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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG2-mCherry-C1-IFT122

Expression vector of human intraflagellar transport 122 (IFT122), tagged with N-terminal mCherry, CAG promoter.

Catalog number RDB15191
Resource name pCAG2-mCherry-C1-IFT122
Clone info. Expression vector of human intraflagellar transport 122 (IFT122), tagged with N-terminal mCherry, CAG promoter.
Comment Expression was confirmed by the depositor with the visible immunoprecipitation assay. Insert fragment was derived from IRAL009C12 (HGY083660).
Vector backbone pCAG2-mCherry-C1 (plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name human IFT122 cDNA
Depositor|Developer Nakayama, Kazuhisa | Katoh, Yohei |
Other clones in our bank

External Database
human IFT122

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes requires a separate license from the DEPOSITOR, prior to distribution.
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。本件リソースを学術研究以外に利用する場合は、事前に寄託者との間で、別途使用許諾にかかる契約の締結が必要である。
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15191 pCAG2-mCherry-C1-IFT122 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15191_A7Fsp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: mRFP1-inner1
          Region: mCherry, insert 5'
          Sequence file: RDB15191_A7Fsa.seq check
          >D04646A2_A7Fs_2_mRFP1-inner1_E07_13_ABI24.ab1
              1 GAACGGCGTT ATGACGGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA
             61 GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT
            121 GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT
            181 CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGAGGTCA AGACCACCTA
            241 CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC GTCAACATCA AGTTGGACAT
            301 CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC GAACGCGCCG AGGGCCGCCA
            361 CTCCACCGGC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCTCGAG CTCAAGCTTC
            421 GAATTCTATG AGGGCCGTGT TGACGTGGAG AGATAAAGCC GAGCACTGTA TAAATGACAT
            481 CGCATTTAAG CCTGATGGAA CTCAACTGAT TTTGGCTGCC GGAAGCAGAT TACTGGTTTA
            541 TGACACCTCT GATGGCACCT TACTTCAGCC CCTCAAGGGA CACAAAGACA CTGTGTACTG
            601 TGTGGCATAT GCGAAGGATG GCAAGCGCTT TGCTTCTGGA TCAGCTGACA AAAGCGTTAT
            661 TATCTGGACA TCAAAACTGG AAGGCATTCT GAAGTACACG CACAATGATG CTATACAATG
            721 TGTCTCCTAC AATCCTATTA CTCATCAACT GGCATCTTGT TCCTCCAGTG ACTTTGGGTT
            781 GTGGTCTCCT GAACAGAAGT CTGTCTCCAA ACACAAATCA AGCAGCAAGA TCATCTGCTG
            841 CAGCTGGACA AATGATGGTC AGTACCTGGC GCTGGGGATG TTCAATGGGA TCATCAGCAT
            901 ACGGAACAAA AATGGCGAGG AGAAAGTAAA GATCGAGCGG CCGGGGGGCT CCCTCTCGCC
            961 ATATGGGTCC ATCTGCTGGA CCCTTTCAGA GAGGACGTAA TGACATCCTT GCTTGTTGCT
           1021 GACTGGGGAC AAGAAAGTTC GTCCTACAGC TGATGAAAAC AGATGGAAAA GGATCCGGCA
          1081 CTGGACTTTG ACCTGCTGCA TCAGCTACTT ACTAAGGCGA GTACATTTGC CTGAG
          //
          Primer: pCAGGS_R1
          Region: beta-globinpA,insert 3'
          Sequence file: RDB15191_A7Fsb.seq check
          >D04646A2_A7Fs_2_pCAGGS_R1_G01_07_ABI08.ab1
              1 NNNNNNNNNN NNNNCNCNCT ATTGCNNTGN NAATAAATTT CCTTTATTAG CCAGAAGTCA
             61 GATGCTCAAG GGGCTTCATG ATGTCCCCAT AATTTTTGGC AGAGGGAAAA AGATCCCAGT
            121 GGTATTTGTG AGCCAGGGCA TTGGCCACAC CAGCCACCAC CTTCTGATAG GCAGCCTGCA
            181 CCTGAGGAGT CTATCAGTTA TCTAGATCCG GTGGATCCCG GGCCCGCGGT ACCGTCGACT
            241 CATGGGCCAG GGTCATCCTT GCACCTGCGG CAGTAGGGGC AGCAGCCATG CTGAAGCACC
            301 AGCAACTCAT AGTCCTCAGA ATGGAACATC TGGAAGCAGG AGGGGCACAT GGTAATGGAG
            361 GCGTCAGGCA GCAGTGAGCG GAAGTATTGC CACCTCAGGG GTGGGGGCCA TCGCTTGATG
            421 AGGACATCCC GGCGGCTCAT GGAGCGCAGC ACCAGCCGGC TCACCACCAC TGGCACGAAC
            481 TCTGAGCCAC CTTGCTCAAA GCTCAGCTTA GCTGTGAACG GGTCCTCATC TCCGATGGAG
            541 TCCTTGGTCT CCACTAGCCG CAGAATCTGG GAGCTGTTGT TTGCAATCTC TAGCTGTCTG
            601 TCATCCCGCT TGGGTCTCAG CACCTCCAGG TCGATGAGGG AGATGGCTTC TTCATCAGTG
            661 ATCCCTTCCT CCAGGTAGAA CTCAACCAGG TGTAGCACGT CGTANGAAGA GGCGGANAAG
            721 ATGAAGGGCT GGCGGCAGTT GATGCAGACG TTGCCCAGGT TGTTGAGCAG CGGGTTGTTG
            781 GTGGAGCAGC GGTAGCACAA GGGCACCAAC TCCTCACTGT CGTGNAANGG CTTGGCGCGG
            841 ANGGTCAGGG TACCCAGCTC AATGNANTTT TNGAATCTGG CANGGNNGTN CANGCCACGC
            901 ANCTNGNCAT ANGCGNGNNN GGNCAGNNTG NNNNCNNCNA NGGCCTNNNN NNGCTNNNNN
            961 NANNNTGAAN ANTATTTTCN NNTNNNNNNN NNCNNNNGGG NNNNNNNTNN GNNNNNNNNN
          1021 N
          //
          Primer: Red-N_R
          Region: mCherry, CAG pro
          Sequence file: RDB15191_A7Fsc.seq check
          >D04646A2_A7Fs_2_Red-N_R_E05_05_ABI08.ab1
              1 GACTTAGGGG GCACCTTTGA AGCGCATGAC TCCTTGATGA TGGCCATGTT ATCCTCCTCG
             61 CCCTTGCTCA CCATGGTGGC GCTAGCATTT GCCAAAATGA TGAGACAGCA CAATAACCAG
            121 CACGTTGCCC AGGAGCTGTA GGAAAAAGAA GAAGGCATGA ACATGGTTAG CAGAGGCTAG
            181 AGCCGCCGGT CACACGCCAG AAGCCGAACC CCGCCCTGCC CCGTCCCCCC CGAAGGCAGC
            241 CGTCCCCCCG CGGACAGCCC CGAGGCTGGA GAGGGAGAAG GGGACGGCGG CGCGGCGACG
            301 CACGAAGGCC CTCCCCGCCC ATTTCCTTCC TGCCGGCGCC GCACCGCTTC GCCCCGCGCC
            361 CGCTAGAGGG GGTGCGGCGG CGCCTCCCAG ATTTCGGCTC CGCACAGATT TGGGACAAAG
            421 GAAGTCCCTG CGCCCTCTCG CACGATTACC ATAAAAGGCA ATGGCTGCGG CTCGCCGCGC
            481 CTCGACAGCC GCCGGCGCTC CGGGGGCCGC CGCGCCCCTC CCCCGAGCCC TCCCCGGCCC
            541 GAGGCGGCCC CGCCCCGCCC GGCACCCCCA CCTGCCGCCA CCCCCCGCCC GGCACGGCGA
            601 GCCCCGCGCC ACGCCCCGTA CGGAGCCCCG CACCCGAAGC CGGGCCGTGC TCAGCAACTC
          661 GGGGAGGGGG GTGCAGGGG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl