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Kenichi Nakashima's Resource

Kenichi Nakashima's web page in English /  in Japanese

Catalog #Name of cloneRunning titlePublication
RDB20462pCRtk_SOX10-TEZDonner vector to tag T2A-tdTomato at human SOX10, C-terminaly.Unpublished bioresource
RDB20461pX330_SOX10Genome editing tool to target human SOX10 gene stop codon nearby.Unpublished bioresource
RDB20282pCRtk-EEF1A1-iRFP670-BSDGenome editing tool. Donner vector to tag T2A-tandem-humanized iRFP670-T2A-BSD at human EEF1A1.Wakimoto, A., Intraplacental injection of human iPSC-derived PDX1+ pancreatic progenitors prolongs Pdx1-deficient mice survival. bioRxiv doi: 10.1101/2024.05.09.593461 (2024). (original).
RDB20281pUCtk-PDX1-TbsdEZGenome editing tool. Donner vector to tag T2A-tdTomato-T2A-BSD at human PDX1.Wakimoto, A., Intraplacental injection of human iPSC-derived PDX1+ pancreatic progenitors prolongs Pdx1-deficient mice survival. bioRxiv doi: 10.1101/2024.05.09.593461 (2024). (original).
RDB20280pX330-PDX1Genome editing tool. sgRNA and SpCas9 expression in one vector to target human PDX1 gene stop codon nearby.Wakimoto, A., Intraplacental injection of human iPSC-derived PDX1+ pancreatic progenitors prolongs Pdx1-deficient mice survival. bioRxiv doi: 10.1101/2024.05.09.593461 (2024). (original).
RDB20258pcmvC5U-nsp1deltaRB-pAMammalian expression vector of SARS-CoV-2_5'UTR-nsp1 deltaGlu 155-His165: ribosome binding site for disrupting regular translation.Unpublished bioresource
RDB20257pcmvC5U-NSP1-pAMammalian expression vector of SARS-CoV-2_5'UTR-NSP1.Unpublished bioresource
RDB20256pcmvT7-nsp1deltaRB-pAMammalian expression and T7 in vitro transcription dual vector of SARS-CoV-2 nsp1deltaGlu155-His165: ribosome binding site for disrupting regular translation.Unpublished bioresource
RDB20255pcmvT7-NSP1-pAMammalian expression and T7 in vitro transcription dual vector of SARS-CoV-2 NSP1.Unpublished bioresource
RDB20254pcmvC5U-KpnI-polyAInfusion/Gibson assembly platform vector for mammalian expression mRNA with SARS-CoV2-5' UTR.Unpublished bioresource
RDB20253pMWcmvT7-ORF1a(nsp1deltaRB)-pAMammalian expression and T7 in vitro transcription dual ussage vector of SARS-CoV-2 ORF1a (nsp1 deltaGlu 155-His165: ribosome binding site for disrupting regular translation).Unpublished bioresource
RDB20121pX330_gMYH7sgRNA to target human MYH7 gene stop codon nearby and SpCas9 expression in one vector.Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB20120pUCtk2x2_MYH7-TEZGenome editing tool. Donner vector to tag 2A-tdTomato at human MYH7, C-terminaly. Selection with Zeo & GCV.Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB19690pT7-CoV-S(W152L E484K D614G G769V)-pAmammalian expression and T7 In Vitro Transcription dual vector of SARS-CoV-2 Spike(W152L E484K D614G G769V). Bacterial high copy number plasmid.Unpublished bioresource
RDB19689pT7-CoV-S(D614G)-pAmammalian expression and T7 In Vitro Transcription dual vector of SARS-CoV-2 Spike(D614G). Bacterial high copy number plasmid.Unpublished bioresource
RDB19688pT7-CoV-S-pAmammalian expression and T7 In Vitro Transcription dual vector of SARS-CoV-2 Spike. Bacterial high copy number plasmid.Unpublished bioresource
RDB19687pMWT7-CoV-ORF1ab-pAMammalian expression and T7 In Vitro Transcription dual vector of SARS-CoV-2 ORF1ab. Bacterial low copy number plasmid.Unpublished bioresource
RDB19686pMWT7-CoV-ORF1a-pAmammalian expression and T7 In Vitro Transcription dual ussage vector of SARS-CoV-2 ORF1a. Bacterial low copy number plasmid.Unpublished bioresource
RDB19685pMWT7-CoV-ORF1a(IA)-pA mammalian expression and T7 In Vitro Transcription dual ussage vector of SARS-CoV-2 ORF1a(IA strain). Bacterial low copy number plasmid.Unpublished bioresource
RDB19684pMWcmvT7-KpnI-pAInfusion/Gibson assembly platform vector for dual ussage mammalian expression and T7 In Vitro Transcription. Bacterial strictly low copy number plasmid (pMW219 backbone).Unpublished bioresource
RDB19683pBRcmvT7-KpnI-pAInfusion/Gibson assembly platform vectorfor dual ussage mammalian expression and T7 In Vitro Transcription. Bacterial relatively low copy number plasmid (pBR322 backbone). Tetracyclin resistance gene has been truncated.Unpublished bioresource
RDB19682pcmvT7-KpnI-polyAInfusion/Gibson assembly platform vector for dual ussage mammalian expression and T7 In Vitro Transcription. Bacterial high copy number plasmid (pCDNA3 backbone).Unpublished bioresource
RDB19580pX330_CDM24-1Genome editing tool. sgRNA and SpCas9 expression in one vector. Subcloned an oligo nucleotide to target human POU5F1 gene stop codon nearby.Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB19579pX330_CDM45-1Genome editing tool. sgRNA and SpCas9 expression in one vector. Subcloned an oligo nucleotide to target human H2BC21 gene stop codon nearby.Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB19578pUlrTK H2BC21-T-EZGenome editing tool. Donner vector to tag tdTomato at human histon H2BC21, C-terminaly. Selection with Zeo & GCVNakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB19577pUlrTK H2BC21-G-EZGenome editing tool. Donner vector to tag EGFP at human histon H2BC21, C-terminaly. Selection with Zeo & GCVNakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB19576pUlrTK H2BC21stp-armsGenome editing tool to subclone tagging fragment on human histone H2BC21, C-terminaly.Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB19575pUlrTKGenome editting tool to select aimed integration efficiently. The platform of integrative donor fragment containing acouple of tk, sucide gene, & 5UAS Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB19574pUC wLG3-T-EZ(ERV) Genome editing tool to tag tdTomato at C-termini of target protein. Containing -LG3linker-GFP tag and EF1promoter-BleoR. Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]
RDB19573pUC wLG3-G-EZ(ERV) Genome editing tool to tag EGFP at C-termini of target protein. Containing -LG3linker-GFP tag and EF1promoter-BleoR. Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. (original). [link to RRC of NBRP]

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2025.03.17

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