RDB18672 - pUC-TEZ

pUC-TEZ (#RDB18672)

tdTomato Reporter/Bleomycin resistance selection marker cassette for constructing knock-in donor vector.

Drawn by SnapGene® software

Sequence information (Assembled from experimentally sequenced data)	GenBank Flat File Format open
	SnapGene file download
Publication	Tsukamoto, S., Stem Cell Res. 53: 102363 (2021). PMID 34087992. [PubMed] [Article] [RRC of NBRP]
Test sheet	Data Sheet open

Alternative name	pUC19-TEBl
Clone info.	tdTomato Reporter/Bleomycin resistance selection marker cassette for constructing knock-in donor vector. The cassette will be liberated by SpeI/XbaI digestion. For more information, See Tsukamoto, S. et al., Stem Cell Res., 53: 102363, 2021.
Vector backbone	pUC19 (plasmid)
Size of vector backbone	2.7 kb
Growth conditions	LB+Amp, 37℃
Gene/insert name	Discosoma sp. RFP (tdTomato) cDNA; Streptoalloteichus hindustanus bleomycin resistance gene.
Depositor\|Developer	Nakade, Koji \|

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)
Additional terms and conditions:
Further transfer, provision of services or resale of the Zeocin resistance gene, and the Deposited Materials is prohibited without InvivoGen's prior written consent. In addition, InvivoGen’s Limited Use Label License applies to your use of this product.

Remarks
Zeocin® is a trademark of InvivoGen registered with the United States Patent and Trademark Office and foreign equivalents. See InvivoGen Limited Use Label License.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] In order to receive this biological resource, please use the Exclusive MTA [Link]. Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021) Additional terms and conditions: Further transfer, provision of services or resale of the Zeocin resistance gene, and the Deposited Materials is prohibited without InvivoGen's prior written consent. In addition, InvivoGen’s Limited Use Label License applies to your use of this product.
Remarks	Zeocin® is a trademark of InvivoGen registered with the United States Patent and Trademark Office and foreign equivalents. See InvivoGen Limited Use Label License.

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MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)。付加的使用条件： InvivoGen社の事前の書面による同意がない限り、ゼオシン耐性遺伝子および寄託マテリアルの第三者への移転、サービスの提供、または再販売は禁止されています。また、ご利用に際してInvivoGen社のLimited Use Label Licenseが適用されます。
備考	ZeocinはInvivoGen社の製品です。Zeocin®は、米国特許商標庁および米国外の同等機関に登録されたInvivoGen社の商標です。InvivoGen Limited Use Label Licenseをご確認ください。

Catalog #	Resource name	Shipping form	Fee
RDB18672	pUC-TEZ	DNA solution

How to cite this biological resource

Materials & Methods section:

The pUC-TEZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18672).

Reference section:

Tsukamoto, S., Nakade, K., Wakabayashi, T., Nakashima, K., Takami, M., Hemmi, Y., Kuramochi, Y., Shimizu, T., Arai, Y., Matsuo-Takasaki, M., Noguchi, M., Nakamura, Y., Miwa, Y., Hayashi, Y., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [PubMed] [Article] [RRC of NBRP]
reference	Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [PubMed] [Article] [RRC of NBRP]
protocol	Hayashi, Y., Generation of Reporter Human Pluripotent Stem Cells Using CRISPR/Cas9 Editing. In: Ding, B., Tang, Y. (eds) Human Induced Pluripotent Stem Cells. Neuromethods, vol 210. Humana, New York, NY (2024). [Article]
user_report	Wakimoto, A., Intraplacental injection of human iPSC-derived PDX1+ pancreatic progenitors prolongs Pdx1-deficient mice survival. bioRxiv 2024.05.09.593461 (2024). [Article]

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