tdTomato Reporter/Bleomycin resistance selection marker cassette for constructing knock-in donor vector.
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Alternative name | pUC19-TEBl |
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Clone info. | tdTomato Reporter/Bleomycin resistance selection marker cassette for constructing knock-in donor vector. The cassette will be liberated by SpeI/XbaI digestion. For more information, See Tsukamoto, S. et al., Stem Cell Res., 53: 102363, 2021. |
Vector backbone | pUC19 (plasmid) |
Size of vector backbone | 2.7 kb |
Growth conditions | LB+Amp, 37oC |
Gene/insert name | Discosoma sp. RFP (tdTomato) cDNA; Streptoalloteichus hindustanus bleomycin resistance gene. |
Depositor|Developer | Nakade, Koji | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021) |
必要書類 | 提供依頼書 ![]() ![]() 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18672 | pUC-TEZ | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein |
Materials & Methods section:
The pUC-TEZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18672). |
Reference section:
Tsukamoto, S., Nakade, K., Wakabayashi, T., Nakashima, K., Takami, M., Hemmi, Y., Kuramochi, Y., Shimizu, T., Arai, Y., Matsuo-Takasaki, M., Noguchi, M., Nakamura, Y., Miwa, Y., Hayashi, Y., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP] |
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reference | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP] |
protocol | Hayashi, Y., Generation of Reporter Human Pluripotent Stem Cells Using CRISPR/Cas9 Editing. In: Ding, B., Tang, Y. (eds) Human Induced Pluripotent Stem Cells. Neuromethods, vol 210. Humana, New York, NY (2024). [link to Journal] |
user_report | Wakimoto, A., Intraplacental injection of human iPSC-derived PDX1+ pancreatic progenitors prolongs Pdx1-deficient mice survival. bioRxiv doi: 10.1101/2024.05.09.593461 (2024). [link to Journal] |
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