RDB15349 - pcDNA3/hArf6(WT)-HA

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pcDNA3/hArf6(WT)-HA

Expression vector of human ARF6, wild type.

Catalog number	RDB15349
Resource name	pcDNA3/hArf6(WT)-HA
Alternative name	W42
Clone info.	Expression vector of human ADP-ribosylation factor6 (ARF6), wild type, tagged with HA epitope at C-ter.
Comment	Expression was confirmed by the depositor with immunostaining.
Vector backbone	pcDNA3 (plasmid)
Size of vector backbone	5.4 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cels).
Gene/insert name	human ARF6 cDNA
Depositor\|Developer	Nakayama, Kazuhisa \|

Other clones in our bank

human ARF6

External Database
human ARF6

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makyio, H. et al. EMBO. J., 31 (11): 2590-2603, 2012). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makyio, H. et al. EMBO. J., 31 (11): 2590-2603, 2012). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makyio, H. et al. EMBO. J., 31 (11): 2590-2603, 2012)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15349	pcDNA3/hArf6(WT)-HA	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original	Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15349_A7I5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro, insert (hArf6(WT)), HA Sequence file: RDB15349_A7I5a.seq
>D03588B4_A7I5_2_CMV_Forward_A03_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN GCNNNGGGCG GTANGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA 121 CTCACTATAG GGAGACCCAA GCTTGGTACC GAGCTCGGAT CTGCCACCAT GGGGAAGGTG 181 CTATCCAAAA TCTTCGGGAA CAAGGAAATG CGGATCCTCA TGTTGGGCCT GGACGCGGCC 241 GGCAAGACAA CAATCCTGTA CAAGTTGAAG CTGGGCCAGT CGGTGACCAC CATTCCCACT 301 GTGGGTTTCA ACGTGGAGAC GGTGACTTAC AAAAATGTCA AGTTCAACGT ATGGGATGTG 361 GGCGGCCAGG ACAAGATCCG GCCGCTCTGG CGGCATTACT ACACTGGGAC CCAAGGTCTC 421 ATCTTCGTAG TGGACTGCGC CGACCGCGAC CGCATCGATG AGGCTCGCCA GGAGCTGCAC 481 CGCATTATCA ATGACCGGGA GATGAGGGAC GCCATAATCC TCATCTTCGC CAACAAGCAG 541 GACCTGCCCG ATGCCATGAA ACCCCACGAG ATCCAGGAGA AACTGGGCCT GACCCGGATT 601 CGGGACAGGA ACTGGTATGT GCAGCCCTCC TGTGCCACCT CAGGGGACGG ACTCTATGAG 661 GGGCTCACAT GGTTAACCTC TAACTACAAA TCTCTCGAGT ATCCATACGA TGTACCAGAC 721 TACGCAGANA TCCACTAGTT CTAGAGGGCC CTATTCTATA GTGTCACCTA AATGCTAGAG 781 CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC 841 CCCNNGCCTT CCTTGACCCT NGAANGTGCC ACTNCNNCTN NNCTTTCCTA ATAAAATGAN 901 GAAATTGCAT CGNNNNNNCT NNNNNNNNNN //
Primer: SV40pro_F Region: NeoR Sequence file: RDB15349_A7I5b.seq
>D03588B4-A7I5_2_SV40pro_F_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNTNGTGA NGANGNTTTT TTGGAGGNCT AGGCTTTTGC 61 AAAAAGCTCC CGNNNNGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG 121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA 181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT 241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT 301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG 361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT 421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC 481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC 541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA 601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG 661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT 721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG 781 CTATCANGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA NAGCTTGGCG GCGANNGNGC 841 TGACCGCTTC CTCNTGCTTT ACGGNATCGC CNCTCCCGNN NCGCAGCGCA TCGCCTTCTA 901 TCGCCTTCTT GNCNANTNCT NCTGANCGGG NNTCTGGGGN NNNNAAATGN NCNNNCNANC 961 NANGCCNNNN CCTGNCNNNC NNNANANNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702.

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl