Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCAG2-EGFP-C1-IFT140

Expression vector of human intraflagellar transport 140 (IFT140), tagged with N-terminal EGFP, CAG promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes requires a separate license from the DEPOSITOR, prior to distribution. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。本件リソースを学術研究以外に利用する場合は、事前に寄託者との間で、別途使用許諾にかかる契約の締結が必要である。 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15192_A7Fsp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C (Pr0025) Region: EGFP, insert 5' Sequence file: RDB15192_A7Fsa.seq
>D04646A3_A7Fs_1_EGFP-C_B12_06_ABI24.ab1     1 CAGGGAATCT CGGCATGGAC GAGCTGTACA AGTCCGGACT CAGATCTCGA GCTCAAGCTT    61 CGAATTCTAT GGCCCTCTAT TATGACCACC AGATAGAAGC CCCGGATGCA GCAGGGTCAC   121 CCTCATTTAT CAGCTGGCAC CCTGTCCATC CATTCTTGGC AGTTGCTTAC ATCAGCACAA   181 CCTCAACAGG CAGCGTGGAT ATTTACCTGG AGCAAGGGGA GTGCGTGCCA GATACACACG   241 TCGAGAGGCC GTTCCGGGTT GCTTCCCTGT GCTGGCACCC GACGCGGCTG GTGCTGGCTG   301 TGGGCTGGGA GACTGGAGAA GTGACGGTGT TTAACAAGCA GGACAAGGAG CAGCACACGA   361 TGCCCCTGAC ACACACAGCC GACATCACCG TGCTCCGTTG GAGCCCCAGT GGAAACTGCC   421 TGCTGTCTGG GGACAGGCTT GGTGTCTTGC TCTTGTGGAG GTTGGACCAA AGGGGCCGAG   481 TGCAAGGGAC GCCTCTGCTG AAACACGAGT ATGGGAAACA CCTCACGCAC TGCATCTTCC   541 GGCTCCCCCC TCCTGGCGAG GACCTGGTTC AGTTGGCAAA GGCAGCTGTG AGCGGTGATG   601 AGAAAGCCCT GGACATGTTT AACTGGAAGA AGAGCAGTTC TGGAAGTTTG CTGAAGATGG   661 GGTCTCACGA GGGGCTGTTG TTCTTTGTCA GTCTGATGGA CGGGACAGTG CACTATGTGG   721 ATGAGAAGGG CAAGACCACT CAGGTGGTGT CCGCAGACAG CACGATTCAG ATGCTGTTCT   781 ACATGGAGAA GAGGGAGGCA CTGGTGGTGG TCACAGAGAA CCTCCGGCTG TCCCTGTACA   841 CGGTGCCTCC TGAGGGCAAA GCAGAAGAAG TGATGAAGGT CAAGCTGAGC GGGAAAACCG   901 GCCGCCGGGC AGACATCGCT TTGATTGAAA GGCAGCCTTC TCGTGATGGC CGTCGGGGAG   961 GCTGCCCTCA GATTCTGGGA CATAGAACGA GGGAGAGAAT TATATACTGA GTTCCAGATG  1021 AGAAGTTTGG CTTTGAGAAG GAGAGGATTA TTGAACTTGT TGTGTGTTAC TGTAAGTCAA  1081 CGGTTCTTTC TGACGCTGTA CGACCGAAGG CCGAGTTAGC CCATGGGTGG GAGGGAAAAA 1141 GT //
Primer: pCA-bgter (Pr0066) Region: beta-glonbinpA,insert 3' Sequence file: RDB15192_A7Fsb.seq
>D04646A3_A7Fs_1_pCAGGS_R1_E08_14_ABI24.ab1     1 NNNNNNNNNN NNNNCNCTNT TGNNNTGNAA NNAAATTTCC TTTATTAGCC AGAAGTCAGA    61 TGCTCAAGGG GCTTCATGAT GTCCCCATAA TTTTTGGCAG AGGGAAAAAG ATCCCAGTGG   121 TATTTGTGAG CCAGGGCATT GGCCACACCA GCCACCACCT TCTGATAGGC AGCCTGCACC   181 TGAGGAGTCG ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTCA   241 GGGGTCGTCA TCTGCCTCTT CCACCACCTC CTCGTCCAGC TCCCTGGCGT CCTCCATGCT   301 GTTGTGGCGG ACCTGCTCGG GGACGGTGCG TGGCAGTGGG AGACCCAGCC CCCGGTGCAC   361 GGCGTCCACG GCCTGCGGGC TCACGTAGTA GGACATGTTG GCCAAGGGAA GCCGCCGCCG   421 CATCTCCTCC AGGAATCTGT AGGCCGTCTG GTATTCCTCC TTCCGCACGT AGTGCTCCAC   481 CAGGAAGCCA TAGACGTCCC CGATGCGGAT GGTGCTGTCC AGGTCTGGTT CCTCCAGGAG   541 CAGCTCACAC TGCTTGATGG ACTCCTTGGG GTCCTCTGTG TACGTCCTGC GGGCCTGGAT   601 GAACCTCTTC ACCAGTGCCA TCCTGCTCTG CAGCTGCGCC AGCCTGGTCT CCTGGTCCAG   661 GGGGCTCTTG GCCTTGGCCT TGGCCAGGCA CTTGTAGGCC TCGGTCAGCG CCCCGTGGGC   721 TTTGTCGTAG TTCTGGTATT CATCAATCTC CACCTGGGCA CAAGCGTCNT AAAAGCCAGC   781 CAGGANGTCC AGGGCCCGCC CCTTGGTGTA GAAGCCGATG ANGTTCTTCA TGATCTCCGG   841 CTCCTTCCGC CAGTCCAGNN NNTGCAGGTA GTTAGCAGCC ATGATGTANA TTTCCTTCTG   901 NCNNGNNACG CTCGCGAANA ACGNNNNTTT CTCCNNGNCT CCNGNNTTGA NCANCGCCNN 961 CNTNGNNNNT CANCTNNNTN NNNNGNNNNN NNNNN //
Primer: EGFP-N (Pr0027) Region: EGFP, CAG pro Sequence file: RDB15192_A7Fsc.seq
>D04646A3_A7Fs_1_EGFP-N_C08_08_ABI24.ab1     1 GGCCCCCGGG GGGACGCTCC TCGCCCTTGC TCACCATGGT GGCGACCGGT AATTTGCCAA    61 AATGATGAGA CAGCACAATA ACCAGCACGT TGCCCAGGAG CTGTAGGAAA AAGAAGAAGG   121 CATGAACATG GTTAGCAGAG GCTAGAGCCG CCGGTCACAC GCCAGAAGCC GAACCCCGCC   181 CTGCCCCGTC CCCCCCGAAG GCAGCCGTCC CCCCGCGGAC AGCCCCGAGG CTGGAGAGGG   241 AGAAGGGGAC GGCGGCGCGG CGACGCACGA AGGCCCTCCC CGCCCATTTC CTTCCTGCCG   301 GCGCCGCACC GCTTCGCCCC GCGCCCGCTA GAGGGGGTGC GGCGGCGCCT CCCAGATTTC   361 GGCTCCGCAC AGATTTGGGA CAAAGGAAGT CCCTGCGCCC TCTCGCACGA TTACCATAAA   421 AGGCAATGGC TGCGGCTCGC CGCGCCTCGA CAGCCGCCGG CGCTCCGGGG GCCGCCGCGC   481 CCCTCCCCCG AGCCCTCCCC GGCCCGAGGC GGCCCCGCCC CGCCCGGCAC CCCCACCTGC   541 CGCCACCCCC CGCCCGGCAC GGCGAGCCCC GCGCCACGCC CCGTACGGAG CCCCGCACCC 601 GAAGCCGGGC CGTGCTCAGC AACTCGGGGA GGGGGGTGCA G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl