Resource data sheet
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pCAG/hArf4(WT)-EGFP (#RDB15358)

Expression vector of human ARF4, wild type.

Alternative name W68
Clone info. Expression vector of human ADP-ribosylation factor4 (ARF4), wild type, tagged with EGFP at C-ter, CAG promoter.
Comment Expression was confirmed by the depositor with immunoblotting
Vector backbone pCAGGS (plasmid)
Selectable markers Amp^r.
Gene/insert name human ARF4 cDNA
Depositor|Developer Nakayama, Kazuhisa |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nakai, W. et al. Mol. Biol. Cell, 24 (16): 2570-2581, 2013). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakai, W. et al. Mol. Biol. Cell, 24 (16): 2570-2581, 2013)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15358 pCAG/hArf4(WT)-EGFP Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pCAG/hArf4(WT)-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15358).

Reference section:

Nakai, W., Kondo, Y., Saitoh, A., Naito, T., Nakayama, K., Shin, H.W., ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Mol. Biol. Cell 24 (16): 2570-2581 (2013). PMID 23783033. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Nakai, W., ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Mol. Biol. Cell 24 (16): 2570-2581 (2013). PMID 23783033. [link to RRC of NBRP]