Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCAG2-EGFP-C1-IFT122

Expression vector of human intraflagellar transport 122 (IFT122), tagged with N-terminal EGFP, CAG promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-199, 1991). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes requires a separate license from the DEPOSITOR, prior to distribution.
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-199, 1991)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。本件リソースを学術研究以外に利用する場合は、事前に寄託者との間で、別途使用許諾にかかる契約の締結が必要である。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 [Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15190_A7Fsp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C Region: EGFP, insert 5' Sequence file: RDB15190_A7Fsa.seq
>D04646A1_A7Fs_1_EGFP-C_A07_01_ABI24.ab1     1 ACGGGTATCT CGGCATGGAC GAGCTGTACA GTCCGGACTC AGATCTCGAG CTCAAGCTTC    61 GAATTCTATG AGGGCCGTGT TGACGTGGAG AGATAAAGCC GAGCACTGTA TAAATGACAT   121 CGCATTTAAG CCTGATGGAA CTCAACTGAT TTTGGCTGCC GGAAGCAGAT TACTGGTTTA   181 TGACACCTCT GATGGCACCT TACTTCAGCC CCTCAAGGGA CACAAAGACA CTGTGTACTG   241 TGTGGCATAT GCGAAGGATG GCAAGCGCTT TGCTTCTGGA TCAGCTGACA AAAGCGTTAT   301 TATCTGGACA TCAAAACTGG AAGGCATTCT GAAGTACACG CACAATGATG CTATACAATG   361 TGTCTCCTAC AATCCTATTA CTCATCAACT GGCATCTTGT TCCTCCAGTG ACTTTGGGTT   421 GTGGTCTCCT GAACAGAAGT CTGTCTCCAA ACACAAATCA AGCAGCAAGA TCATCTGCTG   481 CAGCTGGACA AATGATGGTC AGTACCTGGC GCTGGGGATG TTCAATGGGA TCATCAGCAT   541 ACGGAACAAA AATGGCGAGG AGAAAGTAAA GATCGAGCGG CCGGGGGGCT CCCTCTCGCC   601 AATATGGTCC ATCTGCTGGA ACCCTTCAAG AGAGGAACGT AATGACATCC TGGCTGTGGC   661 TGACTGGGGA CAGAAAGTTT CCTTCTACCA GCTGAGTGGA AAACAGATTG GAAAGGATCG   721 GGCACTGAAC TTTGACCCCT GCTGCATCAG CTACTTTACT AAAGGCGAGT ACATTTTGCT   781 GGGGGGTTCA GACAAGCAAG TATCTCTTTT CACCAAGGAT GGAGTGCGGC TTGGGACTGT   841 TGGGGAGCAG AACTCCTGGG TGTGGACGTG TCAAGCGAAA CCGGATTCCA ACTATGTGGT   901 GGTCGGCTGC CAGGACGGCA CCATTTCCCT TCTACCAGCT TATTTTCAGC ACAGTCCATG   961 GGCTTTACAA GGACCGCTAT GCCCTACAGG GATAGCATGA CTGACGTCAT TGTGCAGCAC  1021 CTGATCACTG AGCAGAAAGT TCGGATAATG CTAAGAGCTT GTCAGAGATT GCAATCTACA  1081 GAATCGATGC TATCACTGCT GGAAATCCTC ATCTATGGAG TTGTTATTTC AGGAGGAAAC 1141 TTATTCTCCA //
Primer: pCAGGS_R1 Region: beta-glonbinpA,insert 3' Sequence file: RDB15190_A7Fsb.seq
>D04646A1_A7Fs_1_pCAGGS_R1_A08_02_ABI24.ab1     1 NNNNNNNNNN NNNNCNCNNT TGNNNTGAAA NNAAATTTCC TTTATTAGCC AGAAGTCAGA    61 TGCTCAAGGG GCTTCATGAT GTCCCCATAA TTTTTGGCAG AGGGAAAAAG ATCCCAGTGG   121 TATTTGTGAG CCAGGGCATT GGCCACACCA GCCACCACCT TCTGATAGGC AGCCTGCACC   181 TGAGGAGTCG ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTCA   241 TGGGCCAGGG TCATCCTTGC ACCTGCGGCA GTAGGGGCAG CAGCCATGCT GAAGCACCAG   301 CAACTCATAG TCCTCAGAAT GGAACATCTG GAAGCAGGAG GGGCACATGG TAATGGAGGC   361 GTCAGGCAGC AGTGAGCGGA AGTATTGCCA CCTCAGGGGT GGGGGCCATC GCTTGATGAG   421 GACATCCCGG CGGCTCATGG AGCGCAGCAC CAGCCGGCTC ACCACCACTG GCACGAACTC   481 TGAGCCACCT TGCTCAAAGC TCAGCTTAGC TGTGAACGGG TCCTCATCTC CGATGGAGTC   541 CTTGGTCTCC ACTAGCCGCA GAATCTGGGA GCTGTTGTTT GCAATCTCTA GCTGTCTGTC   601 ATCCCGCTTG GGTCTCAGCA CCTCCAGGTC GATGAGGGAG ATGGCTTCTT CATCAGTGAT   661 CCCTTCCTCC AGGTAGAACT CAACCAGGTG TAGCACGTCG TANGAAGAGG CGGANAAGAN   721 GAAGGGCTGG CGGCAGTTGN TGCAGACGTT GCCCAGGTTG TTGAGCAGCG GGTTGTTGGT   781 GGAGCAGCGG TAGCACAAGG GCACCAACTN CTCACTGTCG TGNAANGGCT TGGCGCGGNT   841 GGTCANGGTA NCCAGCTCAA TGGNNTTTTG GAATCTGGNA NGGNNGTACA GGNCACGCAN   901 CTTGNCATNN NCGNGNCNGN NNNGCCNNNN NNNANNNNNG NNNNNGNNNN GNTNNNNNNA 961 NNNNNNNNNN //
Primer: EGFP-N Region: EGFP, CAG pro Sequence file: RDB15190_A7Fsc.seq
>D04646A1_A7Fs_1_EGFP-N_A12_03_ABI24.ab1     1 GCACCCCGGG CGACGCTCCT CGCCCTTGCT CACCATGGTG GCGACCGGTA ATTTGCCAAA    61 ATGATGAGAC AGCACAATAA CCAGCACGTT GCCCAGGAGC TGTAGGAAAA AGAAGAAGGC   121 ATGAACATGG TTAGCAGAGG CTAGAGCCGC CGGTCACACG CCAGAAGCCG AACCCCGCCC   181 TGCCCCGTCC CCCCCGAAGG CAGCCGTCCC CCCGCGGACA GCCCCGAGGC TGGAGAGGGA   241 GAAGGGGACG GCGGCGCGGC GACGCACGAA GGCCCTCCCC GCCCATTTCC TTCCTGCCGG   301 CGCCGCACCG CTTCGCCCCG CGCCCGCTAG AGGGGGTGCG GCGGCGCCTC CCAGATTTCG   361 GCTCCGCACA GATTTGGGAC AAAGGAAGTC CCTGCGCCCT CTCGCACGAT TACCATAAAA   421 GGCAATGGCT GCGGCTCGCC GCGCCTCGAC AGCCGCCGGC GCTCCGGGGG CCGCCGCGCC   481 CCTCCCCCGA GCCCTCCCCG GCCCGAGGCG GCCCCGCCCC GCCCGGCACC CCCACCTGCC   541 GCCACCCCCC GCCCGGCACG GCGAGCCCCG CGCCACGCCC CGTACGGAGC CCCGCACCCG 601 AAGCCGGGCC GTGCTCAGCA ACTCGGGGAG GGGGGTGCAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl