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pUlrTK H2BC21-G-EZ (#RDB19577)

Genome editing tool. Donner vector to tag EGFP at human histon H2BC21, C-terminaly. Selection with Zeo & GCV


Drawn by SnapGene® software
Sequence (full) RDB19577hts01.seq
Publication Nakade, K., Cell Rep. Methods 3 (12): 100662 (2023). [link to RRC of NBRP]
Test sheet RDB19577_B2Ddp1-1.pdf 
Assembled from experimentally sequenced data.
 
Alternative name pUC2x2Tk-H2B-GEZ
Clone info. Genome editing tool. Donner vector to tag EGFP at human histon H2BC21, C-terminaly. Selection with Zeo & GCV
Vector backbone pwTK5UAS (RDB19575) (plasmid)
Size of vector backbone 4.9 kb
Selectable markers Ampicillin
Gene/insert name human H2BC21 left and right arms genomic DNA; Aequorea victoria GFP (EGFP) cDNA.
Depositor|Developer DNA Bank, | Nakashima, Kenichi |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.　(Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))。

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RDB19577 pUlrTK H2BC21-G-EZ DNA solution

How to cite this biological resource

Materials & Methods section:

The pUlrTK H2BC21-G-EZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19577).

Reference section:

Nakade, K., Tsukamoto, S., Nakashima, K., An, Y., Sato, I., Li, J., Shimoda, Y., Hemmi, Y., Miwa, Y., Hayashi, Y., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]