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pUC-TEZ (#RDB18672)

tdTomato Reporter/Bleomycin resistance selection marker cassette for constructing knock-in donor vector.


Drawn by SnapGene® software
Sequence (full) RDB18672hts02.seq
Publication Tsukamoto, S., Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP]
Test sheet RDB18672_B3Fep1-2.pdf 
Assembled from experimentally sequenced data.
 
Alternative name pUC19-TEBl
Clone info. tdTomato Reporter/Bleomycin resistance selection marker cassette for constructing knock-in donor vector. The cassette will be liberated by SpeI/XbaI digestion. For more information, See Tsukamoto, S. et al., Stem Cell Res., 53: 102363, 2021.
Vector backbone pUC19 (plasmid)
Size of vector backbone 2.7 kb
Growth conditions LB+Amp, 37oC
Gene/insert name Discosoma sp. RFP (tdTomato) cDNA; Streptoalloteichus hindustanus bleomycin resistance gene.
Depositor|Developer Nakade, Koji |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18672 pUC-TEZ DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

How to cite this biological resource

Materials & Methods section:

The pUC-TEZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18672).

Reference section:

Tsukamoto, S., Nakade, K., Wakabayashi, T., Nakashima, K., Takami, M., Hemmi, Y., Kuramochi, Y., Shimizu, T., Arai, Y., Matsuo-Takasaki, M., Noguchi, M., Nakamura, Y., Miwa, Y., Hayashi, Y., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP]
reference Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]
protocol Hayashi, Y., Generation of Reporter Human Pluripotent Stem Cells Using CRISPR/Cas9 Editing. In: Ding, B., Tang, Y. (eds) Human Induced Pluripotent Stem Cells. Neuromethods, vol 210. Humana, New York, NY (2024). [link to Journal]
user_report Wakimoto, A., Intraplacental injection of human iPSC-derived PDX1+ pancreatic progenitors prolongs Pdx1-deficient mice survival. bioRxiv doi: 10.1101/2024.05.09.593461 (2024). [link to Journal]