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pcDNA3/hArf6(T27N)-HA (#RDB15351)

Expression vector of human ARF6, inactivating mutant T27N.

Alternative name W43
Clone info. Expression vector of human ADP-ribosylation factor6 (ARF6), inactivating mutant T27N, tagged with HA epitope at C-ter.
Comment Expression was confirmed by the depositor with immunostaining. This clone contains a cDNA fragment corresponding to the 558nt to 1082nt of NM_001663.4 . Known differences : G to A substitution at 626nt (Ala, synonym) ; A to G substitution at 641nt (Thr, synonym).
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cels).
Gene/insert name human ARF6 cDNA
Depositor|Developer Nakayama, Kazuhisa |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makyio, H. et al. EMBO. J., 31 (11): 2590-2603, 2012). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makyio, H. et al. EMBO. J., 31 (11): 2590-2603, 2012)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。

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RDB15351 pcDNA3/hArf6(T27N)-HA DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3/hArf6(T27N)-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15351).

Reference section:

Makyio, H., Ohgi, M., Takei, T., Takahashi, S., Takatsu, H., Katoh, Y., Hanai, A., Ueda, T., Kanaho, Y., Xie, Y., Shin, H.W., Kamikubo, H., Kataoka, M., Kawasaki, M., Kato, R., Wakatsuki, S., Nakayama, K., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15351_A7I5p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, insert (hArf6 (T27N)), HA
Sequence file: RDB15351_A7I5a.seq check
>D03588B6_A7I5_1_CMV-Forward_D04_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTTGN NNNNNNGGGC GGTANGCGTG TACGGTGGGA NGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTTGGTAC CGAGCTCGGA TCTGCCACCA TGGGGAAGGT
  181 GCTATCCAAA ATCTTCGGGA ACAAGGAAAT GCGGATCCTC ATGTTGGGCC TGGACGCAGC
  241 CGGCAAGAAC ACGATCCTGT ACAAGTTGAA GCTGGGCCAG TCGGTGACCA CCATTCCCAC
  301 TGTGGGTTTC AACGTGGAGA CGGTGACTTA CAAAAATGTC AAGTTCAACG TATGGGATGT
  361 GGGCGGCCAG GACAAGATCC GGCCGCTCTG GCGGCATTAC TACACTGGGA CCCAAGGTCT
  421 CATCTTCGTA GTGGACTGCG CCGACCGCGA CCGCATCGAT GAGGCTCGCC AGGAGCTGCA
  481 CCGCATTATC AATGACCGGG AGATGAGGGA CGCCATAATC CTCATCTTCG CCAACAAGCA
  541 GGACCTGCCC GATGCCATGA AACCCCACGA GATCCAGGAG AAACTGGGCC TGACCCGGAT
  601 TCGGGACAGG AACTGGTATG TGCAGCCCTC CTGTGCCACC TCAGGGGACG GACTCTATGA
  661 GGGGCTCACA TGGTTAACCT CTAACTACAA ATCTCTCGAG TATCCATACG ATGTACCAGA
  721 CTACGCAGAG ATCCACTAGT TCTAGANGGC CCTATTCTAT AGTGTCACCT AAATGCTAGA
  781 GCTCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC
  841 CCCGTGCCTT NCTNGANCCT GGAANGTGCC NNNCCCNCNG TCCTTTCCTA ANAAAATGAN
  901 GAAATTGNAT CNCATTGTCT GANTANGNGN CNTTCTANTC TNGGGGGGNN GGGNNGGGGG
961 NNNNANNNNN NNGGGGNNNN NNNNGGNANN NNNNNNNN
//
Primer: SV40pro_F (Pr0110)
Region: NeoR
Sequence file: RDB15351_A7I5b.seq check
>D03588B6_A7I5_1_SV40pro_F_F04_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNAN NNNTGNNGNN GCTTTTTTGG AGGCCTAGGC TTTTGCNAAA
   61 AGCTCCCGGG NGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA GGNTCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GANCGCTATC
  781 ANGACATAGC GTTGGCTACN CGTGATATTG CTGAAGANCT TGGCGGCGAA TGNNCTGACN
  841 GCTTCCTCGN GCTTTACGGT ATCGCCGCNC CCGATTCGCA NCGCATCGCC TTCNATCGCC
  901 NTCTTGACNA NTNCNTCTGA NCGGGNNTCN GGGGGNTCNN AANGNNNGNN NNANNNACNN
  961 CNNANCNTGN CNNNCNCNNN NNTNNNANNN NNNCNNNCNN NCNNTNNNNN NNANNNNNNG
1021 NNNTNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702. [link to RRC of NBRP]