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pUCtk2x2_MYH7-TEZ

Genome editing tool. Donner vector to tag 2A-tdTomato at human MYH7, C-terminaly. Selection with Zeo & GCV.

Catalog number RDB20120
Resource name pUCtk2x2_MYH7-TEZ
Clone info. Genome editing tool. Donner vector to tag 2A-tdTomato at human MYH7, C-terminaly. Selection with Zeo & GCV.
HSV tk at left arm side contains frameshift mutation.
Vector backbone pUCtk2x2 (plasmid)
Gene/insert name Herpes simplex virus (HSV) thymidin kinase (TK) gene; human MYH7 genomic DNA; Dscosoma sp. red fluorescent protein (tdTomato); human EEF1A1 promoter; Streptoalloteichus hindustanus bleomycin resistant gene.
Depositor|Developer DNA Bank, | Nakashima, Kenichi |
 
Sequence (full) RDB20120hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB20120_B3Jvp1-1.pdf 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.　(Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))。

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RDB20120 pUCtk2x2_MYH7-TEZ DNA solution

How to cite this biological resource

Materials & Methods section:

The pUCtk2x2_MYH7-TEZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20120).

Reference section:

Nakade, K., Tsukamoto, S., Nakashima, K., An, Y., Sato, I., Li, J., Shimoda, Y., Hemmi, Y., Miwa, Y., Hayashi, Y., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]