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Kazuhisa Nakayama's Resource

Kazuhisa Nakayama's web page in English    in Japanese.

Learn more about their research in Reseachmap[link]   in English     in Japanese.

Catalog #Name of cloneRunning titlePublication
RDB15190pCAG2-EGFP-C1-IFT122Expression vector of human intraflagellar transport 122 (IFT122), tagged with N-terminal EGFP, CAG promoter.Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. (original).
RDB15191pCAG2-mCherry-C1-IFT122Expression vector of human intraflagellar transport 122 (IFT122), tagged with N-terminal mCherry, CAG promoter.Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. (original).
RDB15192pCAG2-EGFP-C1-IFT140Expression vector of human intraflagellar transport 140 (IFT140), tagged with N-terminal EGFP, CAG promoter.Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. (original).
RDB15193pCAG2-mCherry-C1-IFT140Expression vector of human intraflagellar transport 140 (IFT140), tagged with N-terminal mCherry, CAG promoter.Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. (original).
RDB15194pEGFP-C1-TULP3Expression vector of human tubby like protein 3 (TULP3), tagged with N-terminal EGFP, CMV promoter.Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. (original).
RDB15195pmCherry-C1-TULP3Expression vector of human tubby like protein 3 (TULP3), tagged with N-terminal mCherry, CMV promoter.Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. (original).
RDB15196pCAG-EGFP-C-KIF17Expression vector of human kinesin family member 17 (KIF17), tagged with N-terminal EGFP, CAG promoter.Funabashi, T., Ciliary entry of KIF17 is dependent on its binding to the IFT-B complex via IFT46-IFT56 as well as on its nuclear localization signal. Mol. Biol. Cell 28 (5): 624-633 (2017). PMID 28077622. (original).
RDB15197pCAG-mCherry-C-KIF17Expression vector of human kinesin family member 17 (KIF17), tagged with N-terminal mCherry, CAG promoter.Funabashi, T., Ciliary entry of KIF17 is dependent on its binding to the IFT-B complex via IFT46-IFT56 as well as on its nuclear localization signal. Mol. Biol. Cell 28 (5): 624-633 (2017). PMID 28077622. (original).
RDB15337pcDNA3/hArf1(WT)-HAExpression vector of human ARF1, wild type.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original).
RDB15338pcDNA3/mArf1(Q71L)-HAExpression vector of mouse ARF1, reduced GTPase mutant Q71L.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original).
RDB15339pcDNA3/mArf1(T31N)-HAExpression vector of mouse ARF1, low affinity for GTP mutant T31N.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original).
RDB15340pEGFP-N3/hArf1(WT)-EGFPExpression vector of human ARF1, wild type.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original).
RDB15341pcDNA3/hArf3(WT)-HAExpression vector of human ARF3, wild type.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original).
RDB15342pcDNA3/mArf3(Q71L)-HAExpression vector of mouse ARF3, reduced GTPase mutant Q71L.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original).
RDB15343pcDNA3/mArf3(T31N)-HAExpression vector of mouse ARF3, low affinity for GTP mutant T31N.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original).
RDB15344pEGFP-N3/hArf3-EGFPExpression vector of human ARF3, wild type.Kondo, Y., ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway. Cell Struct. Funct. 37 (2): 141-154 (2012). PMID 22971977. (original).
RDB15345pcDNA3/hArf1(WT)-mCherryExpression vector of human ARF1, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original).
RDB15346pcDNA3/hArf3-mCherryExpression vector of human ARF3, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original).
RDB15347pcDNA3/hArf5(WT)-mCherryExpression vector of human ARF5, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original).
RDB15348pEGFP-N3/hArf5(WT)-EGFPExpression vector of human ARF5, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original).
RDB15349pcDNA3/hArf6(WT)-HAExpression vector of human ARF6, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original).
RDB15350pcDNA3/hArf6(Q67L)-HAExpression vector of human ARF6, dominant activating mutant Q67L.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original).
RDB15351pcDNA3/hArf6(T27N)-HAExpression vector of human ARF6, inactivating mutant T27N.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original).
RDB15352pcDNA3/hArf6(WT)-mCherryExpression vector of human ARF6, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original).
RDB15353pEGFP-N3/hArf6(WT)-EGFPExpression vector of human ARF6, wild type.Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. (original).
RDB15354pCAG/hArf4(WT)-HAExpression vector of human ARF4, wild type.Nakai, W., ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Mol. Biol. Cell 24 (16): 2570-2581 (2013). PMID 23783033. (original).
RDB15355pCAG/hArf4(Q71L)-HAExpression vector of human ARF4, constitutively active mutant Q71L.Nakai, W., ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Mol. Biol. Cell 24 (16): 2570-2581 (2013). PMID 23783033. (original).
RDB15356pCAG/hArf4(T31N)-HAExpression vector of human ARF4, inactive mutant T31N.Nakai, W., ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Mol. Biol. Cell 24 (16): 2570-2581 (2013). PMID 23783033. (original).
RDB15357pCAG/hArf4(WT)-mCherryExpression vector of human ARF4, wild type.Nakai, W., ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Mol. Biol. Cell 24 (16): 2570-2581 (2013). PMID 23783033. (original).
RDB15358pCAG/hArf4(WT)-EGFPExpression vector of human ARF4, wild type.Nakai, W., ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Mol. Biol. Cell 24 (16): 2570-2581 (2013). PMID 23783033. (original).
RDB15359pcDNA3/hArf5(WT)-HAExpression vector of human ARF5, wild type.Hanai, A., Class I Arfs (Arf1 and Arf3) and Arf6 are localized to the Flemming body and play important roles in cytokinesis. J. Biochem. 159 (2): 201-208 (2016). PMID 26330566. (original).
RDB15360pcDNA3/hArf5(Q71L)-HAExpression vector of mouse ARF5, constitutively active mutant Q71L.Hanai, A., Class I Arfs (Arf1 and Arf3) and Arf6 are localized to the Flemming body and play important roles in cytokinesis. J. Biochem. 159 (2): 201-208 (2016). PMID 26330566. (original).
RDB15361pcDNA3/hArf5(T31N)-HAExpression vector of mouse ARF5, inactive mutant T31N.Hanai, A., Class I Arfs (Arf1 and Arf3) and Arf6 are localized to the Flemming body and play important roles in cytokinesis. J. Biochem. 159 (2): 201-208 (2016). PMID 26330566. (original).
RDB15362pcDNA4c/His-Xpress-HA-hBIG1(WT)Expression vector of human BIG1, wild type.Ishizaki, R., Redundant roles of BIG2 and BIG1, guanine-nucleotide exchange factors for ADP-ribosylation factors in membrane traffic between the trans-Golgi network and endosomes. Mol. Biol. Cell 19 (6): 2650-2660 (2008). PMID 18417613. (original).
RDB15363pcDNA4c/His-Xpress-HA-hBIG1(E793K)Expression vector of human BIG1, dominant-negative mutant E793K.Ishizaki, R., Redundant roles of BIG2 and BIG1, guanine-nucleotide exchange factors for ADP-ribosylation factors in membrane traffic between the trans-Golgi network and endosomes. Mol. Biol. Cell 19 (6): 2650-2660 (2008). PMID 18417613. (original).
RDB15364pcDNA4c/His-Xpress-HA-hBIG2(WT)Expression vector of human BIG2, wild type.Ishizaki, R., Redundant roles of BIG2 and BIG1, guanine-nucleotide exchange factors for ADP-ribosylation factors in membrane traffic between the trans-Golgi network and endosomes. Mol. Biol. Cell 19 (6): 2650-2660 (2008). PMID 18417613. (original).
RDB15365pcDNA4c/His-Xpress-HA-hBIG2(E738K)Expression vector of human BIG2, dominant-negative mutant E738K.Ishizaki, R., Redundant roles of BIG2 and BIG1, guanine-nucleotide exchange factors for ADP-ribosylation factors in membrane traffic between the trans-Golgi network and endosomes. Mol. Biol. Cell 19 (6): 2650-2660 (2008). PMID 18417613. (original).
RDB15366pGEX-4T-2/hGGA1(GAT)Bacterial Expression vector of human GGA1, the GAT domain. Takatsu, H., GGA proteins associate with Golgi membranes through interaction between their GGAH domains and ADP-ribosylation factors. Biochem. J. 365 (Pt 2): 369-378 (2002). PMID 11950392. (original).
RDB15367pGEX-4T-2/hGGA3(GAT)Bacterial Expression vector of human GGA3, the GGA homology domain. Takatsu, H., GGA proteins associate with Golgi membranes through interaction between their GGAH domains and ADP-ribosylation factors. Biochem. J. 365 (Pt 2): 369-378 (2002). PMID 11950392. (original).

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2020.10.02

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