RDB19551 - pBS2SK+IA-ORF2

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pBS2SK+IA-ORF2 (#RDB19551)

Plasmid vector of SARS-CoV2 cDNA encoding ORF2 (Spike protein).

Clone info.	Plasmid vector of SARS-CoV2 cDNA encoding ORF2 (Spike protein). The cDNA was amplified from the genomic RNA of hCoV-19/Japan/NGS-IA-1/2020 (GISAID accession no. EPI-ISL-481251) by RT-PCR and cloned into the SalI site of pBluescript SK(+). Possible RNA synthesis using T7 and T3 RNA polymerase.
Comment	It can be liberated by SalI and SmaI or XbaI double digestion
Vector backbone	pBluescript II SK (+) (plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Ampicillin
Gene/insert name	SARS-CoV-2 ORF2 cDNA
Depositor\|Developer	DNA Bank, \| Nakade, Koji \|

Sequence (full)	RDB19551zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution The RECIPIENT agrees to expressly describe the acknowledgement of the NBRP Human Pathogenic Viruses as the source of the BIOLOGICAL RESOURCE in any publication. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to obtain approval from the Institute of Tropical Medicine, Nagasaki University.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The RECIPIENT agrees to expressly describe the acknowledgement of the NBRP Human Pathogenic Viruses as the source of the BIOLOGICAL RESOURCE in any publication. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to obtain approval from the Institute of Tropical Medicine, Nagasaki University.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	本件リソースを使用して研究成果を発表する場合、NBRPヒト病原ウイルスから提供されたことを明記する。本件リソースを非営利機関が非営利目的の教育・研究用に用いる場合以外は、長崎大学熱帯医学研究所の許諾を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB19551	pBS2SK+IA-ORF2	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBS2SK+IA-ORF2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19551).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB19551_B2C7p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310) Region: T7 pro,insert CDS: 1nt-335nt Sequence file: RDB19551_B2C7a.seq
>19551_B2C7F_2_pUC-M13_-100_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNAN NGCCNGGGTT TTCCCNGTCA CGACGTTGTA 61 AAACGACGGC CAGTGAGCGC GCGTAATACG ACTCACTATA GGGCGAATTG GGTACCGGGC 121 CCCCCCTCGA GGTCGACAAA CGAACAATGT TTGTTTTTCT TGTTTTATTG CCACTAGTCT 181 CTAGTCAGTG TGTTAATCTT ACAACCAGAA CTCAATTACC CCCTGCATAC ACTAATTCTT 241 TCACACGTGG TGTTTATTAC CCTGACAAAG TTTTCAGATC CTCAGTTTTA CATTCAACTC 301 AGGACTTGTT CTTACCTTTC TTTTCCAATG TTACTTGGTT CCATGCTATA CATGTCTCTG 361 GGACCAATGG TACTAAGAGG TTTGATAACC CTGTCCTACC ATTTAATGAT GGTGTTTATT 421 TTGCTTCCAC TGAGAAGTCT AACATAATAA GAGGCTGGAT TTTTGGTACT ACTTTAGATT 481 CNAAGACCCA GTCCCTACTT ATTGTTAATA ACGCTACTAA TGTTGTTATT AAAGTCTGTG 541 AATTTCAATT TTGTAATGAT CCATTTTTGG GTGTTTATTA CCACAAAAAC AACAAAAGTT 601 GGATGGAAAG TGAGTTCAGA GTTTATTCTA GTGCGAATAA TTGCACTTTT GAATATGTCT 661 CTCAGCCTTT TCTTATGGAC CTTGAAGGAA AACAGGGTAA TTTCAAAAAT CTTAGGGAAT 721 TTGTGTTTAA NAATATTGAT GGNTATTTTA AAATATATTC TAAGCACACG CCTATTAATT 781 TAGTGCGTGA TCTCCCTCNN NGTTTTTCGG CTTTAGAACC ATTGGTAGAN TTGCCAATAN 841 GTATTAACNT CACTANGTTT CNAACTTTAC TTGCTTTNCA TANAAGTTAN TTGACTCCTG 901 GTGANTCTTC NTCNNNTGGA NNGCTGGNGN TGCANCTTAN TATGTGGGNT ANCTTCNACC 961 NNNNNTTTTC TNTTAAAATA NAATGNAANT GGNNNCNTTN NNNANGCTGN ANACTGNGNN 1021 NTNGANCNNN NNNNNNAANN NAANNNNNNN NNN //
Primer: pUC-CAPbs_up_f2 (Pr0739) Region: lac pro,lac O,T3 pro,insert CDS: 3822nt-3310nt Sequence file: RDB19551_B2C7b.seq
>19551_B2C7F_2_pUC-CAPbs_up_F2_F02_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCTTTATG CTTCCGGCTC GTATGTTGTG 61 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA 121 GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG 181 CTCTAGAACT AGTGGATCCC CCGGGCTGCA GGAATTCGAT ATCAAGCTTA TCGATACCTT 241 ATGTGTAATG TAATTTGACT CCTTTGAGCA CTGGCTCAGA GTCGTCTTCA TCAAATTTGC 301 AGCAGGATCC ACAAGAACAA CAGCCCTTGA GACAACTACA GCAACTGGTC ATACAGCAAA 361 GCATAATTGT CACCATTACT ATGGCAATCA AGCCAGCTAT AAAACCTAGC CAAATGTACC 421 ATGGCCATTT TATATACTGC TCATACTTTC CAAGTTCTTG GAGATCGATG AGAGATTCAT 481 TTAAATTCTT GGCAACCTCA TTGAGGCGGT CAATTTCTTT TTGAATGTTT ACAACTGAAG 541 CATTAATGCC AGAGATGTCA CCTAAATCAA CATCTGGTGA TGTATGATTC TTAAAATATT 601 TATCTAACTC CTCCTTGAAT GAGTCTAATT CAGGTTGCAA AGGATCATAA ACTGTGTTGT 661 TGACAATTCC TATTACAACA TCACAGTTAC CAGACACAAA TGTGTTGTCT GTAGTAATGA 721 TTTGTGGTTC ATAAAAATTC CTTTGTGTTA CNAACCAGTG TGTGCCATTT GAAACANAGA 781 CNCCTTCACG ANGAAAGTGT GCTTTTCCAT CATGACAAAT GGCANGANCA GTTGTGAAGT 841 TCTTTTCTTG TGCAGGGANN TAANTCACAT GCNANAANAN TACNCCATGA NGNGCTGANT 901 GANGGANNNA NNTNNNNNGA NANCCCTTTN CNCAAAANTC NNCTCTTTTT NANNGNCCNA 961 GNACNCNCTC TGANNNTTTA NNANCANNNA NANNANCNNA ANNTCNNANT NCNGCANNNN 1021 TNNNNNNNNN N //
Primer: SARS-CoV-2_22450R (Pr1168) Region: insert CDS: 826nt-91nt Sequence file: RDB19551_B2C7c.seq
>19551_B2C7F_2_SARS-CoV-2_22450R_D01_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TTTNNTTATA TTTTAATAGG AAAAGTCCTA 61 GGTTGAAGAT AACCCACATA ATAAGCTGCA GCACCAGCTG TCCAACCTGA AGAAGAATCA 121 CCAGGAGTCA AATAACTTCT ATGTAAAGCA AGTAAAGTTT GAAACCTAGT GATGTTAATA 181 CCTATTGGCA AATCTACCAA TGGTTCTAAA GCCGAAAAAC CCTGAGGGAG ATCACGCACT 241 AAATTAATAG GCGTGTGCTT AGAATATATT TTAAAATAAC CATCAATATT CTTAAACACA 301 AATTCCCTAA GATTTTTGAA ATTACCCTGT TTTCCTTCAA GGTCCATAAG AAAAGGCTGA 361 GAGACATATT CAAAAGTGCA ATTATTCGCA CTAGAATAAA CTCTGAACTC ACTTTCCATC 421 CAACTTTTGT TGTTTTTGTG GTAATAAACA CCCAAAAATG GATCATTACA AAATTGAAAT 481 TCACAGACTT TAATAACAAC ATTAGTAGCG TTATTAACAA TAAGTAGGGA CTGGGTCTTC 541 GAATCTAAAG TAGTACCAAA AATCCAGCCT CTTATTATGT TAGACTTCTC AGTGGAAGCA 601 AAATAAACAC CATCATTAAA TGGTAGGACA GGGTTATCAA ACCTCTTAGT ACCATTGGTC 661 CCAGAGACAT GTATAGCATG GAACCAAGTA ACATTGGAAA AGAAAGGTAA GAACAAGTCC 721 TGAGTTGAAT GTAAAACTGA GGATCTGAAA ACTTTGTCAG GGTAATAAAC ACCACGTGTG 781 AAAGANNTAG TGTATGCAGG GGGTAATTGA GTTCTGGNTG TAAGATTAAC ACACTGANTA 841 GAGANTAGTG GCAATAAAAC AAGAAAAANA AACATTGTTC GNTTNNCGAN NNCGAGGGGG 901 GGNCNGGNAC CCANTTCGNC CNATAGTGAG NNNNATTACG CGCGCNNNCN GGNCGNCGTT 961 TTNNANNGTC NNGACNGGNA AANNCCNNNN GTNANCCNAN NTNANNCGCC TNGNANNANA 1021 TCCCCNNTTC GNCNNCTNGN GNNANANCNA ANANNNCCGC NNNNNNNGNC NNNNCCNNCN 1081 NNNNNNNANN NNNNNNGNNN NNNGGNNNNN NNCNNNNNNN NNN //
Primer: SARS-CoV-2_22192F (Pr1153) Region: insert CDS: 691nt-1189nt Sequence file: RDB19551_B2C7d.seq
>19551_B2C7F_2_SARS-CoV-2_22192F_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TNNNNNNNNN TGGTAGANTT GCCNATAGGT 61 ATTAACATCA CTAGGTTTCA AACTTTACTT GCTTTACATA GAAGTTATTT GACTCCTGGT 121 GATTCTTCTT CAGGTTGGAC AGCTGGTGCT GCAGCTTATT ATGTGGGTTA TCTTCAACCT 181 AGGACTTTTC TATTAAAATA TAATGAAAAT GGAACCATTA CAGATGCTGT AGACTGTGCA 241 CTTGACCCTC TCTCAGAAAC AAAGTGTACG TTGAAATCCT TCACTGTAGA AAAAGGAATC 301 TATCAAACTT CTAACTTTAG AGTCCAACCA ACAGAATCTA TTGTTAGATT TCCTAATATT 361 ACAAACTTGT GCCCTTTTGG TGAAGTTTTT AACGCCACCA GATTTGCATC TGTTTATGCT 421 TGGAACAGGA AGAGAATCAG CAACTGTGTT GCTGATTATT CTGTCCTATA TAATTCCGCA 481 TCATTTTCCA CTTTTAAGTG TTATGGAGTG TCTCCTACTA AATTAAATGA TCTCTGCTTT 541 ACTAATGTCT ATGNNNANTC ATTTGTAATT AGAGGTGATG AAGTCAGACA AATCGCTCCA 601 GGGCAAACTG GAAAGATTGC TGATTATAAT TATAAATTAC CAGATGATTT TACAGGCTGC 661 GTTATAGCTT GGAATTCTAA CAATCTTGAT TCTAAGGTTG GTGGTAATTA TAATTACCTG 721 TATAGATTGT TTAGGAAGTC TAATCTCAAA CCTTTNGANA GAGATATTTC NANCTGAAAT 781 CTATCAGGCC GGTAGCACNC CTTGTAATGG TGTTGAANGT NNNNNNTGTT ACTTTCCTTT 841 ANNATCATAT GGNTTCCANC NACTAATGGT GTTGGTTACN ANCNNNNANN GANTANTANT 901 ACTTTCNTTT TGNACTTCTA CATGCNCCAG CNACTGTTTG TGGNANCTAA AANGTCTACT 961 NANTTTNNNT AAAAACNAAN GNGNCNATTN NNNNNNNCNN NNGNTNNACN NNNCAGGNNT 1021 NNTNNCTGAN NNNNNANNAA AANNTTCNGN NNTTNCNNNN ANTTNGNNNN ANNNNNTNNN 1081 NNNNNNN //
Primer: SARS-CoV-2_23.2R (Pr1167) Region: insert CDS: 1598nt-913nt Sequence file: RDB19551_B2C7e.seq
>19551_B2C7F_2_SARS-CoV-2_23.2R_F01_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN NCNTTTGTTT TNNANNNNAA ATTAGTAGAC 61 TTTTTAGGTC CACAAACAGT TGCTGGTGCA TGTAGAAGTT CAAAAGAAAG TACTACTACT 121 CTGTATGGTT GGTAACCAAC ACCATTAGTG GGTTGGAAAC CATATGATTG TAAAGGAAAG 181 TAACAATTAA AACCTTCAAC ACCATTACAA GGTGTGCTAC CGGCCTGATA GATTTCAGTT 241 GAAATATCTC TCTCAAAAGG TTTGAGATTA GACTTCCTAA ACAATCTATA CAGGTAATTA 301 TAATTACCAC CAACCTTAGA ATCAAGATTG TTAGAATTCC AAGCTATAAC GCAGCCTGTA 361 AAATCATCTG GTAATTTATA ATTATAATCA GCAATCTTTC CAGTTTGCCC TGGAGCGATT 421 TGTCTGACTT CATCACCTCT AATTACAAAT GAATCTGCAT AGACATTAGT AAAGCAGAGA 481 TCATTTAATT TAGTAGGAGA CACTCCATAA CACTTAAAAG TGGAAAATGA TGCGGAATTA 541 TATAGGACAG AATAATCAGC AACACAGTTG CTGATTCTCT TCCTGTTCCA AGCATAAACA 601 GATGCAAATC TGGTGGCGTT AAAAACTTCA CCAAAAGGGC ACAAGTTTGT AATATTAGGA 661 AATCTAACAA TAGATTCTGT TGGTTGGACT CTAAAGTTAG AAGTTTGATA GATTCCTTTT 721 TCTACAGTGA AGGANTTCAA CGTACACTTT GTTTCTGAGA GAGGGTCAAG TGCACAGTCN 781 ANNNNNNCTG TANNGNNTCC ATTTTCATTA TATTTTAATA NAAAAGTCCT AGGTTGAANA 841 NAACCNNCAT AATAAGCTGC AGCACCAGCT GTCCNNCCTG NANNANNAAT CNCCAGGAGN 901 CNAATAANTT CTATGTAAAN CNAGTAAAGN TTGAAACCTA NNGATGNNNA TACCTATTGN 961 NAANNCTACC ANNGGTTCTA AAGCCNAAAA NCCTGANGGA NANCACGCNN NANNNANTAG 1021 GNGNGNGNNT ANANNNNNNN TAAAATANNC NTNNATNNTC TTAAANNNNN NNNNCNNNNN 1081 NTTTTNAANT TNNCCNNNTT NNNNTNNNNN NNNNN //
Primer: SARS-CoV-2_23562R (Pr1166) Region: insert CDS: 1956nt-1240nt Sequence file: RDB19551_B2C7f.seq
>19551_B2C7F_2_SARS-CoV-2_23562R_H01_08_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCT NNNNTGAGTT GTTGANNTGN NNNNNCCCTA 61 TTAAACAGCC TGCACGTGTT TGAAAAACAT TAGAACCTGT AGAATAAACA CGCCAAGTAG 121 GAGTAAGTTG ATCTGCATGA ATAGCAACAG GGACTTCTGT GCAGTTAACA TCCTGATAAA 181 GAACAGCAAC CTGGTTAGAA GTATTTGTTC CTGGTGTTAT AACACTGACA CCACCAAAAG 241 AACATGGTGT AATGTCAAGA ATCTCAAGTG TCTGTGGATC ACGGACAGCA TCAGTAGTGT 301 CAGCAATGTC TCTGCCAAAT TGTTGGAAAG GCAGAAACTT TTTGTTAGAC TCAGTAAGAA 361 CACCTGTGCC TGTTAAACCA TTGAAGTTGA AATTGACACA TTTGTTTTTA ACCAAATTAG 421 TAGACTTTTT AGGTCCACAA ACAGTTGCTG GTGCATGTAG AAGTTCAAAA GAAAGTACTA 481 CTACTCTGTA TGGTTGGTAA CCAACACCAT TAGTGGGTTG GAAACCATAT GATTGTAAAG 541 GAAAGTAACA ATTAAAACCT TCAACACCAT TACAAGGTGT GCTACCGGCC TGATAGATTT 601 CAGTTGAAAT ATCTCTCTCA AAAGGTTTGA GATTAGACTT CCTAAACAAT CTATACAGGT 661 AATTATAATT ACCACCAACC TTAGAATCAA GATTGTTAGA ATTCCAAGCT ATAACGCAGC 721 CTGTAAAATC ATCTGGTAAT TTATAATTAT AATCAGCAAT CTTTCCAGTT TGNCCTGNAG 781 CGATTTGTCT GACTTCNTCA CCTCTAATTA CAAATGAATC TGCATAGACA TTAGTAAAGC 841 AGAGATCATT TAATTTAGTA NGAGACACTC CNTAACACTT AAAAGTGGAA ANNGATGCGG 901 AATTATATAG GANAGAATAN TCANCNACAC AGTTGCTGAN TCTCNNNCTG NNNNNAGCAT 961 NAACNGANGN AANTCTGGTG NNGNTAAAAN CNTCNNCNAA ANGGNNNAAG TTTNNNNNAN 1021 TNNNAAATCN ANCNNNANNN TNNGNTNGNT NNGNNNNNNA NNNNNNAANT NGNNANANTN 1081 CNTTTTTCNN NNNNNNNN //
Primer: SARS-CoV-2_24240R (Pr1169) Region: insert CDS: 2600nt-1827nt Sequence file: RDB19551_B2C7g.seq
>19551_B2C7F_2_SARS-CoV-2_24240R_B02_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGNNNNNNG TGCNNAAGTG TATTGAGCAA TCATTTCNTC 61 TGTGAGCAAA GGTGGCAAAA CAGTAAGGCC GTTAAACTTT TGTGCACAAA TGAGGTCTCT 121 AGCAGCAATA TCACCAAGGC AATCACCATA TTGTTTGATG AAGCCAGCAT CTGCAAGTGT 181 CACTTTGTTG AAAAGTAGAT CTTCAATAAA TGACCTCTTG CTTGGTTTTG ATGGATCTGG 241 TAATATTTGT GAAAAATTAA AACCACCAAA ATCTTTAATT GGTGGTGTTT TGTAAATTTG 301 TTTGACTTGT GCAAAAACTT CTTGGGTGTT TTTGTCTTGT TCAACAGCTA TTCCAGTTAA 361 AGCACGGTTT AATTGTGTAC AAAAACTGCC ATATTGCAAC AAAAGATTGC TGCATTCAGT 421 TGAATCACCA CAAATGTACA TTGTACAATC TACTGATGTC TTGGTCATAG ACACTGGTAG 481 AATTTCTGTG GTAACACTAA TAGTAAAATT TGTGGGTATG GCAATAGAGT TATTAGAGTA 541 AGCAACTGAA TTTTCTGCAC CAAGTGACAT AGTGTAGGCA ATGATGGATT GACTAGCTAC 601 ACTACGTGCC CGCCGAGGAG AATTAGTCTG AGTCTGATAA CTAGCGCATA TACCTGCACC 661 AATGGGTATG TCACACTCAT ATGAGTTGTT GACATGTTCA GCCCCTATTA AACAGCCTGC 721 ACGTGTTTGA AAAACATTAG AACCTGTAGA ATAAACACGC CAAGTAGGAG TAAGTTGATC 781 TGCATGAATA GCAACAGGGA CTTCTGTGCA GTTAACATCC TGATAAAGAA CANCAACCTG 841 NTTAGAAGTA TTTGTTCCTG NTGTTATAAC NCTGACACCA CNNAAAGAAN NTGGNGTAAT 901 GTCAAGAATC TCAAGTGTCT GNGNATNANG GANAGCATNA NTANTGTCAN CANTGNCTCT 961 GNNAANNGNN NGNAANNNNN NNNNTTTTTG NTNNNNTCNN NNNNNNNN //
Primer: SARS-CoV-2_24001F (Pr1156) Region: insert CDS: 2501nt-3225nt Sequence file: RDB19551_B2C7h.seq
>19551_B2C7F_2_SARS-CoV-2_24001F_A02_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNT NNNNNNAAGT GNNNCTTGCA GATGCTGGCT TCNTCAAACA 61 ATATGGTGAT TGCCTTGGTG ATATTGCTGC TAGAGACCTC ATTTGTGCAC AAAAGTTTAA 121 CGGCCTTACT GTTTTGCCAC CTTTGCTCAC AGATGAAATG ATTGCTCAAT ACACTTCTGC 181 ACTGTTAGCG GGTACAATCA CTTCTGGTTG GACCTTTGGT GCAGGTGCTG CATTACAAAT 241 ACCATTTGCT ATGCAAATGG CTTATAGGTT TAATGGTATT GGAGTTACAC AGAATGTTCT 301 CTATGAGAAC CAAAAATTGA TTGCCAACCA ATTTAATAGT GCTATTGGCA AAATTCAAGA 361 CTCACTTTCT TCCACAGCAA GTGCACTTGG AAAACTTCAA GATGTGGTCA ACCAAAATGC 421 ACAAGCTTTA AACACGCTTG TTAAACAACT TAGCTCCAAT TTTGGTGCAA TTTCAAGTGT 481 TTTAAATGAT ATCCTTTCAC GTCTTGACAA AGTTGAGGCT GAAGTGCAAA TTGATAGGTT 541 GATCACAGGC AGACTTCAAA GTTTGCAGAC ATATGTGACT CAACAATTAA TTAGAGCTGC 601 AGAAATCAGA GCTTCTGCTA ATCTTGCTGC TACTAAAATG TCAGAGTGTG TACTTGGACA 661 ATCAAAAAGA GTTGATTTTT GTGGAAAGGG CTATCATCTT ATGTCCTTCC CTCAGTCAGC 721 ACCTCATGGT GTAGTCTTCT TGCATGTGAC TTATGTCCCT GCACAAGAAA AGAACTTCNC 781 AACTGCTCCT GCCATTTGTC ATGATGGAAA AGCACACTTT CCTCGTGAAN GTGTCTTTGT 841 TTCAAATGNC ACACACTGNT TTGTAACACA AAGGAATTTT TATGAACCAC NAATCATTAC 901 TACAGANAAC ACATTTGTGT CTGGNANNTG TGANGTTGTA ANNNNNATTG TCNNCNACNC 961 AGTTTANNAT CCTTTGCNNC CTGAATTANN NNCNTTCNNN NNNNANNNAG NNAANNATTT 1021 TNNNNNNCAT ACNTCNNNNN NNNNN //
Primer: SARS-CoV-2_24599F (Pr1157) Region: insert CDS: 3080nt-3785nt Sequence file: RDB19551_B2C7i.seq
>19551_B2C7F_2_SARS-CoV-2_24599F_C02_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CTTGCTGCTN CTAAAATGTC AGAGTGTGTA 61 CTTGGACAAT CAAAAAGAGT TGATTTTTGT GGAAAGGGCT ATCATCTTAT GTCCTTCCCT 121 CAGTCAGCAC CTCATGGTGT AGTCTTCTTG CATGTGACTT ATGTCCCTGC ACAAGAAAAG 181 AACTTCACAA CTGCTCCTGC CATTTGTCAT GATGGAAAAG CACACTTTCC TCGTGAAGGT 241 GTCTTTGTTT CAAATGGCAC ACACTGGTTT GTAACACAAA GGAATTTTTA TGAACCACAA 301 ATCATTACTA CAGACAACAC ATTTGTGTCT GGTAACTGTG ATGTTGTAAT AGGAATTGTC 361 AACAACACAG TTTATGATCC TTTGCAACCT GAATTAGACT CATTCAAGGA GGAGTTAGAT 421 AAATATTTTA AGAATCATAC ATCACCAGAT GTTGATTTAG GTGACATCTC TGGCATTAAT 481 GCTTCAGTTG TAAACATTCA AAAAGAAATT GACCGCCTCA ATGAGGTTGC CAAGAATTTA 541 AATGAATCTC TCATCGATCT CCAAGAACTT GGAAAGTATG AGCAGTATAT AAAATGGCCA 601 TGGTACATTT GGCTAGGTTT TATAGCTGGC TTGATTGCCA TAGTAATGGT GACAATTATG 661 CTTTGCTGTA TGACCAGTTG CTGTAGTTGT CTCAAGGGCT GTTGTTCTTG TGGATCCTGC 721 TGCAAATTTG ATGAAGACGA CTCTGANCCA GTGCTCAAAG GAGTCAAATT ACATTACACA 781 TAAGGTATCG ATAAGCTTGA TATCGAATTC CTGCAGCCCG GGGGANCCAC TAGTTCTAGA 841 GCGGCCGCCA CCGCGGNGGA NCTCCAGCTT TTGTTCCCTT TAGTGANGGN TAANTNGCGC 901 GCTTGNNGNA ATCATGGTCA NAGCTGTTNN CNGNGTNNAA TTGNTATCCG CTCNCANTTN 961 CNCACNANNT ACNANCCNGA NNCATAAAGN GNNAAAGNCN GGGGGNGNCT NNNGANTGAN 1021 CNNACNNNNN NTANTNGCGT NGNNNNNNNN GNCNNNCTTT NCAGTNNGNA ANNNNNNNNN 1081 N //