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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3/hArf6(Q67L)-HA (#RDB15350)

Expression vector of human ARF6, dominant activating mutant Q67L.

Alternative name W54
Clone info. Expression vector of human ADP-ribosylation factor6 (ARF6), dominant activating mutant Q67L, tagged with HA epitope at C-ter.
Comment Expression was confirmed by the depositor with immunostaining.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cels).
Gene/insert name human ARF6 cDNA
Depositor|Developer Nakayama, Kazuhisa |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makyio, H. et al. EMBO. J., 31 (11): 2590-2603, 2012). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makyio, H. et al. EMBO. J., 31 (11): 2590-2603, 2012)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15350 pcDNA3/hArf6(Q67L)-HA DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3/hArf6(Q67L)-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15350).

Reference section:

Makyio, H., Ohgi, M., Takei, T., Takahashi, S., Takatsu, H., Katoh, Y., Hanai, A., Ueda, T., Kanaho, Y., Xie, Y., Shin, H.W., Kamikubo, H., Kataoka, M., Kawasaki, M., Kato, R., Wakatsuki, S., Nakayama, K., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15350_A7I5p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, insert (hArf6 (Q67L)), HA
Sequence file: RDB15350_A7I5a.seq check
>D03588B5_A7I5_1_CMV_Forward_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNTNNNN GCNNTGGGCG GTANGCGTGT ACGGTGGGAN GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTTGGTACC GAGCTCGGAT CTGCCACCAT GGGGAAGGTG
  181 CTATCCAAAA TCTTCGGGAA CAAGGAAATG CGGATCCTCA TGTTGGGCCT GGACGCGGCC
  241 GGCAAGACAA CAATCCTGTA CAAGTTGAAG CTGGGCCAGT CGGTGACCAC CATTCCCACT
  301 GTGGGTTTCA ACGTGGAGAC GGTGACTTAC AAAAATGTCA AGTTCAACGT ATGGGATGTG
  361 GGCGGCCTGG ACAAGATCCG GCCGCTCTGG CGGCATTACT ACACTGGGAC CCAAGGTCTC
  421 ATCTTCGTAG TGGACTGCGC CGACCGCGAC CGCATCGATG AGGCTCGCCA GGAGCTGCAC
  481 CGCATTATCA ATGACCGGGA GATGAGGGAC GCCATAATCC TCATCTTCGC CAACAAGCAG
  541 GACCTGCCCG ATGCCATGAA ACCCCACGAG ATCCAGGAGA AACTGGGCCT GACCCGGATT
  601 CGGGACAGGA ACTGGTATGT GCAGCCCTCC TGTGCCACCT CAGGGGACGG ACTCTATGAG
  661 GGGCTCACAT GGTTAACCTC TAACTACAAA TCTCTCGAGT ATCCATACGA TGTACCAGAC
  721 TACGCAGAGN TCCACTAGTT CTAGAGGGCC CTATTCTATA GTGTCACCTA AATGCTAGAG
  781 CTCGCTGATC AGCCTCNNCT GTGCCTTCTA GTTGCCAGCC ATCTGTTGNT TGCCCCTCCC
  841 CCCNNGCCTT CCTTGACCCT GNAANGTGCC ACTCCNACNG NCCTTTCCNN ANNAAANGAN
  901 GNAANTGCAT CGCNNNNNCT NANNNNGNNN CNNTCTATNC TGGGGGGGGN NGGGNNNGGN
961 NNNNNNNNN
//
Primer: SV40pro_F (Pr0110)
Region: NeoR
Sequence file: RDB15350_A7I5b.seq check
>D03588B5_1_A7I5_SV40pro_F_H03_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNAN NNGTGNNGNN GNTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT
  121 CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT
  181 ATCTTATCAT GTCTGGATCC GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT
  241 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG
  301 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
  361 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG
  421 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
  481 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
  541 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
  601 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTANGTA TCTCAGTTCG
  661 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC
  721 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
  781 CTGGCAGCAG CCACTGGTAA CAGGNNTAGC AGANCGAGGT ATGTANGCGG TGCTACAGAN
  841 TTCTTGAAGT GGTGGCCNNA CTACNGCTAC ACTAGAAGAA CAGTATTNGG TATCTGCGCN
  901 CTGCTGAAGC NNGTNANCTT CGGAAAAAGN NTTGGTANCT NNNGNTCCNG CAAANNAACC
961 NNNGCNGGNA NCNGNNNGNT TTTTTNNNTN NNNNNCNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. [link to RRC of NBRP]