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pCRTK2x2-ISL1TEBl (#RDB18669)

Donor vector for knocking-in of tdTomato reporter to ISL1 gene.

Clone info. Donor vector for knocking-in of tdTomato reporter to ISL1 gene. For more information, See Tsukamoto, S. et al., Stem Cell Res., 53: 102363, 2021.
Comment 2xHSVtk was from pCM-TK (RDB01692).
Vector backbone pCR Blunt II (plasmid)
Size of vector backbone 3.5 kb
Growth conditions LB+Kanamycin, 37oC
Depositor|Developer Nakade, Koji |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)
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RDB18669 pCRTK2x2-ISL1TEBl Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pCRTK2x2-ISL1TEBl was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18669).

Reference section:

Tsukamoto, S., Nakade, K., Wakabayashi, T., Nakashima, K., Takami, M., Hemmi, Y., Kuramochi, Y., Shimizu, T., Arai, Y., Matsuo-Takasaki, M., Noguchi, M., Nakamura, Y., Miwa, Y., Hayashi, Y., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [PubMed] [Article] [RRC of NBRP]
reference Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [PubMed] [Article] [RRC of NBRP]