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Koji Nakade's web page in English / in Japanese
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| Catalog # | Name of clone | Running title | Publication |
| RDB19586 | pUC-OCT3/4-TEZ | Donor vector for the tdTomato reporter knock-in at the ORF end of OCT3/4. | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
| RDB19572 | pUCtk2x2-OCT3/4-TEZ | Donor vector for the tdTomato reporter knock-in at the ORF end of OCT3/4. Selection of knocked-in clone using the double-tk system is available. | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
| RDB19571 | pUCtk2x2 EEF1-TEZ | Donor vector for the tdTomato reporter knock-in at the ORF end of EEF1A1. | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
| RDB19570 | pX459 gEEF1A1 | Cas9 and guideRNA expression vector for cleavage of EEF1A1 gene. | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
| RDB19569 | pUCtk2x2 | Universal donor vector for double-tk CRISPR/Cas9 knock-in system. Kpn I/Asp718I site is available for insertion of homology arm, | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
| RDB19554 | pUC-IA-ORF4-10 | Plasmid vector of SARS-CoV2 cDNA encoding from ORF4 to ORF10. | Unpublished bioresource |
| RDB19551 | pBS2SK+IA-ORF2 | Plasmid vector of SARS-CoV2 cDNA encoding ORF2 (Spike protein). | Unpublished bioresource |
| RDB18672 | pUC-TEZ | tdTomato Reporter/Bleomycin resistance selection marker cassette for constructing knock-in donor vector. | Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. (original). [link to RRC of NBRP] |
| RDB18671 | pX330 gIsl1 | Cas9/guideRNA expression vector for cutting ISL1 gene. | Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. (original). [link to RRC of NBRP] |
| RDB18670 | pCRtk2x2NN | Backbone vector for constructing donor vector with double HSV-thymidine kinase. | Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. (original). [link to RRC of NBRP] |
| RDB18669 | pCRTK2x2-ISL1TEBl | Donor vector for knocking-in of tdTomato reporter to ISL1 gene. | Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. (original). [link to RRC of NBRP] |
| RDB17468 | pUC tk2 | Backbone vector for constructing donor vector with single HSV-thymidine kinase. | Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. (original). [link to RRC of NBRP] |
| RDB17462 | pUC GEB | EGFP Reporter/Blasticidin resistance selection marker cassette for constructing knock-in donor vector. | Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. (original). [link to RRC of NBRP] |
| RDB17461 | pUC TEBl-EcoRV | tdTomato Reporter/Bleomycin resistance selection marker cassette for constructing knock-in donor vector. | Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. (original). [link to RRC of NBRP] |
| RDB17460 | pUC GEB-EcoRV | EGFP Reporter/Blasticidin resistance selection marker cassette for constructing knock-in donor vector. | Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. (original). [link to RRC of NBRP] |
| RDB12341 | pRSV-2A | Expression vector of 2A peptide-linked proteins. | Kurihara, C., An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytes. Biotechnol. Rep. (Amst.) 12: 26-32 (2016). PMID 28352551. (original). [link to RRC of NBRP] |
| RDB08926 | AxCA_GMT | Recombinant adenovirus harbouring mouse Mef2, Tbx5 and GATA4 cDNA | Unpublished bioresource |
| RDB08499 | AxCA_MsMy | Recombinant adenovirus expressing mouse Mesp1 and Myocd | Unpublished bioresource |
| RDB08496 | AxCA_GATA4 | Recombinant adenovirus expressing mouse Gata4 | Unpublished bioresource |
| RDB08495 | pAxCA_MsMy | Shuttle vector to generate rAd expressing mouse Mesp1 and Myocd | Unpublished bioresource |
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2025.03.28
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