Please review the QC test results indicated by icon below as well as clone information before placing your order.

pUlrTK (#RDB19575)

Genome editting tool to select aimed integration efficiently. The platform of integrative donor fragment containing acouple of tk, sucide gene, & 5UAS

Alternative name	pUCtk2x2-UAS, pwTK5UAS
Clone info.	Genome editing tool to select aimed integration efficiently. The platform of integrative donor fragment containing a couple of tk, suicide gene, & 5UAS.
Vector backbone	pUC19 (plasmid)
Size of vector backbone	2.7 kb
Selectable markers	Ampicillin
Gene/insert name	HSV tk 5' UTR, coding region, poly(A) signal.
Depositor|Developer	DNA Bank, | Nakashima, Kenichi |

 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.　(Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB19575	pUlrTK	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pUlrTK was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19575).

Reference section:

Nakade, K., Tsukamoto, S., Nakashima, K., An, Y., Sato, I., Li, J., Shimoda, Y., Hemmi, Y., Miwa, Y., Hayashi, Y., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19575_B2Ddp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ColE1ori_F (pr 393) Region: ori, mini CMV pro.1,HSV TK.1 5' Sequence file: RDB19575_B2Dda.seq
>D08799_A9_1_B2Dd_ColE1ori_F_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNGNNNN NNNNNNNGNN NTGNNCNTCG ATTTTTGTGA TGCTCGTCAG    61 GGGGGCGGAG CCTATGGAAA AACGCCAGCA TATGAATTCA GTAGGCGTGT ACGGTGGGAG   121 GTCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC   181 TGTTTTGACC TCCATAGAAG ACACCGGGAC CGATCCAGCC TCCGCGGCCC CGAATTCCGG   241 GGATCGATCT TGGTGGCGTG AAACTCCCGC ACCTCTTCGG CAAGCGCCTT GTAGAAGCGC   301 GTATGGCTTC GTACCCCTGC CATCAACACG CGTCTGCGTT CGACCAGGCT GCGCGTTCTC   361 GCGGCCATAG CAACCGACGT ACGGCGTTGC GCCCTCGCCG GCAGCAAGAA GCCACGGAAG   421 TCCGCCTGGA GCAGAAAATG CCCACGCTAC TGCGGGTTTA TATAGACGGT CCTCACGGGA   481 TGGGGAAAAC CACCACCACG CAACTGCTGG TGGCCCTGGG TTCGCGCGAC GATATCGTCT   541 ACGTACCCGA GCCGATGACT TACTGGCAGG TGCTGGGGGC TTCCGAGACA ATCGCGAACA   601 TCTACACCAC ACAACACCGC CTCGACCAGG GTGAGATATC GGCCGGGGAC GCGGCGGTGG   661 TAATGACAAG CGCCCAGATA ACAATGGGCA TGCCTTATGC CGTGACCGAC GCCGTTCTGG   721 CTCCTCATAT CGGGGGGGAG GCTGGGAGCT CACATGCCCC GCCCCCGGCC CTCACCCTCA   781 TCTTCGANCG CCNTCCCATN NNCGCCCTCC TGTGCTACCC GGCCGCGCGA TACCTTATGG   841 GCAGCATGAN CCCCCANGCC GTGCTGGCGN TCGTGGNCCT CATCCCGNNN ANNTTGCCCN   901 GNACANNNTN GTGTTGGGGG NCCTTNNNGN NNNNANANNC ATCNACCGCC TGGCCAAACG   961 CCAGCNCCCC NGNNNANCNG NTTGACNNGG NNNNNCNGGN NNCNATTCNN NNNNTTTANN 1021 NNCNNNNTNN NNNTNNNGNN NNNNATNNNN NNNGNNNNNG NNNNNNNNNN //
Primer: phRLR1 (pr 219) Region: AmpR pro,HSV TK pA.2,HSV TK.2 3' Sequence file: RDB19575_B2Ddb.seq
>D08799_A9_1_B2Dd_phRLR1_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN TTTGATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG    61 CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC   121 CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA   181 AAACCTCTGA CACATGCAGC TCCCGGCTAT AGGAACAAAC GACCCAACAC CCGTGCGTTT   241 TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG   301 TTTCAGTTAG CCTCCCCCAT CTCCCGGGCA AACGTGCGCG CCAGGTCGCA GATCGTCGGT   361 ATGGAGCCGG GGGTGGTGAC GTGGGTCTGG ACCATCCCGG AGGTAAGTTG CAGCAGGGCG   421 TCCCGGCAGC CGGCGGGCGA TTGGTCGTAA TCCAGGATAA AGACGTGCAT GGGACGGAGG   481 CGTTTGGCCA AGACGTCCAA GGCCCAGGCA AACACGTTGT ACAGGTCGCC GTTGGGGGCC   541 AGCAACTCGG GGGCCCGAAA CAGGGTAAAT AACGTGTCCC CGATATGGGG TCGTGGGCCC   601 GCGTTGCTCT GGGGCTCGGC ACCCTGGGGC GGCACGGCCG TCCCCGAAAG CTGTCCCCAA   661 TCCTCCCGCC ACGACCCGCC GCCCTGCAGA TACCGCACCG TATTGGCAAG CAGCCCGTAA   721 ACGCGGCGAA TCGCGGCCAG CATAGCCAGG TCAAGCCGCT CGCCGGGGCG CTGGCGTTTG   781 GNCAGGCGGT CGATGTGTCT GTCNTCCGGA AGGGNCNNCA ACACGATGTT TGTGCCGGGC   841 AANGTCGGCG GGATGAGGGC CACGAACGCC AGCACGGCCT GGGGGGTCAT GCTGCCCATA   901 AGGNATCGCG CGGNCNNGNN AGCACAGGAN GGNGGNNATG GGNNGGCGGT CNAANANNAN   961 GGTGANGGNC GGGGGCGGGG NATGTNANCT CCCANNNNCC CCCCNNATNT GAGGNNNNNN 1021 NNNN //
Primer: M13fwd (pr 349) Region: HSV TK pA.1,HSV TK.1 3' Sequence file: RDB19575_B2Ddc.seq
>D08799_A9_1_B2Dd_M13fwd_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNAC CNNANNCCCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC    61 ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCATCTC   121 CCTGGCAAAC GTGCGCGCCA GGTCGCAGAT CGTCGGTATG GAGCCGGGGG TGGTGACGTG   181 GGTCTGGACC ATCCCGGAGG TAAGTTGCAG CAGGGCGTCC CGGCAGCCGG CGGGCGATTG   241 GTCGTAATCC AGGATAAAGA CGTGCATGGG ACGGAGGCGT TTGGCCAAGA CGTCCAAGGC   301 CCAGGCAAAC ACGTTGTACA GGTCGCCGTT GGGGGCCAGC AACTCGGGGG CCCGAAACAG   361 GGTAAATAAC GTGTCCCCGA TATGGGGTCG TGGGCCCGCG TTGCTCTGGG GCTCGGCACC   421 CTGGGGCGGC ACGGCCGTCC CCGAAAGCTG TCCCCAATCC TCCCGCCACG ACCCGCCGCC   481 CTGCAGATAC CGCACCGTAT TGGCAAGCAG CCCGTAAACG CGGCGAATCG CGGCCAGCAT   541 AGCCAGGTCA AGCCGCTCGC CGGGGCGCTG GCGTTTGGCC AGGCGGTCGA TGTGTCTGTC   601 CTCCGGAAGG GCCCCCAACA CGATGTTTGT GCCGGGCAAG GTCGGCGGGA TGAGGGCCAC   661 GAACGCCAGC ACGGCCTGGG GGGTCATGCT GCCCATAAGG TATCGCGCGG CCGGGTAGCA   721 CAGGAGGGCG GCGATGGGAT GGCGGTCGAA GATGAGGGTG AGGGCCGGGG GCGGGNNNTG   781 TGAGCTCCCA GCCTCCCCCC CGATATGAGG AGCCAGAACG GCGTCNGTCA CGGCATAAGG   841 CATGCCCATT GTTATCTGGG CGCTTGTCAT TACCACCGCC GCGTCCCCGG CCGATATCTC   901 ACCCTGGTCG ANGCGGTGTT GTGTGGNGTA GANGTTCGCG NNNGTCTCNG NANNCCCCCA   961 NCACCTGNCA GTAAGTCATC NGNNCGGGNA NGTAGNNNAN ATCGNNNCNC NNNCCCNNGG 1021 NNNNCNNCAN NNNNNNNGNN NGNNNNNTNN NNNNNNNCNN NNNGNNNNNN NNNN //
Primer: M13Fwd_R (pr 581) Region: mini CMV pro.2,HSV TK.2 5' Sequence file: RDB19575_B2Ddd.seq
>D08799_A9_1_B2Dd_M13Fwd_R_E01_13_ABI24.ab1     1 NNNNNNNNNG NNNNNGGNGG GNNGNCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA    61 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA   121 GCCTCCGCGG CCCCGAATTC CGGGGATCGA TCTTGGTGGC GTGAAACTCC CGCACCTCTT   181 CGGCAAGCGC CTTGTAGAAG CGCGTATGGC TTCGTACCCC TGCCATCAAC ACGCGTCTGC   241 GTTCGACCAG GCTGCGCGTT CTCGCGGCCA TAGCAACCGA CGTACGGCGT TGCGCCCTCG   301 CCGGCAGCAA GAAGCCACGG AAGTCCGCCT GGAGCAGAAA ATGCCCACGC TACTGCGGGT   361 TTATATAGAC GGTCCTCACG GGATGGGGAA AACCACCACC ACGCAACTGC TGGTGGCCCT   421 GGGTTCGCGC GACGATATCG TCTACGTACC CGAGCCGATG ACTTACTGGC AGGTGCTGGG   481 GGCTTCCGAG ACAATCGCGA ACATCTACAC CACACAACAC CGCCTCGACC AGGGTGAGAT   541 ATCGGCCGGG GACGCGGCGG TGGTAATGAC AAGCGCCCAG ATAACAATGG GCATGCCTTA   601 TGCCGTGACC GACGCCGTTC TGGCTCCTCA TATCGGGGGG GAGGCTGGGA GCTCACATGC   661 CCCGCCCCCG GCCCTCACCC TCATCTTCGA CCGCCATCCC ATCGCCGCCC TCCTGTGCTA   721 CCCGGCCGCG CGATACCTTA TGGGCAGCAT GACCCCCCAG GCCGTGCTGG CGTTCGTGGC   781 CCTCATCCCG CCGACCTTGC CCGGCACAAA CATCGTGTTG GGGGNCCTTC CGGANGACAG   841 ACACATCGNC CGCCTGGCCA AACGCCAGCG CCCCGGCGAG CGGCTTGACC TGGCTATGCT   901 GGNCGCGATT CGCCGCGTTT ANNGNNTGCT TGNCNNTNCN GNGCGGNNTC TGCAGGGCGG 961 NGGGTCNNNG NGGGANNNNT GGGNNNAGCT TTNNGGGNNN NNNNNNN //
Primer: rTKOutRev (pr 1236) Region: HSV TK pA.2,HSV TK.2 3' Sequence file: RDB19575_B2Dde.seq
>D08799A9_B2Dd_1_rTKOut_Rev_H08_23_ABI24     1 NNNNNNNNNN NNNNNNTTNN NTCTGTCTTT TNNTTGCCGT CATAGCGCGG GTTCCTTCCG    61 GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCATCT CCCGGGCAAA CGTGCGCGCC   121 AGGTCGCAGA TCGTCGGTAT GGAGCCGGGG GTGGTGACGT GGGTCTGGAC CATCCCGGAG   181 GTAAGTTGCA GCAGGGCGTC CCGGCAGCCG GCGGGCGATT GGTCGTAATC CAGGATAAAG   241 ACGTGCATGG GACGGAGGCG TTTGGCCAAG ACGTCCAAGG CCCAGGCAAA CACGTTGTAC   301 AGGTCGCCGT TGGGGGCCAG CAACTCGGGG GCCCGAAACA GGGTAAATAA CGTGTCCCCG   361 ATATGGGGTC GTGGGCCCGC GTTGCTCTGG GGCTCGGCAC CCTGGGGCGG CACGGCCGTC   421 CCCGAAAGCT GTCCCCAATC CTCCCGCCAC GACCCGCCGC CCTGCAGATA CCGCACCGTA   481 TTGGCAAGCA GCCCGTAAAC GCGGCGAATC GCGGCCAGCA TAGCCAGGTC AAGCCGCTCG   541 CCGGGGCGCT GGCGTTTGGC CAGGCGGTCG ATGTGTCTGT CCTCCGGAAG GGCCCCCAAC   601 ACGATGTTTG TGCCGGGCAA GGTCGGCGGG ATGAGGGCCA CGAACGCCAG CACGGCCTGG   661 GGGGTCATGC TGCCCATAAG GTATCGCGCG GCCGGGTAGC ACAGGAGGGC GGCGATGGGA   721 TGGCGGTCGA AGATGAGGGT GAGGGCCGGG GGCGGGGCAT GTGAGCTCCC AGCCTCCCCC   781 CCGATATGAG GAGCCAGAAC GGCGTCNGTC ACGGCATAAN GCATGCCCAT TGTTATCTGG   841 GCGCTTGTCA TTACCACCGC CNCGTCCCCN GCCGANATCT CACCCTGNTC GANGCGGTGT   901 TGTGTGGTGT AGANGTTCGC GANTGTCTCG NANNNCCCCA GCNNNTGCNN NNAANTCNTC   961 GGNTCNGGTA CNTANANNAN NTCNNCNNNC NNANCNNNNG GNCNNCANNA GTTGNNNGNN 1021 GGNNNGNTTT NCCCNTNCCN NNNNGNNCNN TNNNTNTAAN CNNNNNNNNN N //
Primer: AmpR_F (pr 962) Region: AmpR,5xUAS,ori Sequence file: RDB19575_B2Ddf.seq
>D08799_A9_1_B2Dd _AmpR_F_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNGNNN GNNCGCTGAG NNAGGTGCCT CACTGATTAA GCATTGGTAA    61 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT   121 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG   181 TTTTCGTTCC ACTGAGCGTC ACTGCAGGTC GATCGACGGA TCGGAGTACT GTCCTCCGAG   241 CGGAGTACTG TCCTCGAGCG GAGTACTGTC CTCCGAGCGG AGTACTGTCC TCCGAGCGGA   301 GTACTGTCCT CCGAGGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT   361 CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG   421 CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA   481 CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA   541 CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG   601 TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC   661 TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA   721 TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG   781 TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC   841 GCCTGGTATC TTTATAGTCC TGTCGGGNTT CGCCNCCTCT GACTTGAGCG TCGATTTTTN   901 TGANGCTCGT CANGGGGGNG GANCCTATNG AAAAACGCCN GCATATGAAN TCNGTNNNNG   961 TGNACNNNGG GAGGNCTATA TNAGCAGANC TCGTTTANTG NNNNNNCNNN TNNNNNNNNN 1021 N //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

original	Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]

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