Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCI-ECM1

Expression vector of human ECM1 cDNA

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(FEBS Open Bio., 4, 879-885 (2014))
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(FEBS Open Bio., 4, 879-885 (2014))
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

Tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13302_A5Fnp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB13302_A5Fna.seq
>D02166B1-4_A5Fn_CMV-Forward_B11_05.ab1     1 CTTCACTGCG ATCGCCCGCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT    61 CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGATCACT AGAAGCTTTA TTGCGGTAGT   121 TTATCACAGT TAAATTGCTA ACGCAGTCAG TGCTTCTGAC ACAACAGTCT CGAACTTAAG   181 CTGCAGTGAC TCTCTTAAGG TAGCCTTGCA GAAGTTGGTC GTGAGGCACT GGGCAGGTAA   241 GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA ACTGGGCTTG TCGAGACAGA   301 GAAGACTCTT GCGTTTCTGA TAGGCACCTA TTGGTCTTAC TGACATCCAC TTTGCCTTTC   361 TCTCCACAGG TGTCCACTCC CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ACTTAATACG   421 ACTCACTATA GGCTAGCCTC GAGATGGGGA CCACAGCCAG AGCAGCCTTG GTCTTGACCT   481 ATTTGGCTGT TGCTTCTGCT GCCTCTGAGG GAGGCTTCAC GGCTACAGGA CAGAGGCAGC   541 TGAGGCCAGA GCACTTTCAA GAAGTTGGCT ACGCAGCTCC CCCCTCCCCA CCCCTATCCC   601 GAAGCCTCCC CATGGATCAC CCTGACTCCT CTCAGCATGG CCCTCCCTTT GAGGGACAGA   661 GTCAAGTGCA GCCCCCTCCC TCTCAGGAGG CCACCCCTCT CCAACAGGAA AAGCTGCTAC   721 CTGCCCAACT CCCTGCTGAA AAGGAAGTGG GTCCCCCTCT CCCTCAGGAA GCTGTCCCCC   781 TCCAAAAAGA GCTGCCCTCT CTCCAGCACC CCAATGAACA GAAAGAAAGG AACGCCAGCT   841 CCATTTGGGG ACCAGAGCCA TCCAGAACCC TGAGTCCTGG AATGCAGCCC AGCACTGCCC   901 AACAGGACCC GGTCCCAAGG GGCTGGGGCC ACCGGGCTTG GATGGCTTCC CCCCTGGGCG   961 GCTTCTCCAG ACATCTGACC AAATCTGGCA TCTAACGTCA GCATGTGGTA TATGTCCCTT 1021 GACTTACCAC AGTCCAGCTA CTCCAACCTC AATCGCCAGG GTGGAACCTC ATTATGAG //
Primer: ECM1-M_F Sequence file: RDB13302_A5Fnb.seq
>D02166B1-4_A5Fn_ECM1-M_F_C11_08.ab1     1 ATAGGGTACC GTCTCCTCAG AGCTGTCCCC CTCCAAAAAG AGCTGCCCTC TCTCCAGCAC    61 CCCAATGAAC AGAAGGAAGG AACGCCAGCT CCATTTGGGG ACCAGAGCCA TCCAGAACCT   121 GAGTCCTGGA ATGCAGCCCA GCACTGCCAA CAGGACCGGT CCCAAGGGGG CTGGGGCCAC   181 CGGCTGGATG GCTTCCCCCC TGGGCGGCCT TCTCCAGACA ATCTGAACCA AATCTGCCTT   241 CCTAACCGTC AGCATGTGGT ATATGGTCCC TGGAACCTAC CACAGTCCAG CTACTCCCAC   301 CTCACTCGCC AGGGTGAGAC CCTCAATTTC CTGGAGATTG GATATTCCCG CTGCTGCCAC   361 TGCCGCAGCC ACACAAACCG CCTAGAGTGT GCCAAACTTG TGTGGGAGGA AGCAATGAGC   421 CGATTCTGTG AGGCCGAGTT CTCGGTCAAG ACCCGACCCC ACTGGTGCTG CACGCGGCAG   481 GGGGAGGCTC GGTTCTCCTG CTTCCAGGAG GAAGCTCCCC AGCCACACTA CCAGCTCCGG   541 GCCTGCCCCA GCCATCAGCC TGATATTTCC TCGGGTCTTG AGCTGCCTTT CCCTCCTGGG   601 GTGCCCACAT TGGACAATAT CAAGAACATC TGCCACCTGA GGCGCTTCCG CTCTGTGCCA   661 CGCAACCTGC CAGCTACTGA CCCCCTACAA AGGGAGCTGC TGGCACTGAT CCAGCTGGAG   721 AGGGAGTTCC AGCGCTGCTG CCGCCAGGGG AACAATCACA CCTGTACATG GAAGGCCTGG   781 GAGGATACCC TTGACAAATA CTGTGACCGG GAGTATGCTG TGAAGACCCA CCACCACTTG   841 TGTTGCCGCC ACCCTCCCAG CCCTACTCGG GATGAGTGCT TTGCCCGTCG GGCTCCTTAC   901 CCCAACTATG ACCGGGGACA TCTTGACCAT TGACATCGGT CGAGTCACCC CCAACCTCAT   961 GGGCCAACCT CTGTGGAAAC CAAAGAGTTT CTCAACCAAA GCATAAACAT AATTCCTTGG  1021 GCTGATCCAC AACATGACTG CCCGCTGGCC TGGTGACCTG GCCAATTTCC AGACAGGTCC 1081 TGCTTGTTGC AAGAGAGGGA AGAAAATTAA AACCCTTTTC //
Primer: pAxCALNL_F1 Sequence file: RDB13302_A5Fnc.seq
>D02166B1-4_A5Fn_pAxCALNL_F1_D11_11.ab1     1 TAATTAATCT TTATCATGTC TGCTCGAGCA TTAACCCTCA CTAAAGGGAA GCGGCCGCCT    61 AATGGTGATG GTGATGATGC AGATCCTCTT CTGAGATGAG TTTTTGTTCT TCTTCCTTGG   121 GCTCAGAGGT GGAGCTGATA TTTGTTCCTC CAGTTGAGCC CTGCTCCCCC TGGCCCTTGG   181 CGTTCTCAGT GTCTCCAGAC ACTAGAGCCA CGTTCCTCAG ATAATTGATG TTGAAGCAGT   241 TGACCTGTTC ATCCCCAGGA CTCAGGTAAC AGCAGAGGGC AGGGTCTCGC CAGATGTTAC   301 GTCGGGGACC ACACAGATCA TTGATGAAGG TTAATTTCTC CTCCTCTGCA CAGCAGGCCT   361 GTTCTGGAAA TGGCAGGTCA CAGCAGCGGG CAGTCATGTT GTGGATCAGC CCAGGAATAT   421 GTTTATGCTT GGTGAGAACT CTTTGGTTTC CACAGAGGTG GCCCATGAGG TTGGGGGTGA   481 CTCGACCGAT GTCAATGGTC AAGATGTCCC GGTCATAGTT GGGGTAAGGA GCCCGACGGG   541 CAAAGCACTC ATCCCGAGTA GGGCTGGGAG GGTGGCGGCA ACACAAGTGG TGGTGGGTCT   601 TCACAGCATA CTCCCGGTCA CAGTATTTGT CAAGGGTATC CTCCCAGGCC TTCCATGTAC   661 AGGTGTGATT GTTCCCCTGG CGGCAGCAGC GCTGGAACTC CCTCTCCAGC TGGATCAGTG   721 CCAGCAGCTC CCTTTGTAGG GGGTCAGTAG CTGGCAGGTT GCGTGGCACA GAGCGGAAGC   781 GCCTCAGGTG GCAGATGTTC TTGATATTGT CCAATGTGGG CACCCCAGGA GGGAAAGGCA   841 GCTCAAGACC CGAGGAAATA TCACGCTGAT GGCTGGGGCA GGCCCGGAGC TGGTAGTGTG   901 GCTGGGGAGC TTCCTCCTGG AAAGCAGGAG AACCGAGCCC TCCCCCTGCC GCGTGCAGCA   961 CCCAGTGGGG TCGGGTCTTG ACCGAGAACT CGGCCTCACA GAATCGGCTC ATTGCTTCTC  1021 CCCACACAAG TTTGGCACAC TCTAGCGGAT TTGTTGGTGG CTGCGGCCAG TGGCCAGCCA 1081 CCGGGAATTA TCCGAAATCC TTCCCAAAG //
Primer: SV40pro_F_V2 Sequence file: RDB13302_A5Fnd.seq
>D02166B1-4_A5Fn_SV40pro_F_V2_E11_14.ab1     1 GGAGCCTTTT TTGGAGCTAG GCTTTTGCAA AAGCTTGATT CTTCTGACAC AACAGTCTCG    61 AACTTAAGGC TAGAGCCACC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG   121 CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG   181 CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT   241 CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG   301 GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT   361 TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT   421 CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG   481 ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG   541 ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC   601 TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC   661 CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG   721 TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG   781 GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA   841 TCGCCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GGACTCTGGG GTTTCGAAAT   901 GACCCGACCC AAGCCGACGC CCAAACCTGC CATCACGAAT GGGCCGGCAA TAAAAATATC   961 CTTTTATTTT CCATAACATC TGGTGGTGTT GGCTTTTTTG GTGGTGATCG GAATAGCGAT 1021 AGAATTCGCG TATGGTGCCA CTCTCCAGTA CCACTTCTTG ACTCTGGATG GC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

♦ No Approval Forms Required for Fluorescent Protein Genes Developed by Dr. Atsushi Miyawaki
♦ RIKEN BRC will be closed from December 28, 2024 through January 5, 2025 due to New Year's holidays.
♦ A bicistronic cell cycle reporter, Fucci2a (Sep 17, 2024)
♦ Monomeric Fluorescent Protein Resource, mStayGold (Dec 18, 2023)
♦ Visualization of Organelles update (Dec 18, 2023)
♦ Development of two mouse strains conditionally expressing bright luciferases (Sep 08, 2023)
♦ Autophagy and Mitophagy Updates (Aug 16, 2023)
♦ High intensity forms of luciferase and luminescent proteins from various organisms (BRC RESOURCE NEWS) (Apr 28, 2023)
♦ Plasmid of Cas9 expression/mRNA production, evaluation of the genome edit efficiency, and Knock-in donors and tags
♦ Fucci cell cycle indicator, Calcium sensor and Fluorescent and Luminescent protein resources
♦ Revision of Distribution Fees for Bioresources in RIKEN BRC
- - - - - - - - - - - - - - - - - - - -
♦ Mail News sign-up. Receive information of the forcusd resources, new available resources and more.
♦ Please visit "Terms of Use", "Quality control" and "Ordering instruction"
    

2023.08.18

GNP_filter3_RDBDEP_html_230702.pl