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pX330_CDM24-1 (#RDB19580)

Genome editing tool. sgRNA and SpCas9 expression in one vector. Subcloned an oligo nucleotide to target human POU5F1 gene stop codon nearby.


Drawn by SnapGene® software
Sequence (full) RDB19580hts01.seq
Publication Nakade, K., Cell Rep. Methods 3 (12): 100662 (2023). [link to RRC of NBRP]
Test sheet RDB19580_B2Ddp1-1.pdf 
Assembled from experimentally sequenced data.
 
Alternative name pX330-gOCT3/4
Clone info. Genome editing tool. sgRNA and SpCas9 expression in one vector. Subcloned 5'-gaacttaatcccaaaaaccc-3' in between BbsI sites to target human POU5F1 gene stop codon nearby.
Vector backbone pX330 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Ampicillin
Gene/insert name synthetic human POU5F1 gene targeting gRNA.
Depositor|Developer DNA Bank, | Nakashima, Kenichi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.　(Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))
Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19580 pX330_CDM24-1 DNA solution

How to cite this biological resource

Materials & Methods section:

The pX330_CDM24-1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19580).

Reference section:

Nakade, K., Tsukamoto, S., Nakashima, K., An, Y., Sato, I., Li, J., Shimoda, Y., Hemmi, Y., Miwa, Y., Hayashi, Y., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]