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pRSV-2A

Expression vector of 2A peptide-linked proteins.

Catalog number RDB12341
Resource name pRSV-2A
Alternative name pRC-2A
Clone info. Expression vector of 2A peptide-linked proteins.
Vector backbone pRc/RSV (Plasmid)
Selectable markers Amp^r
Depositor|Developer DNA Bank, | Nakade, Koji |
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            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution Acknowledgement to Developer in publication.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
            提供案内 (日本国内) [open/close]

            提供条件 研究結果の発表時、謝辞に樹立者名を入れること。
            利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB12341 pRSV-2A DNA solution Please contact us.

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pRSV-2A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12341).

            Reference section:

            Kurihara, C., Nakade, K., Pan, J., Huang, J., Wasylyk, B., Obata, Y., An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytes. Biotechnol. Rep. (Amst.) 12: 26-32 (2016). PMID 28352551. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12341_A3Lp.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer:
            Sequence file: RDB12341_A3Lpa.seq check
            >RDB12341_A3Lp_RSV_Pro_F_D09_12.ab1
                1 TAGTTTGGAC ATTCACCACA TTGGGTGTGC ACCTCCAAGC TTGGTACCGA GCTCGGATCC
               61 ACTAGTGAAG GTCGTGGTTC TTTATTAACT TGTGGTGATG TTGAAGAAAA TCCTGGTCCT
              121 GCTAGCTGAG GTACCGCGGC CGCTCGAGCA TGCATCTAGA GCTCGCTGAT CAGCCTCGAC
              181 TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT
              241 GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT
              301 GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG
              361 GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG
              421 AACCAGCTGG GGCTCGAGGG GGGATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC
              481 GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC
              541 TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA
              601 TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT
            661 TGATTAGGGT GATGG
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Kurihara, C., An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytes. Biotechnol. Rep. (Amst.) 12: 26-32 (2016). PMID 28352551. [link to RRC of NBRP]

            2023.08.18

            GNP_filter3_RDBDEP_html_230702.pl