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pCAG2-mCherry-C1-IFT122

Expression vector of human intraflagellar transport 122 (IFT122), tagged with N-terminal mCherry, CAG promoter.

Catalog number RDB15191
Resource name pCAG2-mCherry-C1-IFT122
Clone info. Expression vector of human intraflagellar transport 122 (IFT122), tagged with N-terminal mCherry, CAG promoter.
Expression was confirmed by the depositor with the visible immunoprecipitation assay. Insert fragment was derived from IRAL009C12 (HGY083660).
Vector backbone pCAG2-mCherry-C1 (plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name human IFT122 cDNA
Depositor|Developer Nakayama, Kazuhisa | Katoh, Yohei |
 
Other clones in our bank human IFT122 (NCBI Gene 55764) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-199, 1991). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes requires a separate license from the DEPOSITOR, prior to distribution.
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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RDB15191 pCAG2-mCherry-C1-IFT122 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15191_A7Fsp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mRFP1-inner1
Region: mCherry, insert 5'
Sequence file: RDB15191_A7Fsa.seq check
>D04646A2_A7Fs_2_mRFP1-inner1_E07_13_ABI24.ab1
    1 GAACGGCGTT ATGACGGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA
   61 GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT
  121 GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT
  181 CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGAGGTCA AGACCACCTA
  241 CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC GTCAACATCA AGTTGGACAT
  301 CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC GAACGCGCCG AGGGCCGCCA
  361 CTCCACCGGC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCTCGAG CTCAAGCTTC
  421 GAATTCTATG AGGGCCGTGT TGACGTGGAG AGATAAAGCC GAGCACTGTA TAAATGACAT
  481 CGCATTTAAG CCTGATGGAA CTCAACTGAT TTTGGCTGCC GGAAGCAGAT TACTGGTTTA
  541 TGACACCTCT GATGGCACCT TACTTCAGCC CCTCAAGGGA CACAAAGACA CTGTGTACTG
  601 TGTGGCATAT GCGAAGGATG GCAAGCGCTT TGCTTCTGGA TCAGCTGACA AAAGCGTTAT
  661 TATCTGGACA TCAAAACTGG AAGGCATTCT GAAGTACACG CACAATGATG CTATACAATG
  721 TGTCTCCTAC AATCCTATTA CTCATCAACT GGCATCTTGT TCCTCCAGTG ACTTTGGGTT
  781 GTGGTCTCCT GAACAGAAGT CTGTCTCCAA ACACAAATCA AGCAGCAAGA TCATCTGCTG
  841 CAGCTGGACA AATGATGGTC AGTACCTGGC GCTGGGGATG TTCAATGGGA TCATCAGCAT
  901 ACGGAACAAA AATGGCGAGG AGAAAGTAAA GATCGAGCGG CCGGGGGGCT CCCTCTCGCC
  961 ATATGGGTCC ATCTGCTGGA CCCTTTCAGA GAGGACGTAA TGACATCCTT GCTTGTTGCT
 1021 GACTGGGGAC AAGAAAGTTC GTCCTACAGC TGATGAAAAC AGATGGAAAA GGATCCGGCA
1081 CTGGACTTTG ACCTGCTGCA TCAGCTACTT ACTAAGGCGA GTACATTTGC CTGAG
//
Primer: pCAGGS_R1
Region: beta-globinpA,insert 3'
Sequence file: RDB15191_A7Fsb.seq check
>D04646A2_A7Fs_2_pCAGGS_R1_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNCNCNCT ATTGCNNTGN NAATAAATTT CCTTTATTAG CCAGAAGTCA
   61 GATGCTCAAG GGGCTTCATG ATGTCCCCAT AATTTTTGGC AGAGGGAAAA AGATCCCAGT
  121 GGTATTTGTG AGCCAGGGCA TTGGCCACAC CAGCCACCAC CTTCTGATAG GCAGCCTGCA
  181 CCTGAGGAGT CTATCAGTTA TCTAGATCCG GTGGATCCCG GGCCCGCGGT ACCGTCGACT
  241 CATGGGCCAG GGTCATCCTT GCACCTGCGG CAGTAGGGGC AGCAGCCATG CTGAAGCACC
  301 AGCAACTCAT AGTCCTCAGA ATGGAACATC TGGAAGCAGG AGGGGCACAT GGTAATGGAG
  361 GCGTCAGGCA GCAGTGAGCG GAAGTATTGC CACCTCAGGG GTGGGGGCCA TCGCTTGATG
  421 AGGACATCCC GGCGGCTCAT GGAGCGCAGC ACCAGCCGGC TCACCACCAC TGGCACGAAC
  481 TCTGAGCCAC CTTGCTCAAA GCTCAGCTTA GCTGTGAACG GGTCCTCATC TCCGATGGAG
  541 TCCTTGGTCT CCACTAGCCG CAGAATCTGG GAGCTGTTGT TTGCAATCTC TAGCTGTCTG
  601 TCATCCCGCT TGGGTCTCAG CACCTCCAGG TCGATGAGGG AGATGGCTTC TTCATCAGTG
  661 ATCCCTTCCT CCAGGTAGAA CTCAACCAGG TGTAGCACGT CGTANGAAGA GGCGGANAAG
  721 ATGAAGGGCT GGCGGCAGTT GATGCAGACG TTGCCCAGGT TGTTGAGCAG CGGGTTGTTG
  781 GTGGAGCAGC GGTAGCACAA GGGCACCAAC TCCTCACTGT CGTGNAANGG CTTGGCGCGG
  841 ANGGTCAGGG TACCCAGCTC AATGNANTTT TNGAATCTGG CANGGNNGTN CANGCCACGC
  901 ANCTNGNCAT ANGCGNGNNN GGNCAGNNTG NNNNCNNCNA NGGCCTNNNN NNGCTNNNNN
  961 NANNNTGAAN ANTATTTTCN NNTNNNNNNN NNCNNNNGGG NNNNNNNTNN GNNNNNNNNN
1021 N
//
Primer: Red-N_R
Region: mCherry, CAG pro
Sequence file: RDB15191_A7Fsc.seq check
>D04646A2_A7Fs_2_Red-N_R_E05_05_ABI08.ab1
    1 GACTTAGGGG GCACCTTTGA AGCGCATGAC TCCTTGATGA TGGCCATGTT ATCCTCCTCG
   61 CCCTTGCTCA CCATGGTGGC GCTAGCATTT GCCAAAATGA TGAGACAGCA CAATAACCAG
  121 CACGTTGCCC AGGAGCTGTA GGAAAAAGAA GAAGGCATGA ACATGGTTAG CAGAGGCTAG
  181 AGCCGCCGGT CACACGCCAG AAGCCGAACC CCGCCCTGCC CCGTCCCCCC CGAAGGCAGC
  241 CGTCCCCCCG CGGACAGCCC CGAGGCTGGA GAGGGAGAAG GGGACGGCGG CGCGGCGACG
  301 CACGAAGGCC CTCCCCGCCC ATTTCCTTCC TGCCGGCGCC GCACCGCTTC GCCCCGCGCC
  361 CGCTAGAGGG GGTGCGGCGG CGCCTCCCAG ATTTCGGCTC CGCACAGATT TGGGACAAAG
  421 GAAGTCCCTG CGCCCTCTCG CACGATTACC ATAAAAGGCA ATGGCTGCGG CTCGCCGCGC
  481 CTCGACAGCC GCCGGCGCTC CGGGGGCCGC CGCGCCCCTC CCCCGAGCCC TCCCCGGCCC
  541 GAGGCGGCCC CGCCCCGCCC GGCACCCCCA CCTGCCGCCA CCCCCCGCCC GGCACGGCGA
  601 GCCCCGCGCC ACGCCCCGTA CGGAGCCCCG CACCCGAAGC CGGGCCGTGC TCAGCAACTC
661 GGGGAGGGGG GTGCAGGGG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497.

2020.11.04

GNP_filter3_RDBDEP_html_201104.pl