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pCRTK2x2-ISL1TEBl (#RDB18669)

Donor vector for knocking-in of tdTomato reporter to ISL1 gene.

Clone info. Donor vector for knocking-in of tdTomato reporter to ISL1 gene. For more information, See Tsukamoto, S. et al., Stem Cell Res., 53: 102363, 2021.
Comment 2xHSVtk was from RDB01692 pCM-TK
Vector backbone pCR Blunt II (plasmid)
Size of vector backbone 3.5 kb
Growth conditions LB+Kanamycin, 37oC
Depositor|Developer Nakade, Koji |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18669 pCRTK2x2-ISL1TEBl Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pCRTK2x2-ISL1TEBl was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18669).

Reference section:

Tsukamoto, S., Nakade, K., Wakabayashi, T., Nakashima, K., Takami, M., Hemmi, Y., Kuramochi, Y., Shimizu, T., Arai, Y., Matsuo-Takasaki, M., Noguchi, M., Nakamura, Y., Miwa, Y., Hayashi, Y., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP]
reference Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]