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pX330_gMYH7 (#RDB20121)

sgRNA to target human MYH7 gene stop codon nearby and SpCas9 expression in one vector.

Clone info. Genome editing tool. sgRNA and SpCas9 expression in one vector. Subcloned 5'-tgttacacaggctccagcat-3' in between BbsI sites to target human MYH7 gene stop codon nearby.
Vector backbone pX330 (plasmid)
Gene/insert name synthetic DNA targeting human MYH7 3'UTR.
Depositor|Developer DNA Bank, | Nakashima, Kenichi |
 
Sequence (full) RDB20121hts01.seq checkAssembled from experimentally sequenced data.
Publication Nakade, K., Cell Rep. Methods 3 (12): 100662 (2023). [link to RRC of NBRP]
Test sheet RDB20121_B3Jvp1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.　(Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20121 pX330_gMYH7 DNA solution

How to cite this biological resource

Materials & Methods section:

The pX330_gMYH7 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20121).

Reference section:

Nakade, K., Tsukamoto, S., Nakashima, K., An, Y., Sato, I., Li, J., Shimoda, Y., Hemmi, Y., Miwa, Y., Hayashi, Y., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]