Please review the QC test results indicated by icon below as well as clone information before placing your order.

pUC-TEZ

tdTomato Reporter/Bleomycin resistance selection marker cassette for constructing knock-in donor vector.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
Remarks	This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

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提供条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
備考	Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18672_B2Gjp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F2 (Pr0739) Region: lac pro,lac O,T2A,tdTomato 5' Sequence file: RDB18672_B2Gja.seq
>D07748A1_B2Gj_1_pUC-CAPbs_up_F2_A04_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NGCTTTNNNC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA    61 ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACTAGTGAAG GTCGTGGTTC   121 TTTATTAACT TGTGGTGATG TTGAAGAAAA TCCTGGTCCT GCTAGCATGG TGAGCAAGGG   181 CGAGGAGGTC ATCAAAGAGT TCATGCGCTT CAAGGTGCGC ATGGAGGGCT CCATGAACGG   241 CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC   301 CAAGCTGAAG GTGACCAANG GCGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCCCA   361 GTTCATGTAC GGCTCCAAGG CGTACGTGAA GCACCCCGCC GACATCCCCG ATTACNNNNN   421 NNNGTCNTTC CCNGAGGGCT TCAAGTGGGA GCGCGTGATG AACTTNNNNG ACGGCGGTCT   481 GGTGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCNCN NNNATCTACN AGGTGAANAT   541 GCNCGGCACN NANTTCCCCC CCNACGGCCC CGTANTGNNN AANAANACCA TGGNNNGGNA   601 GGCCTCCACC GANCNNNNGN ACCCCCGCGA CNGCGTGCTG AAAGGGCGAG ATNNANCNNN   661 CCCTGAAANC TGAAGGANNG NGGCNNNTAN NNNNTGGANN NNNNNANNCN TCTNNNNNNC   721 CAANAANNNC NGTGCANNNG NNCGGCTACT ACNACNTGNA CNCNAAGCTG GACNTNANCT 781 CCCNNNNNNA NNNNNNCACC ATCNTGNNNN NNNNNCNNNN NNNNNN //
Primer: pUC-M13_-100 (Pr0310) Region: BleoR,EF-1alpha pro Sequence file: RDB18672_B2Gjb.seq
>D07748A1_B2Gj_1_pUC-M13_-100_B04_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NGGNNNNNNN NGGGTTTTCC NNGTCACGAC GTTGTAAAAC    61 GACGGCCAGT GAATTTCTAG ATTAGTCCTG CTCCTCGGCC ACGAAGTGCA CGCAGTTGCC   121 GGCCGGGTCG CGCAGGGCGA ACTCCCGCCC CCACGGCTGC TCGCCGATCT CGGTCATGGC   181 CGGCCCGGAG GCGTCCCGGA AGTTCGTGGA CACGACCTCC GACCACTCGG CGTACAGCTC   241 GTCCAGGCCG CGCACCCACA CCCAGGCCAG GGTGTTGTCC GGCACCACCT GGTCCTGGAC   301 CGCGCTGATG AACAGGGTCA CGTCGTCCCG GACCACACCG GCGAAGTCGT CCTCCACGAA   361 GTCCCGGGAG AACCCGAGCC GGTCGGTCCA GAACTCGACC GCTCCGGCGA CGTCGCGCGC   421 GGTGAGCACC GGAACGGCAC TGGTCAACTT GGCCATACTA GAGTGTTCAC GACACCTGAA   481 ATGGAAGAAA AAAACTTTGA ACCACTGTCT GAGGCTTGAG AATGAACCAA GATCCAAACT   541 CAAAAAGGGC AAATTCCAAG GAGAATTACA TCAAGTGCCA AGCTGGCCTA ACTTCAGTCT   601 CCACCCACTC AGTGTGGGGA AACTCCATCG CATAAAACCC CTCCCCCCAA CCTAAAGACG   661 ACGTACTCCA AAAGCTCGAG AACTAATCGA GGTGCCTGGA CGGCGCCCGG TACTCCGTGG   721 AGTCACATGA AGCGACGGCT GANGANGGAA AGGCCCTTTT CCTTTGTGTG GNTGANTCAC   781 CCGCCCGCTC TCCCGAGCGC CGCGTCCTCC ATTTTGAGCT CCCTGCANCN NGGCCGGGAA   841 NCGGCCATCT TTCCGCTCAC GCAACTGGTG NCNANCGGGC CAGCCNNNGC CGCCCAGGGC   901 GGGGCGATAC ACGGCGGCGC GNNGNCNNNN CACCANANNN NNNCNGNNAN CTNNAGANNA 961 NCCCCCNNCC NANNNNNNNN NN //
Primer: tdTomato-2N_R (Pr1238) Region: tdTomato 5',T2A Sequence file: RDB18672_B2Gjc.seq
>D07748A1_B2Gj_1_tdTomato-2N_R_C05_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN GGNGCCGGNN CTGCCGCTGC CGGTGCTGCC GGTGCCNTGC    61 CCCAGGAACA GGTGNTGGCG GCCCTCGGAG CGCTCGTACT GTTCCACGAT GGTGTAGTCC   121 TCGTTGTGGG AGGTGATGTC CAGCTTGGTG TCCACGTAGT AGTAGCCGGG CAGTTGCACG   181 GGCTTCTTGG CCATGTAGAT GGTCTTGAAC TCCACCAGGT AGTGGCCGCC GTCCTTCAGC   241 TTCAGGGCCT GGTGGATCTC GCCCTTCAGC ACGCCGTCGC GGGGGTACAG GCGCTCGGTG   301 GAGGCCTCCC AGCCCATGGT CTTCTTCTGC ATTACGGGGC CGTCGGGGGG GAAGTTGGTG   361 CCGCGCATCT TCACCTTGTA GATCAGCGTG CCGTCCTGCA GGGAGGAGTC CTGGGTCACG   421 GTCACCAGAC CGCCGTCCTC GAAGTTCATC ACGCGCTCCC ACTTGAAGCC CTCGGGGAAG   481 GACAGCTTCT TGTAATCGGG GATGTCGGCG GGGTGCTTCA CGTACGCCTT GGAGCCGTAC   541 ATGAACTGGG GGGACAGGAT GTCCCAGGCG AAGGGCAGGG GGCCGCCCTT GGTCACCTTC   601 AGCTTGGCGG TCTGGGTGCC CTCGTAGGGG CGGCCCTCGC CCTCGCCCTC GATCTCGAAC   661 TCGTGGCCGT TCATGGAGCC CTCCATGCGC ACCTTGAAGC GCATGAACTC TTTGATGACC   721 TCCTCGCCCT TGCTCACCAT GCTAGCAGGA CCAGGATTTT CTTCAACATC ACCACAAGTT   781 AATAAAGAAC CACGACCTTC ACTAGTCATA GCTGTTTCCT GTGTGAAANT GTTATCCGCT   841 CACAATTCCA CACAACATAC GAGCCGGAAG CATNAAGTGT AAAGNCTNNN GTGNCTNATG   901 AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCNNTGCCC GCTTNNCAGT NGGNAAANCT   961 GTCGNGCNNN CTGCNTTNNT GAATCGGNNA ACGCNCGGGG AGANGNGGNT NGNNNATTGG 1021 GNNNTCTTNN NNTTNCNNGC NNANTGNNNN NNNNNN //
Primer: tdTomato-2C_R (Pr1239) Region: tdTomato mid Sequence file: RDB18672_B2Gjd.seq
>D07748A1_B2Gj_1_tdTomato-2C_R_D05_04_ABI08.ab1     1 NNNNNNNNNN NNNNGNNCNN NNGNNNTGTT CNNCGATGGT GTAGTCCTCG TTGTGGGAGG    61 TGATGTCCAG NTTGNTGTCC ACGTAGTAGT AGCCGGGCAG TTGCACGGGC TTCTTGGCCA   121 TGTAGATGGT CTTGAACTCC ACCAGGTAGT GGCCGCCGTC CTTCAGCTTC AGGGCCTGGT   181 GGATCTCGCC CTTCAGCACG CCGTCGCGGG GGTACAGGCG CTCGGTGGAG GCCTCCCAGC   241 CCATGGTCTT CTTCTGCATT ACGGGGCCGT CGGGGGGGAA GTTGGTGCCG CGCATCTTCA   301 CCTTGTAGAT CAGCGTGCCG TCCTGCAGGG AGGAGTCCTG GGTCACGGTC ACCAGACCGC   361 CGTCCTCGAA GTTCATCACG CGCTCCCACT TGAAGCCCTC GGGGAAGGAC AGCTTCTTGT   421 AATCGGGGAT GTCGGCGGGG TGCTTCACGT ACGCCTTGGA GCCGTACATG AACTGGGGGG   481 ACAGGATGTC CCAGGCGAAG GGCAGGGGGC CGCCCTTGGT CACCTTCAGC TTGGCGGTCT   541 GGGTGCCCTC GTAGGGGCGG CCCTCGCCCT CGCCCTCGAT CTCGAACTCG TGGCCGTTCA   601 TGGAGCCCTC CATGCGCACC TTGAAGCGCA TGAACTCTTT GATGACGGCC ATGTTGTTGT   661 CCTCGGAGGA GGCGGTGCCG GAGCTGCCGC TGCCGGTGCT GCCGGTGCCA TGCCCCAGGA   721 ACAGGTGGTG GCGGCCCTCG GAGCGCTCGT ACTGTTCCAC GATGGTGTAG TCCTCGTTGT   781 GGGAGGTGAN GTCCAGCTTG GTGTCCACGT AGTAGTAGCC GGGCAGTTGC NCGGGCTTCT   841 TGNCCATGTA GATGGNCTTG AACTCCACCA GGTAGTGGCC GCCGTCCTTC AGCTTCNGGG   901 CCTGGTGNNN CTCGNCCTTC ANCANGCCGT CNCGGGGGTN CNGGNGCTCG GNGNANGNCT   961 NCCANCCNNT GGNCTTCNNC TGNANTACGG GGCCGTCNGG GGGGNNGTTG GTGNCNNNNN 1021 TNNNCNNCNT NNNANATNAN CNNGNCNNNN NNNNNN //
Primer: EF1a-N_R Sequence file: RDB18672_B2Gje.seq
>D07748A1_B2Gj_1_EF1a-N_R_C04_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNGN CGNNGTGCGC TCTGCCNNCT GACGGGCACC GGNNCCTCAC    61 GACTAGCCCA TAGAGCCCAC CGCATCCCCA GCATGCCTGC TATTGTCTTC CCAATCCTCC   121 CCCTTGCTGT CCTGCCCCAC CCCACCCCCC AGAATAGAAT GACACCTACT CAGACAATGC   181 GATGCAATTT CCTCATTTTA TTAGGAAAGG ACAGTGGGAG TGGCACCTTC CAGGGTCAAG   241 GAAGGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC AGGCTAGCCT   301 ACTTGTACAG CTCGTCCATG CCGTACAGGA ACAGGTGGTG GCGGCCCTCG GAGCGCTCGT   361 ACTGTTCCAC GATGGTGTAG TCCTCGTTGT GGGAGGTGAT GTCCAGCTTG GTGTCCACGT   421 AGTAGTAGCC GGGCAGTTGC ACGGGCTTCT TGGCCATGTA GATGGTCTTG AACTCCACCA   481 GGTAGTGGCC GCCGTCCTTC AGCTTCAGGG CCTGGTGGAT CTCGCCCTTC AGCACGCCGT   541 CGCGGGGGTA CAGGCGCTCG GTGGAGGCCT CCCAGCCCAT GGTCTTCTTC TGCATTACGG   601 GGCCGTCGGG GGGGAAGTTG GTGCCGCGCA TCTTCACCTT GTAGATCAGC GTGCCGTCCT   661 GCAGGGAGGA GTCCTGGGTC ACGGTCACCA GACCGCCGTC CTCGAAGTTC ATCACGCGCT   721 CCCACTTGAA GCCCTCGGGG AAGGACAGCT TCTTGTAATC GGGGATGTCG GCGGGGTGCT   781 TCACGTACGC CTTGGAGCCG TACATGANCT GGGGGGGACA NGANGTCCCN ANGCGAANGG 841 CANGGGGNCN NCCCTTGGTC NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl