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pcDNA3/hArf5(Q71L)-HA (#RDB15360)

Expression vector of mouse ARF5, constitutively active mutant Q71L.

Clone info. Expression vector of mouse ADP-ribosylation factor5 (ARF5), constitutively active mutant Q71L, tagged with HA epitope at C-ter.
Comment Expression was confirmed by the depositor with immunostaining.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cels).
Gene/insert name human ARF5 cDNA
Depositor|Developer Nakayama, Kazuhisa |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Hanai, A. et al. J. Biochem., 159 (2): 201-208, 2016). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hanai, A. et al. J. Biochem., 159 (2): 201-208, 2016)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15360 pcDNA3/hArf5(Q71L)-HA Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pcDNA3/hArf5(Q71L)-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15360).

Reference section:

Hanai, A., Ohgi, M., Yagi, C., Ueda, T., Shin, H.W., Nakayama, K., Class I Arfs (Arf1 and Arf3) and Arf6 are localized to the Flemming body and play important roles in cytokinesis. J. Biochem. 159 (2): 201-208 (2016). PMID 26330566. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Hanai, A., Class I Arfs (Arf1 and Arf3) and Arf6 are localized to the Flemming body and play important roles in cytokinesis. J. Biochem. 159 (2): 201-208 (2016). PMID 26330566. [link to RRC of NBRP]