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pUCtk2x2

Universal donor vector for double-tk CRISPR/Cas9 knock-in system. Kpn I/Asp718I site is available for insertion of homology arm,

Catalog number RDB19569
Resource name pUCtk2x2
Alternative name pUCtk2x2N3
Clone info. Universal donor vector for double-tk CRISPR/Cas9 knock-in system. Kpn I/Asp718I site is available for insertion of homology arm,
Vector backbone pUC19 (plasmid)
Gene/insert name Herpes simplex virus (HSV) thymidin kinase (TK) gene.
Depositor|Developer DNA Bank, | Nakade, Koji |
 
Sequence (full) RDB19569hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19569_B3Chp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.　(Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))。
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RDB19569 pUCtk2x2 DNA solution

How to cite this biological resource

Materials & Methods section:

The pUCtk2x2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19569).

Reference section:

Nakade, K., Tsukamoto, S., Nakashima, K., An, Y., Sato, I., Li, J., Shimoda, Y., Hemmi, Y., Miwa, Y., Hayashi, Y., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]