCT TCTCCAGACA ATCTGAACCA AATCTGCCTT
            241 CCTAACCGTC AGCATGTGGT ATATGGTCCC TGGAACCTAC CACAGTCCAG CTACTCCCAC
            301 CTCACTCGCC AGGGTGAGAC CCTCAATTTC CTGGAGATTG GATATTCCCG CTGCTGCCAC
            361 TGCCGCAGCC ACACAAACCG CCTAGAGTGT GCCAAACTTG TGTGGGAGGA AGCAATGAGC
            421 CGATTCTGTG AGGCCGAGTT CTCGGTCAAG ACCCGACCCC ACTGGTGCTG CACGCGGCAG
            481 GGGGAGGCTC GGTTCTCCTG CTTCCAGGAG GAAGCTCCCC AGCCACACTA CCAGCTCCGG
            541 GCCTGCCCCA GCCATCAGCC TGATATTTCC TCGGGTCTTG AGCTGCCTTT CCCTCCTGGG
            601 GTGCCCACAT TGGACAATAT CAAGAACATC TGCCACCTGA GGCGCTTCCG CTCTGTGCCA
            661 CGCAACCTGC CAGCTACTGA CCCCCTACAA AGGGAGCTGC TGGCACTGAT CCAGCTGGAG
            721 AGGGAGTTCC AGCGCTGCTG CCGCCAGGGG AACAATCACA CCTGTACATG GAAGGCCTGG
            781 GAGGATACCC TTGACAAATA CTGTGACCGG GAGTATGCTG TGAAGACCCA CCACCACTTG
            841 TGTTGCCGCC ACCCTCCCAG CCCTACTCGG GATGAGTGCT TTGCCCGTCG GGCTCCTTAC
            901 CCCAACTATG ACCGGGGACA TCTTGACCAT TGACATCGGT CGAGTCACCC CCAACCTCAT
            961 GGGCCAACCT CTGTGGAAAC CAAAGAGTTT CTCAACCAAA GCATAAACAT AATTCCTTGG
           1021 GCTGATCCAC AACATGACTG CCCGCTGGCC TGGTGACCTG GCCAATTTCC AGACAGGTCC
          1081 TGCTTGTTGC AAGAGAGGGA AGAAAATTAA AACCCTTTTC
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB13302_A5Fnc.seq check
          >D02166B1-4_A5Fn_pAxCALNL_F1_D11_11.ab1
              1 TAATTAATCT TTATCATGTC TGCTCGAGCA TTAACCCTCA CTAAAGGGAA GCGGCCGCCT
             61 AATGGTGATG GTGATGATGC AGATCCTCTT CTGAGATGAG TTTTTGTTCT TCTTCCTTGG
            121 GCTCAGAGGT GGAGCTGATA TTTGTTCCTC CAGTTGAGCC CTGCTCCCCC TGGCCCTTGG
            181 CGTTCTCAGT GTCTCCAGAC ACTAGAGCCA CGTTCCTCAG ATAATTGATG TTGAAGCAGT
            241 TGACCTGTTC ATCCCCAGGA CTCAGGTAAC AGCAGAGGGC AGGGTCTCGC CAGATGTTAC
            301 GTCGGGGACC ACACAGATCA TTGATGAAGG TTAATTTCTC CTCCTCTGCA CAGCAGGCCT
            361 GTTCTGGAAA TGGCAGGTCA CAGCAGCGGG CAGTCATGTT GTGGATCAGC CCAGGAATAT
            421 GTTTATGCTT GGTGAGAACT CTTTGGTTTC CACAGAGGTG GCCCATGAGG TTGGGGGTGA
            481 CTCGACCGAT GTCAATGGTC AAGATGTCCC GGTCATAGTT GGGGTAAGGA GCCCGACGGG
            541 CAAAGCACTC ATCCCGAGTA GGGCTGGGAG GGTGGCGGCA ACACAAGTGG TGGTGGGTCT
            601 TCACAGCATA CTCCCGGTCA CAGTATTTGT CAAGGGTATC CTCCCAGGCC TTCCATGTAC
            661 AGGTGTGATT GTTCCCCTGG CGGCAGCAGC GCTGGAACTC CCTCTCCAGC TGGATCAGTG
            721 CCAGCAGCTC CCTTTGTAGG GGGTCAGTAG CTGGCAGGTT GCGTGGCACA GAGCGGAAGC
            781 GCCTCAGGTG GCAGATGTTC TTGATATTGT CCAATGTGGG CACCCCAGGA GGGAAAGGCA
            841 GCTCAAGACC CGAGGAAATA TCACGCTGAT GGCTGGGGCA GGCCCGGAGC TGGTAGTGTG
            901 GCTGGGGAGC TTCCTCCTGG AAAGCAGGAG AACCGAGCCC TCCCCCTGCC GCGTGCAGCA
            961 CCCAGTGGGG TCGGGTCTTG ACCGAGAACT CGGCCTCACA GAATCGGCTC ATTGCTTCTC
           1021 CCCACACAAG TTTGGCACAC TCTAGCGGAT TTGTTGGTGG CTGCGGCCAG TGGCCAGCCA
          1081 CCGGGAATTA TCCGAAATCC TTCCCAAAG
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB13302_A5Fnd.seq check
          >D02166B1-4_A5Fn_SV40pro_F_V2_E11_14.ab1
              1 GGAGCCTTTT TTGGAGCTAG GCTTTTGCAA AAGCTTGATT CTTCTGACAC AACAGTCTCG
             61 AACTTAAGGC TAGAGCCACC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG
            121 CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG
            181 CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT
            241 CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG
            301 GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT
            361 TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT
            421 CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG
            481 ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG
            541 ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC
            601 TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC
            661 CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG
            721 TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG
            781 GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA
            841 TCGCCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GGACTCTGGG GTTTCGAAAT
            901 GACCCGACCC AAGCCGACGC CCAAACCTGC CATCACGAAT GGGCCGGCAA TAAAAATATC
            961 CTTTTATTTT CCATAACATC TGGTGGTGTT GGCTTTTTTG GTGGTGATCG GAATAGCGAT
          1021 AGAATTCGCG TATGGTGCCA CTCTCCAGTA CCACTTCTTG ACTCTGGATG GC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Uematsu, S., N-Glycosylation of extracellular matrix protein 1 (ECM1) regulates its secretion, which is unrelated to lipoid proteinosis. FEBS Open Bio. 4: 879-885 (2014). PMID 25379385.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl