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pUC-IA-ORF4-10 (#RDB19554)

Plasmid vector of SARS-CoV2 cDNA encoding from ORF4 to ORF10.

Clone info. Plasmid vector of SARS-CoV2 cDNA encoding from ORF4 to ORF10. The cDNA was amplified from the genomic RNA of hCoV-19/Japan/NGS-IA-1/2020 (GISAID accession no. EPI-ISL-481251) by RT-PCR and cloned into the KpnI site of the pUC19 vector.
Comment KpnI. It can be liberated by SphI and SacI double digestion.
Vector backbone pUC19 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name SARS-CoV-2 ORF4,5,6,7,8,9 and 10 cDNA
Depositor|Developer DNA Bank, | Nakade, Koji |
 

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Terms and conditions for distribution The RECIPIENT agrees to expressly describe the acknowledgement of the NBRP Human Pathogenic Viruses as the source of the BIOLOGICAL RESOURCE in any publication. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to obtain approval from the Institute of Tropical Medicine, Nagasaki University.
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RDB19554 pUC-IA-ORF4-10 Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pUC-IA-ORF4-10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19554).

Reference section:

Further references such as user reports and related articles (go to bottom)


QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles