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Koji Nakade's Resource

Koji Nakade's web page in English /  in Japanese.

Learn more about their research, in English  /  in Japanese (by Reseachmap[link])

Catalog #Name of cloneRunning titlePublication
RDB08495pAxCA_MsMyShuttle vector to generate rAd expressing mouse Mesp1 and MyocdUnpublished bioresource
RDB08496AxCA_GATA4Recombinant adenovirus expressing mouse Gata4Unpublished bioresource
RDB08499AxCA_MsMyRecombinant adenovirus expressing mouse Mesp1 and MyocdUnpublished bioresource
RDB08926AxCA_GMTRecombinant adenovirus harbouring mouse Mef2, Tbx5 and GATA4 cDNAUnpublished bioresource
RDB12341pRSV-2AExpression vector of 2A peptide-linked proteins.Kurihara, C., An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytes. Biotechnol. Rep. (Amst.) 12: 26-32 (2016). PMID 28352551. (original). [link to RRC of NBRP]
RDB17460pUC GEB-EcoRVEGFP Reporter/Blasticidin resistance selection marker cassette for constructing knock-in donor vector.Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. (original). [link to RRC of NBRP]
RDB17461pUC TEBl-EcoRVtdTomato Reporter/Bleomycin resistance selection marker cassette for constructing knock-in donor vector.Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. (original). [link to RRC of NBRP]
RDB17462pUC GEBEGFP Reporter/Blasticidin resistance selection marker cassette for constructing knock-in donor vector.Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. (original). [link to RRC of NBRP]
RDB17468pUC tk2Backbone vector for constructing donor vector with single HSV-thymidine kinase.Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. (original). [link to RRC of NBRP]
RDB18669pCRTK2x2-ISL1TEBlDonor vector for knocking-in of tdTomato reporter to ISL1 gene. Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. (original). [link to RRC of NBRP]
RDB18670pCRtk2x2NNBackbone vector for constructing donor vector with double HSV-thymidine kinase. Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. (original). [link to RRC of NBRP]
RDB18671pX330 gIsl1Cas9/guideRNA expression vector for cutting ISL1 gene. Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. (original). [link to RRC of NBRP]
RDB18672pUC-TEZtdTomato Reporter/Bleomycin resistance selection marker cassette for constructing knock-in donor vector.Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. (original). [link to RRC of NBRP]
RDB19551pBS2SK+IA-ORF2Plasmid vector of SARS-CoV2 cDNA encoding ORF2 (Spike protein). Unpublished bioresource
RDB19554pUC-IA-ORF4-10Plasmid vector of SARS-CoV2 cDNA encoding from ORF4 to ORF10.Unpublished bioresource
RDB19569pUCtk2x2Universal donor vector for double-tk CRISPR/Cas9 knock-in system. Kpn I/Asp718I site is available for insertion of homology arm,Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB19570pX459 gEEF1A1Cas9 and guideRNA expression vector for cleavage of EEF1A1 gene. Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB19571pUCtk2x2 EEF1-TEZDonor vector for the tdTomato reporter knock-in at the ORF end of EEF1A1. Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB19572pUCtk2x2-OCT3/4-TEZDonor vector for the tdTomato reporter knock-in at the ORF end of OCT3/4. Selection of knocked-in clone using the double-tk system is available.Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB19586pUC-OCT3/4-TEZDonor vector for the tdTomato reporter knock-in at the ORF end of OCT3/4.Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]

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2024.04.04

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