Resource data sheet
DNA Bank Top
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pMWcmvT7-ORF1a(nsp1deltaRB)-pA (#RDB20253)

Mammalian expression and T7 in vitro transcription dual ussage vector of SARS-CoV-2 ORF1a (nsp1 deltaGlu 155-His165: ribosome binding site for disrupting regular translation).

Clone info. Mammalian expression and T7 in vitro transcription dual ussage vector of SARS-CoV-2 ORF1a (nsp1 deltaGlu 155-His165: ribosome binding site for disrupting regular translation). Corresponding NC_045512.2: 266..13483, Bacterial low copy number plasmid.
Comment Delivative of pMWT7-KpnI-polyA (RDB19684).
Vector backbone pMWcmvT7-KpnI-polyA (RDB19684) (plasmid)
Growth conditions LB+km, 23℃
Gene/insert name SARS-CoV-2 ORF1a
Depositor|Developer DNA Bank, | Nakashima, Kenichi |
 
Sequence (full) RDB20253hts01.seq checkAssembled from experimentally sequenced data.
Publication Unpublished bioresource
Test sheet RDB20253C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. ()
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用すること(決定次第、理研BRCで記載する)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20253 pMWcmvT7-ORF1a(nsp1deltaRB)-pA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pMWcmvT7-ORF1a(nsp1deltaRB)-pA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20253).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles