Resource data sheet
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pAxCA_MsMy (#RDB08495)

Shuttle vector to generate rAd expressing mouse Mesp1 and Myocd

Alternative name pAxCA_Mesp1_T2A_Myocd U3334W.c3
Clone info. Shuttle vector to generate rAd expressing mouse Mesp1 and Myocd. Mesp1 and Myocd were fused with T2A peptide ,which contains self clearvage amino acid sequence.
Comment Nucleotide substitution C333A (Arg94Ser) and T510C (Cys153Arg) to NM_008588.2 of Mesp1. Nucleotide substitution G3194A (Met silent) to NM._145136.4 of Myocd.
Vector backbone pAxCAwtit2 (Cosmid, use packaging extracts for transforming E. coli host)
Gene/insert name Mouse Mesp1 cDNA Mouse Myocd
Depositor|Developer DNA Bank, | Nakade, Koji |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution This material is provided with written consent of Developer(nakade@rtc.riken.jp). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

MTAに書く使用条件 事前に樹立者(nakade@rtc.riken.jp)に連絡し、提供承諾を得る。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
MTAに書く付加的使用条件:
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB08495 pAxCA_MsMy Under QC test. Please contact us. DNA solution JPY 32,340 plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pAxCA_MsMy was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08495).

Reference section:

Further references such as user reports and related articles (go to bottom)


QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles