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pUCtk2x2 (#RDB19569)

Universal donor vector for double-tk CRISPR/Cas9 knock-in system. Kpn I/Asp718I site is available for insertion of homology arm,


Drawn by SnapGene® software
Sequence (full) RDB19569hts01.seq
Publication Nakade, K., Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]
Test sheet RDB19569_B3Chp1-1.pdf 
Assembled from experimentally sequenced data.
 
Alternative name pUCtk2x2N3
Clone info. Universal donor vector for double-tk CRISPR/Cas9 knock-in system. Kpn I/Asp718I site is available for insertion of homology arm,
Vector backbone pUC19 (plasmid)
Gene/insert name Herpes simplex virus (HSV) thymidin kinase (TK) gene.
Depositor|Developer DNA Bank, | Nakade, Koji |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.　(Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))
提供案内 (日本国内) [open/close]

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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))。

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RDB19569 pUCtk2x2 DNA solution

How to cite this biological resource

Materials & Methods section:

The pUCtk2x2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19569).

Reference section:

Nakade, K., Tsukamoto, S., Nakashima, K., An, Y., Sato, I., Li, J., Shimoda, Y., Hemmi, Y., Miwa, Y., Hayashi, Y., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]
protocol Hayashi, Y., Generation of Reporter Human Pluripotent Stem Cells Using CRISPR/Cas9 Editing. In: Ding, B., Tang, Y. (eds) Human Induced Pluripotent Stem Cells. Neuromethods, vol 210. Humana, New York, NY (2024). [link to Journal]
user_report Takagi, D., Generation of MBP-tdTomato reporter human induced pluripotent stem cell line for live myelin visualization. Stem Cell Res. 79: 103493 (2024). PMID 39032428. [link to RRC of NBRP]