Please review the QC test results indicated by icon below as well as clone information before placing your order.

pmCherry-C1-TULP3

Expression vector of human tubby like protein 3 (TULP3), tagged with N-terminal mCherry, CMV promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes requires a separate license from the DEPOSITOR, prior to distribution.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Remarks	This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。本件リソースを学術研究以外に利用する場合は、事前に寄託者との間で、別途使用許諾にかかる契約の締結が必要である。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15195_B3Gkp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_F (Pr0453) Region: mCherry 3',insert 5' Sequence file: RDB15195_B3Gke.seq
>D04646A6_B3Gk_1_mCherry-C_F_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCNG CCNNNNNCCT ACNACGTNNA CATCAAGTTG GACATCACCT    61 CCCACAACGN NNNNNNNNNN NTCNNGGAAC AGTACGAACG CGCCGAGGGC CGNNNCTCCA   121 CCGGCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC TATGGAGGCT TCGCGCTGCC   181 GGCTCAGTCC CAGCGGCGAC AGTGTCTTCC ATGAAGAAAT GATGAAGATG CGACAGGCTA   241 AGCTGGATTA TCAGAGGCTA CTACTTGAGA AGAGGCAAAG GAAAAAGCGC CTTGAGCCAT   301 TTATGGTGCA GCCCAATCCA GAAGCCAGGC TACGTCGGGC AAAGCCAAGG GCCAGTGATG   361 AGCAGACTCC CTTGGTGAAC TGTCATACTC CCCACAGCAA TGTCATCTTA CATGGTATTG   421 ATGGTCCAGC TGCTGTCCTG AAACCAGACG AAGTTCATGC TCCATCAGTA AGCTCCTCTG   481 TTGTGGAAGA AGATGCTGAA AACACCGTGG ATACTGCTTC CAAGCCAGGA CTTCAGGAGC   541 GTCTCCAAAA GCATGATATC TCTGAAAGTG TGAACTTCGA TGAGGAGACT GATGGAATAT   601 CCCAGTCAGC ATGTTTAGAA AGACCCAATT CTGCATCAAG CCAGAATTCA ACCGATACAG   661 GCACTTCCGG TTCTGCTACT GCCGCCCAAC CAGCTGATAA CCTCCTGGGA GACATAGACT   721 ACCTGGAGGA NTTTGTGTAT AGTCCTGCCC CTCAAGGTGT CACAGTAAGA TGTCGGATAA   781 TCCGGGATAA AAGGGGAATG GATCGNGGTC TCTTCCCNAC CTACTATATG TACTTGGAAA   841 AAGAAGAAAA TCAGAAGATA TTTCNTCTTG CAGCTAGAAA GNNGAAAAAG ANCAAAACAG   901 CCNANTANCT TATCTNCNNN NNTCCNNNNN ANTTATNNCN NGNANNNNNA AAGNTNNGNN 961 NNNNNNNN //
Primer: SV40LPA-F2 (Pr1235) Region: insert 3' Sequence file: RDB15195_B3Gkf.seq
>D04646A6_B3Gk_1_SV40LPA-F2_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTN NCANCNACNA TTGCATTCAT TTTATGTTTC    61 NGGTTCAGGG GGNGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA   121 TGGCTGATTA TGATCAGTTA TCTAGATCCG GTGGATCCCG GGCCCGCGGT ACCGTCGACT   181 CATTCACACG CCAGCTTACT GTCAAAGCTA GAAAGACCGA TGCCAAAGGC CTGTACTGCA   241 CAAAGTGGGT AGTTGTAATC CAGTGTGAAC ACGTCATCTG CCACACGTCC AAACTGCATG   301 ACTATATAAT CAGGGTCATT TTTGTGGACT ATCTGGAAGT TCTTCACAGA CGCCTGAGTG   361 ACCCGGCCAC GGAAGTTGAG GACATAGGAC TGAGTGTCAC TGTTCCAGAC GGGGGCCTTG   421 TTGTGCAGCT CAACCAGATT TTCCATAGTT CTGTTCTGCC ACCTTGAGAG CAAACTGTCA   481 TGGTTGTTTT GTGGCTGATA GGGGATCTGC TTATGATTCA GTGTCATTCC AGGAATGATC   541 ACAGACATTT TCCTAGGACC TTTAAATCCA AGTACGTTTG TTTCATAGGA GATGGCAGCC   601 AGCTCCTGCC GGGTGTGGGC CGCTCCTACC AAACCCCGGC CCTTCATGGG GCAGATGCCA   661 CGGTCATAAA CTGTAAACTT GGTCCCCATG ANGTTGGATC TAAGCTTGCC GACATAACTT   721 TCTCCTTCAC GANATAAATC AACTGGATCA ATGGAGATAA NGTAGTTGGC TGTTTTGCTC   781 TTTTTCCGCT TTCTANCTGC AAGAANAANT ATCTTCTGAN TTTCTTCTTT TTTNCAAGTN   841 CATATAGTNN NNGGGGGAAN ANANCCCNNN NCCNNTNNCC NNTTTNANCC CNGANNNNNN 901 NNNN //
Primer: CMV_Forward (Pr0016) Region: mCherry 5' Sequence file: RDB15195_B3Gkg.seq
>D04646A6_B3Gk_1_CMV_Forward_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGGCGGTANG CGTGTNCGGT GGGAGGTCTA    61 TATAAGCAGA GCTGGNNTAG TGAACCGTCA GATCCGCTAG CGCCACCATG GTGAGCAAGG   121 GCGAGGAGGA TAACATGGCC ATCATCAAGG AGTTCATGCG CTTCAAGGTG CACATGGAGG   181 GCTCCGTGAA CGGCCACGAG TTCGAGATCG AGGGCGAGGG CGAGGGCCGC CCCTACGAGG   241 GCACCCAGAC CGCCAAGCTG AAGGTGACCA AGGGTGGCCC CCTGCCCTTC GCCTGGGACA   301 TCCTGTCCCC TCAGTTCATG TACGGCTCCA AGGCCTACGT GAAGCACCCC GCCGACATCC   361 CCGACTACTT GAAGCTGTCC TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG ATGAACTTCG   421 AGGACGGCGG CGTGGTGACC GTGACCCAGG ACTCCTCCCT GCAGGACGGC GAGTTCATCT   481 ACAAGGTGAA GCTGCGCGGC ACCAACTTCC CCTCCGACGG CCCCGTAATG CAGAAGAAGA   541 CCATGGGCTG GGAGGCCTCC TCCGAGCGGA TGTACCCCGA GGACGGCGCC CTGAAGGGCG   601 AGATCAAGCA GAGGCTGAAG CTGAAGGACG GCGGCCACTA CGACGCTGAG GTCAAGACCA   661 CCTACAAGGC CAAGAAGCCC GTGCAGCTGC CCGGCGCCTA CAACGTCAAC ATCAAGTTGG   721 ACATCACCTC CCACAACGAG GNNTACACCA TCGTGNAACA GTACGAACGC GCCGAGGGCC   781 GCCACTCCAC CGGCGGCATG GACGANCTGT ACAAGTNCGG ANTCANATCT ATGGAGGCTT   841 CGCGCTGCCN GCTCAGNCCC AGCGGCGANN GTGTCTTCCN TGAANAAANG ATGNNNATGC 901 NACNNGNTNN NCTNGNATTA TCANNNNNNA CTANNNNNNN NNANNNNANN NNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: NeoR_KanR Sequence file: RDB15195_B3Gkh.seq
>D04646A6_B3Gk_1_SV40pro_ori_F_H02_23_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNCCTCNG CCTCNGNNCT NNTCNNGAAG TAGTGAGGAG    61 GCTTTTTTGG ANNCNNNNNN TTTTGCAAAG ATCGATCAAG AGACAGGATG AGGATNNTTT   121 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA   181 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG   241 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA   301 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT   361 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG   421 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA   481 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT   541 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC   601 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC   661 GACGGCGANG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGNNN   721 NNGNCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAN CGCTATCANG   781 ACATANCGTT GGCTANCCGN GANATTGCTG AANANCTTGG CGGCGAATGG GCTGNNCGCT   841 TCCTCNNGCT TTANGGNATC GCCGCTCCCN ATTNNCANCG NATCNCCNTC NATCNNNNNN   901 NNGNNNANNN CNTNNNNANC NGNNNTNNNG GNNNTNNNNA NNNNNCNNNN NNANNNNNNN 961 NCCNNNNNNN NCNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.07

GNP_filter3_RDBDEP_html_230702.pl