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VMO1/W105F-MH (#RDB20016)

Lentivirus expression vector of human VMO1 mutant (W105F). Myc-tag and His6-tag at C-terminus.


Drawn by SnapGene® software
Sequence (full) RDB20016hts01.seq
Publication Yoshimoto, S., FEBS Open Bio. 13 (3): 490-499 (2023). PMID 36680395. [link to RRC of NBRP]
Test sheet RDB20016_B3Fgp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Lentivirus expression vector of human VMO1 mutant (W105F). Myc-tag and His6-tag at C-terminus.
Comment cDNA encoding C-terminally myc-his6-tagged human VMO1 was artificially synthesized.
Vector backbone CSII-CMV-MCS-IRES2-Bsd (RDB04385) (plasmid)
Selectable markers Ampicillin (E. coli)
Growth conditions LB+NEBstable, 30oC
Growth remarks Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r. NEB Stable or an equivalent host is recommended for amplifying this clone.
Gene/insert name human VMO1 cDNA.
Depositor|Developer Simizu, Siro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Yoshimoto, S. et al. FEBS Open Bio. 13(3): 490-499, 2023)
Additional terms and conditions:
Regarding lentivirus vector: The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用すること(Yoshimoto, S. et al. FEBS Open Bio. 13(3): 490-499, 2023)。
付加的使用条件：
レンチウイルスベクターについて：本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが三好浩之博士により開発されたことを明示する。本件リソースの使用は学術機関での学術研究に限る。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20016 VMO1/W105F-MH DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The VMO1/W105F-MH was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20016).

Reference section:

Yoshimoto, S., Suzuki, T., Otani, N., Takahashi, D., Toshima, K., Dohmae, N., Simizu, S., Destabilization of vitelline membrane outer layer protein 1 homolog (VMO1) by C-mannosylation. FEBS Open Bio. 13 (3): 490-499 (2023). PMID 36680395. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Yoshimoto, S., Destabilization of vitelline membrane outer layer protein 1 homolog (VMO1) by C-mannosylation. FEBS Open Bio. 13 (3): 490-499 (2023). PMID 36680395. [link to RRC of NBRP]