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Kazuhisa Nakayama's Resource

Kazuhisa Nakayama's web page in English /  in Japanese.

Learn more about their research, in English  /  in Japanese (by Reseachmap[link])

Catalog #Name of cloneRunning titlePublication
RDB15190pCAG2-EGFP-C1-IFT122Expression vector of human intraflagellar transport 122 (IFT122), tagged with N-terminal EGFP, CAG promoter.Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. (original). [link to RRC of NBRP]
RDB15191pCAG2-mCherry-C1-IFT122Expression vector of human intraflagellar transport 122 (IFT122), tagged with N-terminal mCherry, CAG promoter.Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. (original). [link to RRC of NBRP]
RDB15192pCAG2-EGFP-C1-IFT140Expression vector of human intraflagellar transport 140 (IFT140), tagged with N-terminal EGFP, CAG promoter.Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. (original). [link to RRC of NBRP]
RDB15193pCAG2-mCherry-C1-IFT140Expression vector of human intraflagellar transport 140 (IFT140), tagged with N-terminal mCherry, CAG promoter.Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. (original). [link to RRC of NBRP]
RDB15194pEGFP-C1-TULP3Expression vector of human tubby like protein 3 (TULP3), tagged with N-terminal EGFP, CMV promoter.Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. (original). [link to RRC of NBRP]
RDB15195pmCherry-C1-TULP3Expression vector of human tubby like protein 3 (TULP3), tagged with N-terminal mCherry, CMV promoter.Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. (original). [link to RRC of NBRP]
RDB15196pCAG-EGFP-C-KIF17Expression vector of human kinesin family member 17 (KIF17), tagged with N-terminal EGFP, CAG promoter.Funabashi, T., Ciliary entry of KIF17 is dependent on its binding to the IFT-B complex via IFT46-IFT56 as well as on its nuclear localization signal. Mol. Biol. Cell 28 (5): 624-633 (2017). PMID 28077622. (original). [link to RRC of NBRP]
RDB15197pCAG-mCherry-C-KIF17Expression vector of human kinesin family member 17 (KIF17), tagged with N-terminal mCherry, CAG promoter.Funabashi, T., Ciliary entry of KIF17 is dependent on its binding to the IFT-B complex via IFT46-IFT56 as well as on its nuclear localization signal. Mol. Biol. Cell 28 (5): 624-633 (2017). PMID 28077622. (original). [link to RRC of NBRP]
RDB15337pcDNA3/hArf1(WT)-HAExpression vector of human ARF1, wild type.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original). [link to RRC of NBRP]
RDB15338pcDNA3/mArf1(Q71L)-HAExpression vector of mouse ARF1, reduced GTPase mutant Q71L.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original). [link to RRC of NBRP]
RDB15339pcDNA3/mArf1(T31N)-HAExpression vector of mouse ARF1, low affinity for GTP mutant T31N.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original). [link to RRC of NBRP]
RDB15340pEGFP-N3/hArf1(WT)-EGFPExpression vector of human ARF1, wild type.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original). [link to RRC of NBRP]
RDB15341pcDNA3/hArf3(WT)-HAExpression vector of human ARF3, wild type.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original). [link to RRC of NBRP]
RDB15342pcDNA3/mArf3(Q71L)-HAExpression vector of mouse ARF3, reduced GTPase mutant Q71L.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original). [link to RRC of NBRP]
RDB15343pcDNA3/mArf3(T31N)-HAExpression vector of mouse ARF3, low affinity for GTP mutant T31N.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original). [link to RRC of NBRP]
RDB15344pEGFP-N3/hArf3-EGFPExpression vector of human ARF3, wild type.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original). [link to RRC of NBRP]
RDB15345pcDNA3/hArf1(WT)-mCherryExpression vector of human ARF1, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original). [link to RRC of NBRP]
RDB15346pcDNA3/hArf3-mCherryExpression vector of human ARF3, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original). [link to RRC of NBRP]
RDB15347pcDNA3/hArf5(WT)-mCherryExpression vector of human ARF5, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original). [link to RRC of NBRP]
RDB15348pEGFP-N3/hArf5(WT)-EGFPExpression vector of human ARF5, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original). [link to RRC of NBRP]
RDB15349pcDNA3/hArf6(WT)-HAExpression vector of human ARF6, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original). [link to RRC of NBRP]
RDB15350pcDNA3/hArf6(Q67L)-HAExpression vector of human ARF6, dominant activating mutant Q67L.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original). [link to RRC of NBRP]
RDB15351pcDNA3/hArf6(T27N)-HAExpression vector of human ARF6, inactivating mutant T27N.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original). [link to RRC of NBRP]
RDB15352pcDNA3/hArf6(WT)-mCherryExpression vector of human ARF6, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original). [link to RRC of NBRP]
RDB15353pEGFP-N3/hArf6(WT)-EGFPExpression vector of human ARF6, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original). [link to RRC of NBRP]
RDB15354pCAG/hArf4(WT)-HAExpression vector of human ARF4, wild type.Nakai, W., ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Mol. Biol. Cell 24 (16): 2570-2581 (2013). PMID 23783033. (original). [link to RRC of NBRP]
RDB15355pCAG/hArf4(Q71L)-HAExpression vector of human ARF4, constitutively active mutant Q71L.Nakai, W., ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Mol. Biol. Cell 24 (16): 2570-2581 (2013). PMID 23783033. (original). [link to RRC of NBRP]
RDB15356pCAG/hArf4(T31N)-HAExpression vector of human ARF4, inactive mutant T31N.Nakai, W., ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Mol. Biol. Cell 24 (16): 2570-2581 (2013). PMID 23783033. (original). [link to RRC of NBRP]
RDB15357pCAG/hArf4(WT)-mCherryExpression vector of human ARF4, wild type.Nakai, W., ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Mol. Biol. Cell 24 (16): 2570-2581 (2013). PMID 23783033. (original). [link to RRC of NBRP]
RDB15358pCAG/hArf4(WT)-EGFPExpression vector of human ARF4, wild type.Nakai, W., ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Mol. Biol. Cell 24 (16): 2570-2581 (2013). PMID 23783033. (original). [link to RRC of NBRP]
RDB15359pcDNA3/hArf5(WT)-HAExpression vector of human ARF5, wild type.Hanai, A., Class I Arfs (Arf1 and Arf3) and Arf6 are localized to the Flemming body and play important roles in cytokinesis. J. Biochem. 159 (2): 201-208 (2016). PMID 26330566. (original). [link to RRC of NBRP]
RDB15360pcDNA3/hArf5(Q71L)-HAExpression vector of mouse ARF5, constitutively active mutant Q71L.Hanai, A., Class I Arfs (Arf1 and Arf3) and Arf6 are localized to the Flemming body and play important roles in cytokinesis. J. Biochem. 159 (2): 201-208 (2016). PMID 26330566. (original). [link to RRC of NBRP]
RDB15361pcDNA3/hArf5(T31N)-HAExpression vector of mouse ARF5, inactive mutant T31N.Hanai, A., Class I Arfs (Arf1 and Arf3) and Arf6 are localized to the Flemming body and play important roles in cytokinesis. J. Biochem. 159 (2): 201-208 (2016). PMID 26330566. (original). [link to RRC of NBRP]
RDB15364pcDNA4c/His-Xpress-HA-hBIG2(WT)Expression vector of human BIG2, wild type.Ishizaki, R., Redundant roles of BIG2 and BIG1, guanine-nucleotide exchange factors for ADP-ribosylation factors in membrane traffic between the trans-Golgi network and endosomes. Mol. Biol. Cell 19 (6): 2650-2660 (2008). PMID 18417613. (original). [link to RRC of NBRP]
RDB15365pcDNA4c/His-Xpress-HA-hBIG2(E738K)Expression vector of human BIG2, dominant-negative mutant E738K.Ishizaki, R., Redundant roles of BIG2 and BIG1, guanine-nucleotide exchange factors for ADP-ribosylation factors in membrane traffic between the trans-Golgi network and endosomes. Mol. Biol. Cell 19 (6): 2650-2660 (2008). PMID 18417613. (original). [link to RRC of NBRP]
RDB15366pGEX-4T-2/hGGA1(GAT)Bacterial Expression vector of human GGA1, the GAT domain. Takatsu, H., GGA proteins associate with Golgi membranes through interaction between their GGAH domains and ADP-ribosylation factors. Biochem. J. 365 (Pt 2): 369-378 (2002). PMID 11950392. (original). [link to RRC of NBRP]
RDB15367pGEX-4T-2/hGGA3(GAT)Bacterial Expression vector of human GGA3, the GGA homology domain. Takatsu, H., GGA proteins associate with Golgi membranes through interaction between their GGAH domains and ADP-ribosylation factors. Biochem. J. 365 (Pt 2): 369-378 (2002). PMID 11950392. (original). [link to RRC of NBRP]

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2023.08.07

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