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pUCtk2x2 EEF1-TEZ (#RDB19571)

Donor vector for the tdTomato reporter knock-in at the ORF end of EEF1A1.

Alternative name pUCtk2x2 EEF1-TEBl
Clone info. Donor vector for the tdTomato reporter knock-in at the ORF end of EEF1A1. Selection of knocked-in clone using the double-tk system is available.
Vector backbone pUCtk2x2 (RDB19569) (plasmid)
Gene/insert name Herpes simplex virus (HSV) thymidin kinase (TK) gene; human EEF1A1 genomic DNA; Dscosoma sp. red fluorescent protein (tdTomato); human EEF1A1 promoter; Streptoalloteichus hindustanus bleomycin resistant gene.
Depositor|Developer DNA Bank, | Nakade, Koji |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.　(Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))。

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RDB19571 pUCtk2x2 EEF1-TEZ Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pUCtk2x2 EEF1-TEZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19571).

Reference section:

Nakade, K., Tsukamoto, S., Nakashima, K., An, Y., Sato, I., Li, J., Shimoda, Y., Hemmi, Y., Miwa, Y., Hayashi, Y., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]