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pUC TEBl-EcoRV (#RDB17461)

tdTomato Reporter/Bleomycin resistance selection marker cassette for constructing knock-in donor vector.

Clone info. tdTomato Reporter/Bleomycin resistance selection marker cassette for constructing knock-in donor vector. The cassette will be liberated by EcoRV digestion.
Vector backbone pUC19 (plasmid)
Size of vector backbone 2.7 kb
Gene/insert name Discosoma sp. RFP (tdTomato); Streptoalloteichus hindustanus bleomycin resistance gene.
Depositor|Developer Nakade, Koji |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17461 pUC TEBl-EcoRV Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pUC TEBl-EcoRV was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17461).

Reference section:

Tsukamoto, S., Nakade, K., Wakabayashi, T., Nakashima, K., Takami, M., Hemmi, Y., Kuramochi, Y., Shimizu, T., Arai, Y., Matsuo-Takasaki, M., Noguchi, M., Nakamura, Y., Miwa, Y., Hayashi, Y., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP]