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Kazuhisa Nakayama's Resource

Kazuhisa Nakayama's web page in English /  in Japanese.

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Catalog #Name of cloneRunning titlePublication
RDB15190pCAG2-EGFP-C1-IFT122Expression vector of human intraflagellar transport 122 (IFT122), tagged with N-terminal EGFP, CAG promoter.Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. (original). [link to RRC of NBRP]
RDB15191pCAG2-mCherry-C1-IFT122Expression vector of human intraflagellar transport 122 (IFT122), tagged with N-terminal mCherry, CAG promoter.Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. (original). [link to RRC of NBRP]
RDB15192pCAG2-EGFP-C1-IFT140Expression vector of human intraflagellar transport 140 (IFT140), tagged with N-terminal EGFP, CAG promoter.Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. (original). [link to RRC of NBRP]
RDB15193pCAG2-mCherry-C1-IFT140Expression vector of human intraflagellar transport 140 (IFT140), tagged with N-terminal mCherry, CAG promoter.Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. (original). [link to RRC of NBRP]
RDB15194pEGFP-C1-TULP3Expression vector of human tubby like protein 3 (TULP3), tagged with N-terminal EGFP, CMV promoter.Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. (original). [link to RRC of NBRP]
RDB15195pmCherry-C1-TULP3Expression vector of human tubby like protein 3 (TULP3), tagged with N-terminal mCherry, CMV promoter.Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. (original). [link to RRC of NBRP]
RDB15196pCAG-EGFP-C-KIF17Expression vector of human kinesin family member 17 (KIF17), tagged with N-terminal EGFP, CAG promoter.Funabashi, T., Ciliary entry of KIF17 is dependent on its binding to the IFT-B complex via IFT46-IFT56 as well as on its nuclear localization signal. Mol. Biol. Cell 28 (5): 624-633 (2017). PMID 28077622. (original). [link to RRC of NBRP]
RDB15197pCAG-mCherry-C-KIF17Expression vector of human kinesin family member 17 (KIF17), tagged with N-terminal mCherry, CAG promoter.Funabashi, T., Ciliary entry of KIF17 is dependent on its binding to the IFT-B complex via IFT46-IFT56 as well as on its nuclear localization signal. Mol. Biol. Cell 28 (5): 624-633 (2017). PMID 28077622. (original). [link to RRC of NBRP]
RDB15337pcDNA3/hArf1(WT)-HAExpression vector of human ARF1, wild type.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original). [link to RRC of NBRP]
RDB15338pcDNA3/mArf1(Q71L)-HAExpression vector of mouse ARF1, reduced GTPase mutant Q71L.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original). [link to RRC of NBRP]
RDB15339pcDNA3/mArf1(T31N)-HAExpression vector of mouse ARF1, low affinity for GTP mutant T31N.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original). [link to RRC of NBRP]
RDB15340pEGFP-N3/hArf1(WT)-EGFPExpression vector of human ARF1, wild type.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original). [link to RRC of NBRP]
RDB15341pcDNA3/hArf3(WT)-HAExpression vector of human ARF3, wild type.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original). [link to RRC of NBRP]
RDB15342pcDNA3/mArf3(Q71L)-HAExpression vector of mouse ARF3, reduced GTPase mutant Q71L.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original). [link to RRC of NBRP]
RDB15343pcDNA3/mArf3(T31N)-HAExpression vector of mouse ARF3, low affinity for GTP mutant T31N.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original). [link to RRC of NBRP]
RDB15344pEGFP-N3/hArf3-EGFPExpression vector of human ARF3, wild type.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original). [link to RRC of NBRP]
RDB15345pcDNA3/hArf1(WT)-mCherryExpression vector of human ARF1, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original). [link to RRC of NBRP]
RDB15346pcDNA3/hArf3-mCherryExpression vector of human ARF3, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original). [link to RRC of NBRP]
RDB15347pcDNA3/hArf5(WT)-mCherryExpression vector of human ARF5, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original). [link to RRC of NBRP]
RDB15348pEGFP-N3/hArf5(WT)-EGFPExpression vector of human ARF5, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original). [link to RRC of NBRP]
RDB15349pcDNA3/hArf6(WT)-HAExpression vector of human ARF6, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original). [link to RRC of NBRP]
RDB15350pcDNA3/hArf6(Q67L)-HAExpression vector of human ARF6, dominant activating mutant Q67L.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original). [link to RRC of NBRP]
RDB15351pcDNA3/hArf6(T27N)-HAExpression vector of human ARF6, inactivating mutant T27N.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original). [link to RRC of NBRP]
RDB15352pcDNA3/hArf6(WT)-mCherryExpression vector of human ARF6, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original). [link to RRC of NBRP]
RDB15353pEGFP-N3/hArf6(WT)-EGFPExpression vector of human ARF6, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original). [link to RRC of NBRP]
RDB15354pCAG/hArf4(WT)-HAExpression vector of human ARF4, wild type.Nakai, W., ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Mol. Biol. Cell 24 (16): 2570-2581 (2013). PMID 23783033. (original). [link to RRC of NBRP]
RDB15355pCAG/hArf4(Q71L)-HAExpression vector of human ARF4, constitutively active mutant Q71L.Nakai, W., ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Mol. Biol. Cell 24 (16): 2570-2581 (2013). PMID 23783033. (original). [link to RRC of NBRP]
RDB15356pCAG/hArf4(T31N)-HAExpression vector of human ARF4, inactive mutant T31N.Nakai, W., ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Mol. Biol. Cell 24 (16): 2570-2581 (2013). PMID 23783033. (original). [link to RRC of NBRP]
RDB15357pCAG/hArf4(WT)-mCherryExpression vector of human ARF4, wild type.Nakai, W., ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Mol. Biol. Cell 24 (16): 2570-2581 (2013). PMID 23783033. (original). [link to RRC of NBRP]
RDB15358pCAG/hArf4(WT)-EGFPExpression vector of human ARF4, wild type.Nakai, W., ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Mol. Biol. Cell 24 (16): 2570-2581 (2013). PMID 23783033. (original). [link to RRC of NBRP]
RDB15359pcDNA3/hArf5(WT)-HAExpression vector of human ARF5, wild type.Hanai, A., Class I Arfs (Arf1 and Arf3) and Arf6 are localized to the Flemming body and play important roles in cytokinesis. J. Biochem. 159 (2): 201-208 (2016). PMID 26330566. (original). [link to RRC of NBRP]
RDB15360pcDNA3/hArf5(Q71L)-HAExpression vector of mouse ARF5, constitutively active mutant Q71L.Hanai, A., Class I Arfs (Arf1 and Arf3) and Arf6 are localized to the Flemming body and play important roles in cytokinesis. J. Biochem. 159 (2): 201-208 (2016). PMID 26330566. (original). [link to RRC of NBRP]
RDB15361pcDNA3/hArf5(T31N)-HAExpression vector of mouse ARF5, inactive mutant T31N.Hanai, A., Class I Arfs (Arf1 and Arf3) and Arf6 are localized to the Flemming body and play important roles in cytokinesis. J. Biochem. 159 (2): 201-208 (2016). PMID 26330566. (original). [link to RRC of NBRP]
RDB15364pcDNA4c/His-Xpress-HA-hBIG2(WT)Expression vector of human BIG2, wild type.Ishizaki, R., Redundant roles of BIG2 and BIG1, guanine-nucleotide exchange factors for ADP-ribosylation factors in membrane traffic between the trans-Golgi network and endosomes. Mol. Biol. Cell 19 (6): 2650-2660 (2008). PMID 18417613. (original). [link to RRC of NBRP]
RDB15365pcDNA4c/His-Xpress-HA-hBIG2(E738K)Expression vector of human BIG2, dominant-negative mutant E738K.Ishizaki, R., Redundant roles of BIG2 and BIG1, guanine-nucleotide exchange factors for ADP-ribosylation factors in membrane traffic between the trans-Golgi network and endosomes. Mol. Biol. Cell 19 (6): 2650-2660 (2008). PMID 18417613. (original). [link to RRC of NBRP]
RDB15366pGEX-4T-2/hGGA1(GAT)Bacterial Expression vector of human GGA1, the GAT domain. Takatsu, H., GGA proteins associate with Golgi membranes through interaction between their GGAH domains and ADP-ribosylation factors. Biochem. J. 365 (Pt 2): 369-378 (2002). PMID 11950392. (original). [link to RRC of NBRP]
RDB15367pGEX-4T-2/hGGA3(GAT)Bacterial Expression vector of human GGA3, the GGA homology domain. Takatsu, H., GGA proteins associate with Golgi membranes through interaction between their GGAH domains and ADP-ribosylation factors. Biochem. J. 365 (Pt 2): 369-378 (2002). PMID 11950392. (original). [link to RRC of NBRP]
RDB20094pcDNA3-hARFRP1(WT)-HAExpression vector of human ARFRP1 for mammalian cells, C-teminal HA-tag.Shin, H.W., Roles of ARFRP1 (ADP-ribosylation factor-related protein 1) in post-Golgi membrane trafficking. J. Cell Sci. 118 (Pt 17): 4039-4048 (2005). PMID 16129887. (original). [link to RRC of NBRP]
RDB20095pcDNA3-hARFRP1(Q79L)-HAExpression vector of human ARFRP1 mutant (Q79L) for mammalian cells, C-teminal HA-tag.Shin, H.W., Roles of ARFRP1 (ADP-ribosylation factor-related protein 1) in post-Golgi membrane trafficking. J. Cell Sci. 118 (Pt 17): 4039-4048 (2005). PMID 16129887. (original). [link to RRC of NBRP]
RDB20096pcDNA3-hARFRP1(T31N)-HAExpression vector of human ARFRP1 mutant (T31N) for mammalian cells, C-teminal HA-tag.Shin, H.W., Roles of ARFRP1 (ADP-ribosylation factor-related protein 1) in post-Golgi membrane trafficking. J. Cell Sci. 118 (Pt 17): 4039-4048 (2005). PMID 16129887. (original). [link to RRC of NBRP]
RDB20097pcDNA3-HA-hArfaptin1Expression vector of human ARFIP1 for mammalian cells, N-teminal HA-tag.Man, Z., Arfaptins are localized to the trans-Golgi by interaction with Arl1, but not Arfs. J. Biol. Chem. 286 (13): 11569-11578 (2011). PMID 21239483. (original). [link to RRC of NBRP]
RDB20098pcDNA3-HA-hArfaptin2Expression vector of human ARFIP2 for mammalian cells, N-teminal HA-tag.Man, Z., Arfaptins are localized to the trans-Golgi by interaction with Arl1, but not Arfs. J. Biol. Chem. 286 (13): 11569-11578 (2011). PMID 21239483. (original). [link to RRC of NBRP]
RDB20099EGFP-hArfaptin1Expression vector of human ARFIP1 for mammalian cells, N-teminal EGFP-tag.Man, Z., Arfaptins are localized to the trans-Golgi by interaction with Arl1, but not Arfs. J. Biol. Chem. 286 (13): 11569-11578 (2011). PMID 21239483. (original). [link to RRC of NBRP]
RDB20100EGFP-hArfaptin2Expression vector of human ARFIP2 for mammalian cells, N-teminal EGFP-tag.Man, Z., Arfaptins are localized to the trans-Golgi by interaction with Arl1, but not Arfs. J. Biol. Chem. 286 (13): 11569-11578 (2011). PMID 21239483. (original). [link to RRC of NBRP]
RDB20101hARL1(WT)-EGFPExpression vector of human ARL1 for mammalian cells, C-teminal EGFP-tag.Man, Z., Arfaptins are localized to the trans-Golgi by interaction with Arl1, but not Arfs. J. Biol. Chem. 286 (13): 11569-11578 (2011). PMID 21239483. (original). [link to RRC of NBRP]
RDB20102hARL1(Q71L)-EGFPExpression vector of human ARL1 mutant (Q71L) for mammalian cells, C-teminal EGFP-tag.Man, Z., Arfaptins are localized to the trans-Golgi by interaction with Arl1, but not Arfs. J. Biol. Chem. 286 (13): 11569-11578 (2011). PMID 21239483. (original). [link to RRC of NBRP]
RDB20103hARL1(T31N)-EGFPExpression vector of human ARL1 mutant (T31N) for mammalian cells, C-teminal EGFP-tag.Man, Z., Arfaptins are localized to the trans-Golgi by interaction with Arl1, but not Arfs. J. Biol. Chem. 286 (13): 11569-11578 (2011). PMID 21239483. (original). [link to RRC of NBRP]
RDB20104pcDNA3-hARFGAP1-HAExpression vector of human ARFGAP1 for mammalian cells, C-teminal HA-tag.Saitoh, A., Three homologous ArfGAPs participate in coat protein I-mediated transport. J. Biol. Chem. 284 (20): 13948-13957 (2009). PMID 19299515. (original). [link to RRC of NBRP]
RDB20105pcDNA3-hARFGAP2-HAExpression vector of human ARFGAP2 for mammalian cells, C-teminal HA-tag.Saitoh, A., Three homologous ArfGAPs participate in coat protein I-mediated transport. J. Biol. Chem. 284 (20): 13948-13957 (2009). PMID 19299515. (original). [link to RRC of NBRP]
RDB20106pcDNA3-hARFGAP3-HAExpression vector of human ARFGAP3 for mammalian cells, C-teminal HA-tag.Saitoh, A., Three homologous ArfGAPs participate in coat protein I-mediated transport. J. Biol. Chem. 284 (20): 13948-13957 (2009). PMID 19299515. (original). [link to RRC of NBRP]
RDB20107EGFP-VSVG-KDELR2Expression vector for mammalian cells. Preproglucagon signal sequence, EGFP, luminal region of VSVG (tsO45), TM and cytoplasmic region of human KDELR2.Saitoh, A., Three homologous ArfGAPs participate in coat protein I-mediated transport. J. Biol. Chem. 284 (20): 13948-13957 (2009). PMID 19299515. (original). [link to RRC of NBRP]
RDB20108EGFP-hEFA6AExpression vector of human EFA6A for mammalian cells, N-teminal EGFP-tag.Ueda, T., EFA6 activates Arf6 and participates in its targeting to the Flemming body during cytokinesis. FEBS Lett. 587 (11): 1617-1623 (2013). PMID 23603394. (original). [link to RRC of NBRP]
RDB20109EGFP-hEFA6BExpression vector of human EFA6B for mammalian cells, N-teminal EGFP-tag.Ueda, T., EFA6 activates Arf6 and participates in its targeting to the Flemming body during cytokinesis. FEBS Lett. 587 (11): 1617-1623 (2013). PMID 23603394. (original). [link to RRC of NBRP]
RDB20110EGFP-hEFA6CExpression vector of human EFA6C for mammalian cells, N-teminal EGFP-tag.Ueda, T., EFA6 activates Arf6 and participates in its targeting to the Flemming body during cytokinesis. FEBS Lett. 587 (11): 1617-1623 (2013). PMID 23603394. (original). [link to RRC of NBRP]
RDB20111pcDNA3-HA-GBF1Expression vector of human GBF1 for mammalian cells, N-teminal HA-tag.Kawamoto, K., GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat. Traffic 3 (7): 483-495 (2002). PMID 12047556. (original). [link to RRC of NBRP]
RDB20112pcDNA3-HA-GBF1(E794K)Expression vector of human GBF1 mutant (E794K) for mammalian cells, N-teminal HA-tag.Kawamoto, K., GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat. Traffic 3 (7): 483-495 (2002). PMID 12047556. (original). [link to RRC of NBRP]
RDB20113pcDNA4-Xpress-GBF1Expression vector of human GBF1 for mammalian cells, N-teminal Xpress-tag.Kawamoto, K., GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat. Traffic 3 (7): 483-495 (2002). PMID 12047556. (original). [link to RRC of NBRP]
RDB20114pcDNA4-Xpress-GBF1(E794K)Expression vector of human GBF1 mutant (E794K) for mammalian cells, N-teminal Xpress-tag.Kawamoto, K., GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat. Traffic 3 (7): 483-495 (2002). PMID 12047556. (original). [link to RRC of NBRP]
RDB20234pCAG-EGFP-C-IFT70BExpression vector of human IFT70B, tagged with EGFP at N-terminus, CAG promoter.Katoh, Y., Overall Architecture of the Intraflagellar Transport (IFT)-B Complex Containing Cluap1/IFT38 as an Essential Component of the IFT-B Peripheral Subcomplex. J. Biol. Chem. 291 (21): 10962-10975 (2016). PMID 26980730. (original). [link to RRC of NBRP]
RDB20235pEGFP-C1-IFT54Expression vector of human IFT54, tagged with EGFP at N-terminus, CMV promoter.Katoh, Y., Overall Architecture of the Intraflagellar Transport (IFT)-B Complex Containing Cluap1/IFT38 as an Essential Component of the IFT-B Peripheral Subcomplex. J. Biol. Chem. 291 (21): 10962-10975 (2016). PMID 26980730. (original). [link to RRC of NBRP]
RDB20236pEGFP-C1-FOP(FGFR1OP)Expression vector of human FOP/FGFR1OP, tagged with EGFP at N-terminus, CMV promoter.Nishijima, Y., RABL2 interacts with the intraflagellar transport-B complex and CEP19 and participates in ciliary assembly. Mol. Biol. Cell 28 (12): 1652-1666 (2017). PMID 28428259. (original). [link to RRC of NBRP]
RDB20237pCAG2-EGFP-C-KAP3Expression vector of human KAP3, tagged with EGFP at N-terminus, CAG promoter.Funabashi, T., Interaction of heterotrimeric kinesin-II with IFT-B-connecting tetramer is crucial for ciliogenesis. J. Cell Biol. 217 (8): 2867-2876 (2018). PMID 29903877. (original). [link to RRC of NBRP]
RDB20238pCAG2-EGFP-C-KIF3AExpression vector of human KIF3A, tagged with EGFP at N-terminus, CAG promoter.Funabashi, T., Interaction of heterotrimeric kinesin-II with IFT-B-connecting tetramer is crucial for ciliogenesis. J. Cell Biol. 217 (8): 2867-2876 (2018). PMID 29903877. (original). [link to RRC of NBRP]
RDB20239pCAG2-EGFP-C-DYNC2LI1Expression vector of human DYNC2LI1, tagged with EGFP at N-terminus, CAG promoter.Hamada, Y., Interaction of WDR60 intermediate chain with TCTEX1D2 light chain of the dynein-2 complex is crucial for ciliary protein trafficking. Mol. Biol. Cell 29 (13): 1628-1639 (2018). PMID 29742051. (original). [link to RRC of NBRP]
RDB20240pCAG2-EGFP-C-WDR34Expression vector of human WDR34, tagged with EGFP at N-terminus, CAG promoter.Hamada, Y., Interaction of WDR60 intermediate chain with TCTEX1D2 light chain of the dynein-2 complex is crucial for ciliary protein trafficking. Mol. Biol. Cell 29 (13): 1628-1639 (2018). PMID 29742051. (original). [link to RRC of NBRP]
RDB20241pCAG2-EGFP-C-WDR60Expression vector of human WDR60, tagged with EGFP at N-terminus, CAG promoter.Hamada, Y., Interaction of WDR60 intermediate chain with TCTEX1D2 light chain of the dynein-2 complex is crucial for ciliary protein trafficking. Mol. Biol. Cell 29 (13): 1628-1639 (2018). PMID 29742051. (original). [link to RRC of NBRP]

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2024.05.08

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