Resource data sheet
DNA Bank Top

pMWcmvT7-ORF1a(nsp1deltaRB)-pA (#RDB20253)

Mammalian expression and T7 in vitro transcription dual ussage vector of SARS-CoV-2 ORF1a (nsp1 deltaGlu 155-His165: ribosome binding site for disrupting regular translation).


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Clone info. Mammalian expression and T7 in vitro transcription dual ussage vector of SARS-CoV-2 ORF1a (nsp1 deltaGlu 155-His165: ribosome binding site for disrupting regular translation). Corresponding NC_045512.2: 266..13483, Bacterial low copy number plasmid.
Comment Delivative of pMWT7-KpnI-polyA (RDB19684).
Vector backbone pMWcmvT7-KpnI-polyA (RDB19684) (plasmid)
Growth conditions LB+km, 23℃
Gene/insert name SARS-CoV-2 ORF1a
Depositor|Developer DNA Bank, | Nakashima, Kenichi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. ()
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用すること(決定次第、理研BRCで記載する)。

Catalog # Resource name Shipping form Fee
RDB20253 pMWcmvT7-ORF1a(nsp1deltaRB)-pA DNA solution

How to cite this biological resource

Materials & Methods section:

The pMWcmvT7-ORF1a(nsp1deltaRB)-pA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20253).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles