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icon below as well as clone information before placing your order.
Expression vector of human tubby like protein 3 (TULP3), tagged with N-terminal EGFP, CMV promoter.
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External Database human TULP3 Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes requires a separate license from the DEPOSITOR, prior to distribution. |
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| Ordering | Order form [Credit Card Payment ] [Bank Transfer Payment]MTA, for use for not-for-profit academic purpose [Word ]MTA, for use for for-profit purpose [Word ]Please visit Information of Request for Distribution.[link] |
[open/close]| 提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hirano, T., Mol. Biol. Cell 28 (3), 429-439, 2017)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。本件リソースを学術研究以外に利用する場合は、事前に寄託者との間で、別途使用許諾にかかる契約の締結が必要である。 |
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| 提供依頼 | 提供依頼書 [Word ]提供同意書 (MTA, 非営利学術目的用)[Word ]提供同意書 (MTA, 営利目的用)[Word ]手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB15194 | pEGFP-C1-TULP3 | DNA solution |
icon below as well as clone information before placing your order.Materials & Methods section:
| The pEGFP-C1-TULP3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15194). |
Reference section:
| Hirano, T., Katoh, Y., Nakayama, K., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
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RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB15194_B3Gkp1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: mVenus-M_F (Pr0574) Region: EGFP 3',insert 5' Sequence file: RDB15194_B3Gka.seq
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>D04646A5_B3Gk_1_mVenus-M_F_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNGGCN TCNAGGTNNN NTTNNAGATC CGCCNCAACA TCGAGGNCGG 61 CAGCGTGCAN NNNNNNNNNN NCTACCAGCA GAACACCCCC ATCGGCGACG GCCCCNNGCT 121 GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA 181 GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA 241 CGAGCTGTAC AAGTCCGGAC TCAGATCTAT GGAGGCTTCG CGCTGCCGGC TCAGTCCCAG 301 CGGCGACAGT GTCTTCCATG AAGAAATGAT GAAGATGCGA CAGGCTAAGC TGGATTATCA 361 GAGGCTACTA CTTGAGAAGA GGCAAAGGAA AAAGCGCCTT GAGCCATTTA TGGTGCAGCC 421 CAATCCAGAA GCCAGGCTAC GTCGGGCAAA GCCAAGGGCC AGTGATGAGC AGACTCCCTT 481 GGTGAACTGT CATACTCCCC ACAGCAATGT CATCTTACAT GGTATTGATG GTCCAGCTGC 541 TGTCCTGAAA CCAGACGAAG TTCATGCTCC ATCAGTAAGC TCCTCTGTTG TGGAAGAAGA 601 TGCTGAAAAC ACCGTGGATA CTGCTTCCAA GCCAGGACTT CAGGAGCGTC TCCAAAAGCA 661 TGATATCTCT GAAAGTGTGA ACTTCGATGA GGAGACTGAT GGAATATCCC AGTCAGCATG 721 TTTAGAAAGA CCCAATTCTG CATCAAGCCA GAATTCAACC GATACAGGCA CTTCCGGTTC 781 TGCTACTGCC GCCCAACCAG CTGATAACCT CCTGNGAGAC ATAGACTACC TGGAGGNNTT 841 TGTGTATAGT CCTGCCCCTC AAGNNGTCAC AGTAANATGT CNGATAATCC GGGNNAAAAN 901 GGGAATGGNT CNGGGNNCNC TNNCCNNNCT ACTATANNNN CTNNGNAAAA AGNNNAAAAT 961 CANNANNANA TTTCTTNNNG NNNNNNNNAN NNNNNNNNAN NNNNNAANNN NNCNNNNNNN 1021 NNN // |
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Primer: SV40polyA_R2 (Pr0623) Region: insert 3' Sequence file: RDB15194_B3Gkb.seq
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>D04646A5_B3Gk_1_SV40polyA_R2_B02_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNTN 61 NTCNGGNNNN NNGNNNNGNG TNNNNNNNNN NNNAANNNNN NNANNNNNNN NNNNNNTGNN 121 NNNNGGCTGA TTATGATCAG TTATCTAGAT CCGGTGGATC CCGGGCCCGC GGTACCGTCG 181 ACTCATTCAC ACGCCAGCTT ACTGTCAAAG CTAGAAAGAC CGATGCCAAA GGCCTGTACT 241 GCACAAAGTG GGTAGTTGTA ATCCAGTGTG AACACGTCAT CTGCCACACG TCCAAACTGC 301 ATGACTATAT AATCAGGGTC ATTTTTGTGG ACTATCTGGA AGTTCTTCAC AGACGCCTGA 361 GTGACCCGGC CACGGAAGTT GAGGACATAG GACTGAGTGT CACTGTTCCA GACGGGGGCC 421 TTGTTGTGCA GCTCAACCAG ATTTTCCATA GTTCTGTTCT GCCACCTTGA GAGCAAACTG 481 TCATGGTTGT TTTGTGGCTG ATAGGGGATC TGCTTATGAT TCAGTGTCAT TCCAGGAATG 541 ATCACAGACA TTTTCCTAGG ACCTTTAAAT CCAAGTACGT TTGTTTCATA GGAGATGGCA 601 GCCAGCTCCT GCCGGGTGTG GGCCGCTCCT ACCAAACCCC GGCCCTTCAT GGGGCAGATG 661 CCACGGTCAT AAACTGTAAA CTTGGTCCCC ATGAGGTTGG ATCTAAGCTT GCCGACATAA 721 CTTTCTCCTT CACGAGNTAA ATCAACTGGA TCAATGGAGA TAAGGTAGTT GGCTGTTTTG 781 CTCTTTTTCC GCTTTCTAGC TGCAAGAANN NNTATCTTCT GATTTTCTTC TTTTTCCNAG 841 TACATATAGT ANGTGGGGAA NAGANCCCGA TNCNTTCCCN NTTTANCCCN GNANTATCCG 901 ACATCTNACT GNGNCACNNN NAGGGGCNNN ANTNTACNNN AAGNNNNNNN NNN // |
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Primer: CMV_Forward (Pr0016) Region: CMV pro,EGFP 5' Sequence file: RDB15194_B3Gkc.seq
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>D04646A5_B3Gk_1_CMV_Forward_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN GCNNNGGGCG GTANGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG 121 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT 181 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT 241 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC 301 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA 361 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA 421 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG 481 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA 541 GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA 601 GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA 661 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAN 721 CACCCAGTCC GCCCTGANCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA 781 GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAN CTGTACAANN NCGGANTCAG 841 ATCTATGGAN GCTTCGCGCN NCCNGNTCAN NNCCAGCGGN NANNNNNNCT TCCNTGNNNA 901 AANGATGAAN ANNCNNNNNN NTNNNNNNNN NTNNNNNNNN NN // |
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Primer: SV40pro_ori_F (Pr0731) Region: NeoR_KanR Sequence file: RDB15194_B3Gkd.seq
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>D04646A5_B3Gk_1_SV40pro_ori_F_D02_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGCC GCCTCNNCCT CTGNNCTNTT CCNGAAGTAG TGAGGANGCT 61 TTTTTGNNNN NNNNNNNTTT TGCAAAGNTC GATCAAGAGA CAGGATGAGG ANNNTTTCGC 121 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC 181 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA 241 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG 301 CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG 361 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG 421 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG 481 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC 541 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA 601 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG CATGCCCGAC 661 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT 721 GGCCGCTTTT CTGGATTCAT CGACTGTGNC CGGCTGGGTG TGGCGGANCG CTATCANGAC 781 ATAGCGTTGG CTACNCGTGA TATTGCTGAA GANCTTGGCG GCGAATGGGC TGACCGCTTC 841 CTCGNGCTTT ACNGNATCGC CGCTCCCGAN TCGCAGCGCA NCGCNNTCTA TCGCCNTCTT 901 GANNANTNNN TCNNANCGGN NNNNNGGGGN NTCNAAATGN NNNANCNNNN NNNNNN // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Hirano, T., Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol. Biol. Cell 28 (3): 429-439 (2017). PMID 27932497. [link to RRC of NBRP] |
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2024.03.26
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