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pUC wLG3-G-EZ(ERV) (#RDB19573)

Genome editing tool to tag EGFP at C-termini of target protein. Containing -LG3linker-GFP tag and EF1promoter-BleoR.


Drawn by SnapGene® software
Sequence (full) RDB19573hts01.seq
Publication Nakade, K., Cell Rep. Methods 3 (12): 100662 (2023). [link to RRC of NBRP]
Test sheet RDB19573_B2Ddp1-1.pdf 
Assembled from experimentally sequenced data.
 
Alternative name pUC-2xLG3-GEZ
Clone info. Genome editing tool to tag EGFP at C-termini of target protein. Containing -LG3linker-GFP tag and EF1promoter-BleoR.
Vector backbone pUC19 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Aequorea victoria GFP (EGFP) cDNA.
Depositor|Developer DNA Bank, | Nakashima, Kenichi |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.　(Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))
提供案内 (日本国内) [open/close]

必要書類
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))。

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RDB19573 pUC wLG3-G-EZ(ERV) DNA solution

How to cite this biological resource

Materials & Methods section:

The pUC wLG3-G-EZ(ERV) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19573).

Reference section:

Nakade, K., Tsukamoto, S., Nakashima, K., An, Y., Sato, I., Li, J., Shimoda, Y., Hemmi, Y., Miwa, Y., Hayashi, Y., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]