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Kenichi Nakashima's Resource

Kenichi Nakashima's web page in English /  in Japanese.

Learn more about their research, in English  /  in Japanese (by Reseachmap[link])

Catalog #Name of cloneRunning titlePublication
RDB19573pUC wLG3-G-EZ(ERV) Genome editing tool to tag EGFP at C-termini of target protein. Containing -LG3linker-GFP tag and EF1promoter-BleoR. Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB19574pUC wLG3-T-EZ(ERV) Genome editing tool to tag tdTomato at C-termini of target protein. Containing -LG3linker-GFP tag and EF1promoter-BleoR. Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB19575pUlrTKGenome editting tool to select aimed integration efficiently. The platform of integrative donor fragment containing acouple of tk, sucide gene, & 5UAS Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB19576pUlrTK H2BC21stp-armsGenome editing tool to subclone tagging fragment on human histone H2BC21, C-terminaly.Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB19577pUlrTK H2BC21-G-EZGenome editing tool. Donner vector to tag EGFP at human histon H2BC21, C-terminaly. Selection with Zeo & GCVNakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB19578pUlrTK H2BC21-T-EZGenome editing tool. Donner vector to tag tdTomato at human histon H2BC21, C-terminaly. Selection with Zeo & GCVNakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB19579pX330_CDM45-1Genome editing tool. sgRNA and SpCas9 expression in one vector. Subcloned an oligo nucleotide to target human H2BC21 gene stop codon nearby.Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB19580pX330_CDM24-1Genome editing tool. sgRNA and SpCas9 expression in one vector. Subcloned an oligo nucleotide to target human POU5F1 gene stop codon nearby.Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB20120pUCtk2x2_MYH7-TEZGenome editing tool. Donner vector to tag 2A-tdTomato at human MYH7, C-terminaly. Selection with Zeo & GCV.Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB20121pX330_gMYH7sgRNA to target human MYH7 gene stop codon nearby and SpCas9 expression in one vector.Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]

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2024.06.15

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