Shuttle vector to generate rAd expressing mouse Mesp1 and Myocd
Alternative name | pAxCA_Mesp1_T2A_Myocd U3334W.c3 |
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Clone info. | Shuttle vector to generate rAd expressing mouse Mesp1 and Myocd. Mesp1 and Myocd were fused with T2A peptide ,which contains self clearvage amino acid sequence. |
Comment | Nucleotide substitution C333A (Arg94Ser) and T510C (Cys153Arg) to NM_008588.2 of Mesp1. Nucleotide substitution G3194A (Met silent) to NM._145136.4 of Myocd. |
Vector backbone | pAxCAwtit2 (Cosmid, use packaging extracts for transforming E. coli host) |
Gene/insert name | Mouse Mesp1 cDNA Mouse Myocd |
Depositor|Developer | DNA Bank, | Nakade, Koji | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | This material is provided with written consent of Developer(nakade@rtc.riken.jp). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested. |
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Remarks | Remember that you will be working with samples containing infectious virus. |
MTAに書く使用条件 | 事前に樹立者(nakade@rtc.riken.jp)に連絡し、提供承諾を得る。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。 MTAに書く付加的使用条件: CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。 |
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備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB08495 | pAxCA_MsMy | Under QC test. Please contact us. | DNA solution | JPY 32,340 plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pAxCA_MsMy was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08495). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
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