RDB15191 - pCAG2-mCherry-C1-IFT122

Expression vector of human intraflagellar transport 122 (IFT122), tagged with N-terminal mCherry, CAG promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes requires a separate license from the DEPOSITOR, prior to distribution.
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内)

[open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。本件リソースを学術研究以外に利用する場合は、事前に寄託者との間で、別途使用許諾にかかる契約の締結が必要である。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15191	pCAG2-mCherry-C1-IFT122	DNA solution

original	Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497.

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: mRFP1-inner1 Region: mCherry, insert 5' Sequence file: RDB15191_A7Fsa.seq
>D04646A2_A7Fs_2_mRFP1-inner1_E07_13_ABI24.ab1 1 GAACGGCGTT ATGACGGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA 61 GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT 121 GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT 181 CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGAGGTCA AGACCACCTA 241 CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC GTCAACATCA AGTTGGACAT 301 CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC GAACGCGCCG AGGGCCGCCA 361 CTCCACCGGC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCTCGAG CTCAAGCTTC 421 GAATTCTATG AGGGCCGTGT TGACGTGGAG AGATAAAGCC GAGCACTGTA TAAATGACAT 481 CGCATTTAAG CCTGATGGAA CTCAACTGAT TTTGGCTGCC GGAAGCAGAT TACTGGTTTA 541 TGACACCTCT GATGGCACCT TACTTCAGCC CCTCAAGGGA CACAAAGACA CTGTGTACTG 601 TGTGGCATAT GCGAAGGATG GCAAGCGCTT TGCTTCTGGA TCAGCTGACA AAAGCGTTAT 661 TATCTGGACA TCAAAACTGG AAGGCATTCT GAAGTACACG CACAATGATG CTATACAATG 721 TGTCTCCTAC AATCCTATTA CTCATCAACT GGCATCTTGT TCCTCCAGTG ACTTTGGGTT 781 GTGGTCTCCT GAACAGAAGT CTGTCTCCAA ACACAAATCA AGCAGCAAGA TCATCTGCTG 841 CAGCTGGACA AATGATGGTC AGTACCTGGC GCTGGGGATG TTCAATGGGA TCATCAGCAT 901 ACGGAACAAA AATGGCGAGG AGAAAGTAAA GATCGAGCGG CCGGGGGGCT CCCTCTCGCC 961 ATATGGGTCC ATCTGCTGGA CCCTTTCAGA GAGGACGTAA TGACATCCTT GCTTGTTGCT 1021 GACTGGGGAC AAGAAAGTTC GTCCTACAGC TGATGAAAAC AGATGGAAAA GGATCCGGCA 1081 CTGGACTTTG ACCTGCTGCA TCAGCTACTT ACTAAGGCGA GTACATTTGC CTGAG //
Primer: pCAGGS_R1 Region: beta-globinpA,insert 3' Sequence file: RDB15191_A7Fsb.seq
>D04646A2_A7Fs_2_pCAGGS_R1_G01_07_ABI08.ab1 1 NNNNNNNNNN NNNNCNCNCT ATTGCNNTGN NAATAAATTT CCTTTATTAG CCAGAAGTCA 61 GATGCTCAAG GGGCTTCATG ATGTCCCCAT AATTTTTGGC AGAGGGAAAA AGATCCCAGT 121 GGTATTTGTG AGCCAGGGCA TTGGCCACAC CAGCCACCAC CTTCTGATAG GCAGCCTGCA 181 CCTGAGGAGT CTATCAGTTA TCTAGATCCG GTGGATCCCG GGCCCGCGGT ACCGTCGACT 241 CATGGGCCAG GGTCATCCTT GCACCTGCGG CAGTAGGGGC AGCAGCCATG CTGAAGCACC 301 AGCAACTCAT AGTCCTCAGA ATGGAACATC TGGAAGCAGG AGGGGCACAT GGTAATGGAG 361 GCGTCAGGCA GCAGTGAGCG GAAGTATTGC CACCTCAGGG GTGGGGGCCA TCGCTTGATG 421 AGGACATCCC GGCGGCTCAT GGAGCGCAGC ACCAGCCGGC TCACCACCAC TGGCACGAAC 481 TCTGAGCCAC CTTGCTCAAA GCTCAGCTTA GCTGTGAACG GGTCCTCATC TCCGATGGAG 541 TCCTTGGTCT CCACTAGCCG CAGAATCTGG GAGCTGTTGT TTGCAATCTC TAGCTGTCTG 601 TCATCCCGCT TGGGTCTCAG CACCTCCAGG TCGATGAGGG AGATGGCTTC TTCATCAGTG 661 ATCCCTTCCT CCAGGTAGAA CTCAACCAGG TGTAGCACGT CGTANGAAGA GGCGGANAAG 721 ATGAAGGGCT GGCGGCAGTT GATGCAGACG TTGCCCAGGT TGTTGAGCAG CGGGTTGTTG 781 GTGGAGCAGC GGTAGCACAA GGGCACCAAC TCCTCACTGT CGTGNAANGG CTTGGCGCGG 841 ANGGTCAGGG TACCCAGCTC AATGNANTTT TNGAATCTGG CANGGNNGTN CANGCCACGC 901 ANCTNGNCAT ANGCGNGNNN GGNCAGNNTG NNNNCNNCNA NGGCCTNNNN NNGCTNNNNN 961 NANNNTGAAN ANTATTTTCN NNTNNNNNNN NNCNNNNGGG NNNNNNNTNN GNNNNNNNNN 1021 N //
Primer: Red-N_R Region: mCherry, CAG pro Sequence file: RDB15191_A7Fsc.seq
>D04646A2_A7Fs_2_Red-N_R_E05_05_ABI08.ab1 1 GACTTAGGGG GCACCTTTGA AGCGCATGAC TCCTTGATGA TGGCCATGTT ATCCTCCTCG 61 CCCTTGCTCA CCATGGTGGC GCTAGCATTT GCCAAAATGA TGAGACAGCA CAATAACCAG 121 CACGTTGCCC AGGAGCTGTA GGAAAAAGAA GAAGGCATGA ACATGGTTAG CAGAGGCTAG 181 AGCCGCCGGT CACACGCCAG AAGCCGAACC CCGCCCTGCC CCGTCCCCCC CGAAGGCAGC 241 CGTCCCCCCG CGGACAGCCC CGAGGCTGGA GAGGGAGAAG GGGACGGCGG CGCGGCGACG 301 CACGAAGGCC CTCCCCGCCC ATTTCCTTCC TGCCGGCGCC GCACCGCTTC GCCCCGCGCC 361 CGCTAGAGGG GGTGCGGCGG CGCCTCCCAG ATTTCGGCTC CGCACAGATT TGGGACAAAG 421 GAAGTCCCTG CGCCCTCTCG CACGATTACC ATAAAAGGCA ATGGCTGCGG CTCGCCGCGC 481 CTCGACAGCC GCCGGCGCTC CGGGGGCCGC CGCGCCCCTC CCCCGAGCCC TCCCCGGCCC 541 GAGGCGGCCC CGCCCCGCCC GGCACCCCCA CCTGCCGCCA CCCCCCGCCC GGCACGGCGA 601 GCCCCGCGCC ACGCCCCGTA CGGAGCCCCG CACCCGAAGC CGGGCCGTGC TCAGCAACTC 661 GGGGAGGGGG GTGCAGGGG //