Resource data sheet
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pRSV-2A

Expression vector of 2A peptide-linked proteins.

Catalog number RDB12341
Resource name pRSV-2A
Alternative name pRC-2A
Clone info. Expression vector of 2A peptide-linked proteins.
Vector backbone pRc/RSV (Plasmid)
Selectable markers Amp^r
Depositor DNA Bank, | Nakade, Koji |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR Acknowledgement to Developer in publication.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12341 pRSV-2A DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Kurihara, C., An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytes. Biotechnol. Rep. (Amst.) 12: 26-32 (2016). PMID 28352551.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12341_A3Lp.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: RDB12341_A3Lpa.seq
>RDB12341_A3Lp_RSV_Pro_F_D09_12.ab1
    1 TAGTTTGGAC ATTCACCACA TTGGGTGTGC ACCTCCAAGC TTGGTACCGA GCTCGGATCC
   61 ACTAGTGAAG GTCGTGGTTC TTTATTAACT TGTGGTGATG TTGAAGAAAA TCCTGGTCCT
  121 GCTAGCTGAG GTACCGCGGC CGCTCGAGCA TGCATCTAGA GCTCGCTGAT CAGCCTCGAC
  181 TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT
  241 GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT
  301 GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG
  361 GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG
  421 AACCAGCTGG GGCTCGAGGG GGGATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC
  481 GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC
  541 TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA
  601 TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT
661 TGATTAGGGT GATGG
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Please visit Sequencing and PCR primers for primer information.


References

References

original Kurihara, C., An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytes. Biotechnol. Rep. (Amst.) 12: 26-32 (2016). PMID 28352551.

2018.12.10

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