RDB15193 - pCAG2-mCherry-C1-IFT140

Expression vector of human intraflagellar transport 140 (IFT140), tagged with N-terminal mCherry, CAG promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes requires a separate license from the DEPOSITOR, prior to distribution.
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内)

[open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017; Niwa, H., Gene 108 (2), 193-200, 1991)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。本件リソースを学術研究以外に利用する場合は、事前に寄託者との間で、別途使用許諾にかかる契約の締結が必要である。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15193	pCAG2-mCherry-C1-IFT140	DNA solution

original	Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497.

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: mRFP1-inner1 Region: mCherry, insert 5' Sequence file: RDB15193_A7Fsa.seq
>D04646A4_A7Fs_2_mRFP1-inner1_E12_15_ABI24.ab1 1 CGCGGGCGGG CGGACGTGAC CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG 61 TGAAGCTGCG CGGCACCAAC TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACCATGG 121 GCTGGGAGGC CTCCTCCGAG CGGATGTACC CCGAGGACGG CGCCCTGAAG GGCGAGATCA 181 AGCAGAGGCT GAAGCTGAAG GACGGCGGCC ACTACGACGC TGAGGTCAAG ACCACCTACA 241 AGGCCAAGAA GCCCGTGCAG CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA 301 CCTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT 361 CCACCGGCGG CATGGACGAG CTGTACAAGT CCGGACTCAG ATCTCGAGCT CAAGCTTCGA 421 ATTCTATGGC CCTCTATTAT GACCACCAGA TAGAAGCCCC GGATGCAGCA GGGTCACCCT 481 CATTTATCAG CTGGCACCCT GTCCATCCAT TCTTGGCAGT TGCTTACATC AGCACAACCT 541 CAACAGGCAG CGTGGATATT TACCTGGAGC AAGGGGAGTG CGTGCCAGAT ACACACGTCG 601 AGAGGCCGTT CCGGGTTGCT TCCCTGTGCT GGCACCCGAC GCGGCTGGTG CTGGCTGTGG 661 GCTGGGAGAC TGGAGAAGTG ACGGTGTTTA ACAAGCAGGA CAAGGAGCAG CACACGATGC 721 CCCTGACACA CACAGCCGAC ATCACCGTGC TCCGTTGGAG CCCCAGTGGA AACTGCCTGC 781 TGTCTGGGGA CAGGCTTGGT TGTCTTGCTC TTGGTGGAGG TTGACCAAGG GGCCGAGTGC 841 AAGGGACGCC CTCTGCTGAA ACACGAGTAT GGAAACACCT CACGCCACTG CATCCTCGCT 901 CCCTCCCTCC TGGCGAGACT GTCAGTGCAA GCAGCTGTGG ACCGTGATGA AAAGCCCTGA 961 CATGTTAACC TGAGAAGAGC AGTTCTGGAG TTGCCTGAAT GGTTCCCGAA GCTGTGTCTT 1021 GCAGTCTGAT GACCGACACT //
Primer: pCAGGS_R1 Region: beta-globinpA,insert 3' Sequence file: RDB15193_A7Fsb.seq
>D04646A4_A7Fs_2_pCAGGS_R1_G08_20_ABI24.ab1 1 NNNNNNNNNN NNNNNCACNN TTGNNNTGNA NNNAAATTTC CTTTATTAGC CAGAAGTCAG 61 ATGCTCAAGG GGCTTCATGA TGTCCCCATA ATTTTTGGCA GAGGGAAAAA GATCCCAGTG 121 GTATTTGTGA GCCAGGGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC 181 CTGAGGAGTC TATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACTC 241 AGGGGTCGTC ATCTGCCTCT TCCACCACCT CCTCGTCCAG CTCCCTGGCG TCCTCCATGC 301 TGTTGTGGCG GACCTGCTCG GGGACGGTGC GTGGCAGTGG GAGACCCAGC CCCCGGTGCA 361 CGGCGTCCAC GGCCTGCGGG CTCACGTAGT AGGACATGTT GGCCAAGGGA AGCCGCCGCC 421 GCATCTCCTC CAGGAATCTG TAGGCCGTCT GGTATTCCTC CTTCCGCACG TAGTGCTCCA 481 CCAGGAAGCC ATAGACGTCC CCGATGCGGA TGGTGCTGTC CAGGTCTGGT TCCTCCAGGA 541 GCAGCTCACA CTGCTTGATG GACTCCTTGG GGTCCTCTGT GTACGTCCTG CGGGCCTGGA 601 TGAACCTCTT CACCAGTGCC ATCCTGCTCT GCAGCTGCGC CAGCCTGGTC TCCTGGTCCA 661 GGGGGCTCTT GGCCTTGGCC TTGGCCAGGC ACTTGTAGGC CTCGGTCAGC GCCCCGTGGG 721 CTTTGTCGTA GTTCTGGTAT TCATCAATCT CCACCTGGGC ACAAGCGTCA TAAAAGCCAG 781 CCAGGAGGTC CAGGGCCCGC CCCTTGGTGT AGAAGCCGAT GANGTTCTTC ATGATCTCCG 841 GCTCCTTCCG CCAGTCCAGG GNNTGCANGT AGTTAGCAGC CATGATGTAN ANTNNCNTCT 901 GCCNNNNNAC GCTCGCGAAN ANNGTGATTT TCNNCGNGNN TNCNGNNTNG AGCAGCGCCN 961 NNNTNGNNNN NCANCTNNNN GNNNGNNNNC NNNNANNTNN NTNNNNNGNN NNNGNNNNNN 1021 NNNN //
Primer: Red-N_R Region: mCherry, CAG pro Sequence file: RDB15193_A7Fsc.seq
>D04646A4_A7Fs_2_Red-N_R_F12_18_ABI24.ab1 1 GATTAATATG CACTTGAGCG CATGACTCCT TGATGATGGC CATGTTATCC TCCTCGCCCT 61 TGCTCACCAT GGTGGCGCTA GCATTTGCCA AAATGATGAG ACAGCACAAT AACCAGCACG 121 TTGCCCAGGA GCTGTAGGAA AAAGAAGAAG GCATGAACAT GGTTAGCAGA GGCTAGAGCC 181 GCCGGTCACA CGCCAGAAGC CGAACCCCGC CCTGCCCCGT CCCCCCCGAA GGCAGCCGTC 241 CCCCCGCGGA CAGCCCCGAG GCTGGAGAGG GAGAAGGGGA CGGCGGCGCG GCGACGCACG 301 AAGGCCCTCC CCGCCCATTT CCTTCCTGCC GGCGCCGCAC CGCTTCGCCC CGCGCCCGCT 361 AGAGGGGGTG CGGCGGCGCC TCCCAGATTT CGGCTCCGCA CAGATTTGGG ACAAAGGAAG 421 TCCCTGCGCC CTCTCGCACG ATTACCATAA AAGGCAATGG CTGCGGCTCG CCGCGCCTCG 481 ACAGCCGCCG GCGCTCCGGG GGCCGCCGCG CCCCTCCCCC GAGCCCTCCC CGGCCCGAGG 541 CGGCCCCGCC CCGCCCGGCA CCCCCACCTG CCGCCACCCC CCGCCCGGCA CGGCGAGCCC 601 CGCGCCACGC CCCGTACGGA GCCCCGCACC CGAAGCCGGG CCGTGCTCAG CAACTCGGGG 661 AGGGGGGTGC AG //