Resource data sheet
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pCRtk2x2NN (#RDB18670)

Backbone vector for constructing donor vector with double HSV-thymidine kinase.

Clone info. Backbone vector for constructing donor vector with double HSV-thymidine kinase. For more information, See Tsukamoto, S. et al., Stem Cell Res., 53: 102363, 2021.
Comment 2xHSV-tk was from RDB01692 pCM-TK
Vector backbone pCR Blunt II (plasmid)
Size of vector backbone 3.5 kb
Growth conditions LB+Kanamycin, 37oC
Depositor|Developer Nakade, Koji |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18670 pCRtk2x2NN Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pCRtk2x2NN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18670).

Reference section:

Tsukamoto, S., Nakade, K., Wakabayashi, T., Nakashima, K., Takami, M., Hemmi, Y., Kuramochi, Y., Shimizu, T., Arai, Y., Matsuo-Takasaki, M., Noguchi, M., Nakamura, Y., Miwa, Y., Hayashi, Y., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP]
reference Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]
protocol Hayashi, Y., Generation of Reporter Human Pluripotent Stem Cells Using CRISPR/Cas9 Editing. In: Ding, B., Tang, Y. (eds) Human Induced Pluripotent Stem Cells. Neuromethods, vol 210. Humana, New York, NY (2024). [link to Journal]
user_report Wakimoto, A., Intraplacental injection of human iPSC-derived PDX1+ pancreatic progenitors prolongs Pdx1-deficient mice survival. bioRxiv doi: 10.1101/2024.05.09.593461 (2024). [link to Journal]