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Kenichi Nakashima's web page in English / in Japanese.
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Catalog # | Name of clone | Running title | Publication |
RDB20121 | pX330_gMYH7 | sgRNA to target human MYH7 gene stop codon nearby and SpCas9 expression in one vector. | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
RDB20120 | pUCtk2x2_MYH7-TEZ | Genome editing tool. Donner vector to tag 2A-tdTomato at human MYH7, C-terminaly. Selection with Zeo & GCV. | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
RDB19580 | pX330_CDM24-1 | Genome editing tool. sgRNA and SpCas9 expression in one vector. Subcloned an oligo nucleotide to target human POU5F1 gene stop codon nearby. | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
RDB19579 | pX330_CDM45-1 | Genome editing tool. sgRNA and SpCas9 expression in one vector. Subcloned an oligo nucleotide to target human H2BC21 gene stop codon nearby. | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
RDB19578 | pUlrTK H2BC21-T-EZ | Genome editing tool. Donner vector to tag tdTomato at human histon H2BC21, C-terminaly. Selection with Zeo & GCV | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
RDB19577 | pUlrTK H2BC21-G-EZ | Genome editing tool. Donner vector to tag EGFP at human histon H2BC21, C-terminaly. Selection with Zeo & GCV | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
RDB19576 | pUlrTK H2BC21stp-arms | Genome editing tool to subclone tagging fragment on human histone H2BC21, C-terminaly. | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
RDB19575 | pUlrTK | Genome editting tool to select aimed integration efficiently. The platform of integrative donor fragment containing acouple of tk, sucide gene, & 5UAS | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
RDB19574 | pUC wLG3-T-EZ(ERV) | Genome editing tool to tag tdTomato at C-termini of target protein. Containing -LG3linker-GFP tag and EF1promoter-BleoR. | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
RDB19573 | pUC wLG3-G-EZ(ERV) | Genome editing tool to tag EGFP at C-termini of target protein. Containing -LG3linker-GFP tag and EF1promoter-BleoR. | Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP] |
2024.10.04
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