RDB15350 - pcDNA3/hArf6(Q67L)-HA

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pcDNA3/hArf6(Q67L)-HA

Expression vector of human ARF6, dominant activating mutant Q67L.

Catalog number	RDB15350
Resource name	pcDNA3/hArf6(Q67L)-HA
Alternative name	W54
Clone info.	Expression vector of human ADP-ribosylation factor6 (ARF6), dominant activating mutant Q67L, tagged with HA epitope at C-ter.
Comment	Expression was confirmed by the depositor with immunostaining.
Vector backbone	pcDNA3 (plasmid)
Size of vector backbone	5.4 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cels).
Gene/insert name	human ARF6 cDNA
Depositor\|Developer	Nakayama, Kazuhisa \|

Other clones in our bank

human ARF6

External Database
human ARF6

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makyio, H. et al. EMBO. J., 31 (11): 2590-2603, 2012). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makyio, H. et al. EMBO. J., 31 (11): 2590-2603, 2012). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makyio, H. et al. EMBO. J., 31 (11): 2590-2603, 2012)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15350	pcDNA3/hArf6(Q67L)-HA	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original	Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15350_A7I5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro, insert (hArf6 (Q67L)), HA Sequence file: RDB15350_A7I5a.seq
>D03588B5_A7I5_1_CMV_Forward_C03_09_ABI24.ab1 1 NNNNNNNNNN NNNNNTNNNN GCNNTGGGCG GTANGCGTGT ACGGTGGGAN GTCTATATAA 61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA 121 CTCACTATAG GGAGACCCAA GCTTGGTACC GAGCTCGGAT CTGCCACCAT GGGGAAGGTG 181 CTATCCAAAA TCTTCGGGAA CAAGGAAATG CGGATCCTCA TGTTGGGCCT GGACGCGGCC 241 GGCAAGACAA CAATCCTGTA CAAGTTGAAG CTGGGCCAGT CGGTGACCAC CATTCCCACT 301 GTGGGTTTCA ACGTGGAGAC GGTGACTTAC AAAAATGTCA AGTTCAACGT ATGGGATGTG 361 GGCGGCCTGG ACAAGATCCG GCCGCTCTGG CGGCATTACT ACACTGGGAC CCAAGGTCTC 421 ATCTTCGTAG TGGACTGCGC CGACCGCGAC CGCATCGATG AGGCTCGCCA GGAGCTGCAC 481 CGCATTATCA ATGACCGGGA GATGAGGGAC GCCATAATCC TCATCTTCGC CAACAAGCAG 541 GACCTGCCCG ATGCCATGAA ACCCCACGAG ATCCAGGAGA AACTGGGCCT GACCCGGATT 601 CGGGACAGGA ACTGGTATGT GCAGCCCTCC TGTGCCACCT CAGGGGACGG ACTCTATGAG 661 GGGCTCACAT GGTTAACCTC TAACTACAAA TCTCTCGAGT ATCCATACGA TGTACCAGAC 721 TACGCAGAGN TCCACTAGTT CTAGAGGGCC CTATTCTATA GTGTCACCTA AATGCTAGAG 781 CTCGCTGATC AGCCTCNNCT GTGCCTTCTA GTTGCCAGCC ATCTGTTGNT TGCCCCTCCC 841 CCCNNGCCTT CCTTGACCCT GNAANGTGCC ACTCCNACNG NCCTTTCCNN ANNAAANGAN 901 GNAANTGCAT CGCNNNNNCT NANNNNGNNN CNNTCTATNC TGGGGGGGGN NGGGNNNGGN 961 NNNNNNNNN //
Primer: SV40pro_F Region: NeoR Sequence file: RDB15350_A7I5b.seq
>D03588B5_1_A7I5_SV40pro_F_H03_24_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNAN NNGTGNNGNN GNTTTTTTGG AGGCCTAGGC TTTTGCAAAA 61 AGCTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT 121 CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT 181 ATCTTATCAT GTCTGGATCC GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT 241 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG 301 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG 361 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG 421 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 481 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT 541 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 601 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTANGTA TCTCAGTTCG 661 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 721 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 781 CTGGCAGCAG CCACTGGTAA CAGGNNTAGC AGANCGAGGT ATGTANGCGG TGCTACAGAN 841 TTCTTGAAGT GGTGGCCNNA CTACNGCTAC ACTAGAAGAA CAGTATTNGG TATCTGCGCN 901 CTGCTGAAGC NNGTNANCTT CGGAAAAAGN NTTGGTANCT NNNGNTCCNG CAAANNAACC 961 NNNGCNGGNA NCNGNNNGNT TTTTTNNNTN NNNNNCNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702.

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl