Cas9/guideRNA expression vector for cutting ISL1 gene.
Clone info. | Cas9/guideRNA expression vector for cutting ISL1 gene. For more information, See Tsukamoto, S. et al., Stem Cell Res., 53: 102363, 2021. |
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Vector backbone | pX330 (plasmid) |
Size of vector backbone | 4.3 kb |
Growth conditions | LB+Amp, 37oC |
Gene/insert name | human ISL1 synthetic gRNA. |
Depositor|Developer | Nakade, Koji | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021) |
Remarks | The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations. |
必要書類 | 提供依頼書 ![]() ![]() 専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word] ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)。 |
備考 | 本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB18671 | pX330 gIsl1 | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The pX330 gIsl1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18671). |
Reference section:
Tsukamoto, S., Nakade, K., Wakabayashi, T., Nakashima, K., Takami, M., Hemmi, Y., Kuramochi, Y., Shimizu, T., Arai, Y., Matsuo-Takasaki, M., Noguchi, M., Nakamura, Y., Miwa, Y., Hayashi, Y., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP] |
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reference | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP] |
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