Resource data sheet
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pUC wLG3-G-EZ(ERV) (#RDB19573)

Genome editing tool to tag EGFP at C-termini of target protein. Containing -LG3linker-GFP tag and EF1promoter-BleoR.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Nakade, K., Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name pUC-2xLG3-GEZ
Clone info. Genome editing tool to tag EGFP at C-termini of target protein. Containing -LG3linker-GFP tag and EF1promoter-BleoR.
Vector backbone pUC19 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Growth conditions LB+Amp, 37℃
Gene/insert name Aequorea victoria GFP (EGFP) cDNA.
Depositor|Developer DNA Bank, | Nakashima, Kenichi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.　(Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))
Additional terms and conditions:
Further transfer, provision of services or resale of the Zeocin resistance gene, and the Deposited Materials is prohibited without InvivoGen's prior written consent. In addition, InvivoGen’s Limited Use Label License applies to your use of this product.
Remarks
Zeocin® is a trademark of InvivoGen registered with the United States Patent and Trademark Office and foreign equivalents. See InvivoGen Limited Use Label License.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))。
付加的使用条件：
InvivoGen社の事前の書面による同意がない限り、ゼオシン耐性遺伝子および寄託マテリアルの第三者への移転、サービスの提供、または再販売は禁止されています。また、ご利用に際してInvivoGen社のLimited Use Label Licenseが適用されます。
備考
ZeocinはInvivoGen社の製品です。Zeocin®は、米国特許商標庁および米国外の同等機関に登録されたInvivoGen社の商標です。InvivoGen Limited Use Label Licenseをご確認ください。

Catalog # Resource name Shipping form Fee
RDB19573 pUC wLG3-G-EZ(ERV) DNA solution

How to cite this biological resource

Materials & Methods section:

The pUC wLG3-G-EZ(ERV) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19573).

Reference section:

Nakade, K., Tsukamoto, S., Nakashima, K., An, Y., Sato, I., Li, J., Shimoda, Y., Hemmi, Y., Miwa, Y., Hayashi, Y., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [PubMed] [Article] [RRC of NBRP]