Mammalian expression and T7 In Vitro Transcription dual vector of SARS-CoV-2 ORF1ab. Bacterial low copy number plasmid.
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| Clone info. | mammalian expression and T7 In Vitro Transcription dual vector of SARS-CoV-2 ORF1ab. Bacterial low copy number plasmid. |
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| Comment | delivative of pMWT7-KpnI-polyA (RDB19684). |
| Vector backbone | pMWT7-KpnI-pA (RDB19684) (plasmid) |
| Growth conditions | LB+kan, 23dC |
| Growth remarks | Recommended host, DH10B; Slow growth, extremely low yield; Bacterial genome insertion mutation may occur. |
| Gene/insert name | SARS-CoV-2 ORF1ab genomic DNA. |
| Depositor|Developer | DNA Bank, | Nakashima, Kenichi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment MTA, for use for not-for-profit academic purpose [Word Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. () |
[open/close]| 必要書類 | 提供依頼書 提供同意書 (MTA, 非営利学術目的用)[Word 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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| MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する()。 |
| Catalog # | Resource name | Shipping form | Fee |
|---|---|---|---|
| RDB19687 | pMWT7-CoV-ORF1ab-pA | DNA solution |
Materials & Methods section:
| The pMWT7-CoV-ORF1ab-pA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19687). |
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