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Kenichi Nakashima's web page in English / in Japanese
| Catalog # | Name of clone | Running title | Publication |
| RDB20462 | pCRtk_SOX10-TEZ | Donner vector to tag T2A-tdTomato at human SOX10, C-terminaly. | Unpublished bioresource |
| RDB20461 | pX330_SOX10 | Genome editing tool to target human SOX10 gene stop codon nearby. | Unpublished bioresource |
| RDB20282 | pCRtk-EEF1A1-iRFP670-BSD | Genome editing tool. Donner vector to tag T2A-tandem-humanized iRFP670-T2A-BSD at human EEF1A1. | Wakimoto, A., Intraplacental injection of human iPSC-derived PDX1+ pancreatic progenitors prolongs Pdx1-deficient mice survival. bioRxiv doi: 10.1101/2024.05.09.593461 (2024). (original). |
| RDB20281 | pUCtk-PDX1-TbsdEZ | Genome editing tool. Donner vector to tag T2A-tdTomato-T2A-BSD at human PDX1. | Wakimoto, A., Intraplacental injection of human iPSC-derived PDX1+ pancreatic progenitors prolongs Pdx1-deficient mice survival. bioRxiv doi: 10.1101/2024.05.09.593461 (2024). (original). |
| RDB20280 | pX330-PDX1 | Genome editing tool. sgRNA and SpCas9 expression in one vector to target human PDX1 gene stop codon nearby. | Wakimoto, A., Intraplacental injection of human iPSC-derived PDX1+ pancreatic progenitors prolongs Pdx1-deficient mice survival. bioRxiv doi: 10.1101/2024.05.09.593461 (2024). (original). |
| RDB20258 | pcmvC5U-nsp1deltaRB-pA | Mammalian expression vector of SARS-CoV-2_5'UTR-nsp1 deltaGlu 155-His165: ribosome binding site for disrupting regular translation. | Unpublished bioresource |
| RDB20257 | pcmvC5U-NSP1-pA | Mammalian expression vector of SARS-CoV-2_5'UTR-NSP1. | Unpublished bioresource |
| RDB20256 | pcmvT7-nsp1deltaRB-pA | Mammalian expression and T7 in vitro transcription dual vector of SARS-CoV-2 nsp1deltaGlu155-His165: ribosome binding site for disrupting regular translation. | Unpublished bioresource |
| RDB20255 | pcmvT7-NSP1-pA | Mammalian expression and T7 in vitro transcription dual vector of SARS-CoV-2 NSP1. | Unpublished bioresource |
| RDB20254 | pcmvC5U-KpnI-polyA | Infusion/Gibson assembly platform vector for mammalian expression mRNA with SARS-CoV2-5' UTR. | Unpublished bioresource |
| RDB20253 | pMWcmvT7-ORF1a(nsp1deltaRB)-pA | Mammalian expression and T7 in vitro transcription dual ussage vector of SARS-CoV-2 ORF1a (nsp1 deltaGlu 155-His165: ribosome binding site for disrupting regular translation). | Unpublished bioresource |
| RDB20121 | pX330_gMYH7 | sgRNA to target human MYH7 gene stop codon nearby and SpCas9 expression in one vector. | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
| RDB20120 | pUCtk2x2_MYH7-TEZ | Genome editing tool. Donner vector to tag 2A-tdTomato at human MYH7, C-terminaly. Selection with Zeo & GCV. | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
| RDB19690 | pT7-CoV-S(W152L E484K D614G G769V)-pA | mammalian expression and T7 In Vitro Transcription dual vector of SARS-CoV-2 Spike(W152L E484K D614G G769V). Bacterial high copy number plasmid. | Unpublished bioresource |
| RDB19689 | pT7-CoV-S(D614G)-pA | mammalian expression and T7 In Vitro Transcription dual vector of SARS-CoV-2 Spike(D614G). Bacterial high copy number plasmid. | Unpublished bioresource |
| RDB19688 | pT7-CoV-S-pA | mammalian expression and T7 In Vitro Transcription dual vector of SARS-CoV-2 Spike. Bacterial high copy number plasmid. | Unpublished bioresource |
| RDB19687 | pMWT7-CoV-ORF1ab-pA | Mammalian expression and T7 In Vitro Transcription dual vector of SARS-CoV-2 ORF1ab. Bacterial low copy number plasmid. | Unpublished bioresource |
| RDB19686 | pMWT7-CoV-ORF1a-pA | mammalian expression and T7 In Vitro Transcription dual ussage vector of SARS-CoV-2 ORF1a. Bacterial low copy number plasmid. | Unpublished bioresource |
| RDB19685 | pMWT7-CoV-ORF1a(IA)-pA | mammalian expression and T7 In Vitro Transcription dual ussage vector of SARS-CoV-2 ORF1a(IA strain). Bacterial low copy number plasmid. | Unpublished bioresource |
| RDB19684 | pMWcmvT7-KpnI-pA | Infusion/Gibson assembly platform vector for dual ussage mammalian expression and T7 In Vitro Transcription. Bacterial strictly low copy number plasmid (pMW219 backbone). | Unpublished bioresource |
| RDB19683 | pBRcmvT7-KpnI-pA | Infusion/Gibson assembly platform vectorfor dual ussage mammalian expression and T7 In Vitro Transcription. Bacterial relatively low copy number plasmid (pBR322 backbone). Tetracyclin resistance gene has been truncated. | Unpublished bioresource |
| RDB19682 | pcmvT7-KpnI-polyA | Infusion/Gibson assembly platform vector for dual ussage mammalian expression and T7 In Vitro Transcription. Bacterial high copy number plasmid (pCDNA3 backbone). | Unpublished bioresource |
| RDB19580 | pX330_CDM24-1 | Genome editing tool. sgRNA and SpCas9 expression in one vector. Subcloned an oligo nucleotide to target human POU5F1 gene stop codon nearby. | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
| RDB19579 | pX330_CDM45-1 | Genome editing tool. sgRNA and SpCas9 expression in one vector. Subcloned an oligo nucleotide to target human H2BC21 gene stop codon nearby. | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
| RDB19578 | pUlrTK H2BC21-T-EZ | Genome editing tool. Donner vector to tag tdTomato at human histon H2BC21, C-terminaly. Selection with Zeo & GCV | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
| RDB19577 | pUlrTK H2BC21-G-EZ | Genome editing tool. Donner vector to tag EGFP at human histon H2BC21, C-terminaly. Selection with Zeo & GCV | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
| RDB19576 | pUlrTK H2BC21stp-arms | Genome editing tool to subclone tagging fragment on human histone H2BC21, C-terminaly. | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
| RDB19575 | pUlrTK | Genome editting tool to select aimed integration efficiently. The platform of integrative donor fragment containing acouple of tk, sucide gene, & 5UAS | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
| RDB19574 | pUC wLG3-T-EZ(ERV) | Genome editing tool to tag tdTomato at C-termini of target protein. Containing -LG3linker-GFP tag and EF1promoter-BleoR. | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
| RDB19573 | pUC wLG3-G-EZ(ERV) | Genome editing tool to tag EGFP at C-termini of target protein. Containing -LG3linker-GFP tag and EF1promoter-BleoR. | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
2025.03.17
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