Yoichi Shinkai's Resource

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Yoichi Shinkai's Resource

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Catalog #	Name of clone	Running title	Publication
RDB16921	pPB-CAG-3xFLAG-MORC2A_DD68/69AA-IRES-puro	Expression vector of mouse MORC2A (DD68/69AA) to generate stably expressing ES cells.	Fukuda, K., A CRISPR knockout screen identifies SETDB1-target retroelement silencing factors in embryonic stem cells. Genome Res. 28 (6): 846-858 (2018). PMID 29728365. [PubMed] [Article] [RRC of NBRP] (original)
RDB16920	pPB-CAG-V5-RESF1-IRES-blast	Expression vector of mouse RESF1 (WT) to generate stably expressing ES cells.	Fukuda, K., A CRISPR knockout screen identifies SETDB1-target retroelement silencing factors in embryonic stem cells. Genome Res. 28 (6): 846-858 (2018). PMID 29728365. [PubMed] [Article] [RRC of NBRP] (original)
RDB16919	pPB-CAG-3xFLAG-RESF1-IRES-puro	Expression vector of mouse RESF1 (WT) to generate stably expressing ES cells.	Fukuda, K., A CRISPR knockout screen identifies SETDB1-target retroelement silencing factors in embryonic stem cells. Genome Res. 28 (6): 846-858 (2018). PMID 29728365. [PubMed] [Article] [RRC of NBRP] (original)
RDB16918	pPB-CAG-V5-MORC2A-IRES-blast	Expression vector of mouse MORC2A (WT) to generate stably expressing ES cells.	Fukuda, K., A CRISPR knockout screen identifies SETDB1-target retroelement silencing factors in embryonic stem cells. Genome Res. 28 (6): 846-858 (2018). PMID 29728365. [PubMed] [Article] [RRC of NBRP] (original)
RDB16917	pPB-CAG-3xFLAG-MORC2A_S737A-IRES-puro	Expression vector of mouse MORC2A (S737A) to generate stably expressing ES cells.	Fukuda, K., A CRISPR knockout screen identifies SETDB1-target retroelement silencing factors in embryonic stem cells. Genome Res. 28 (6): 846-858 (2018). PMID 29728365. [PubMed] [Article] [RRC of NBRP] (original)
RDB16916	pPB-CAG-3xFLAG-MORC2A_deltaChromoLike-IRES-puro	Expression vector of mouse MORC2A (deltaCL_delta793-852) to generate stably expressing ES cells.	Fukuda, K., A CRISPR knockout screen identifies SETDB1-target retroelement silencing factors in embryonic stem cells. Genome Res. 28 (6): 846-858 (2018). PMID 29728365. [PubMed] [Article] [RRC of NBRP] (original)
RDB16915	pPy-CAG-3xFLAG-MORC2A_deltaCW-IRES-puro	Expression vector of mouse MORC2A (deltaCW_delta495-542) to generate stably expressing ES cells.	Fukuda, K., A CRISPR knockout screen identifies SETDB1-target retroelement silencing factors in embryonic stem cells. Genome Res. 28 (6): 846-858 (2018). PMID 29728365. [PubMed] [Article] [RRC of NBRP] (original)
RDB16914	pPy-CAG-3xFLAG-MORC2A_deltaATPase-IRES-puro	Expression vector of mouse MORC2A (deltaATPase_delta24-134) to generate stably expressing ES cells.	Fukuda, K., A CRISPR knockout screen identifies SETDB1-target retroelement silencing factors in embryonic stem cells. Genome Res. 28 (6): 846-858 (2018). PMID 29728365. [PubMed] [Article] [RRC of NBRP] (original)
RDB16913	pPy-CAG-3xFLAG-MORC2A-IRES-puro	Expression vector of mouse MORC2A (WT) to generate stably expressing ES cells.	Fukuda, K., A CRISPR knockout screen identifies SETDB1-target retroelement silencing factors in embryonic stem cells. Genome Res. 28 (6): 846-858 (2018). PMID 29728365. [PubMed] [Article] [RRC of NBRP] (original)
RDB15901	pMCs-GFP-Suv39h1-mut3	Retroviral expression vector of mouse SUV39H1, nucleic acid-binding defective mutant (R99A, R100A).	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15900	pMCs-GFP-Suv39h1-mut1	Retroviral expression vector of mouse SUV39H1, nucleic acid-binding defective mutant (R84A, K87A).	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15899	pMCs-GFP-Suv39h1-Y67A-4A	Retroviral expression vector of mouse SUV39H1, nucleic acid-binding defective mutant and methylated H3K9 binding mutant (R84A, K87A, K91A, R95A and Y67A).	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15898	pMCs-GFP-Suv39h1-Y67A	Retroviral expression vector of mouse SUV39H1, methylated H3K9 binding mutant (Y67A).	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15897	pMCs-GFP-Suv39h1-4A	Retroviral expression vector of mouse SUV39H1, nucleic acid-binding defective mutant (R84A, K87A, K91A, R95A).	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15896	pMCs-GFP-Suv39h1	Retroviral expression vector of mouse SUV39H1, wild type.	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15895	pCAG-FLAG-IRESpuro (NRI)-mmSuv39H1-mut3	Expression vector of mouse SUV39H1, nucleic acid-binding defective mutant (R99A, R100A).	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15894	pCAG-FLAG-IRESpuro (NRI)-mmSuv39H1-mut1	Expression vector of mouse SUV39H1, nucleic acid-binding defective mutant (R84A, K87A).	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15893	pCAG-FLAG-IRESpuro (NRI)-mmSuv39H1-Y67A-4A	Expression vector of mouse SUV39H1, nucleic acid-binding defective mutant and methylated H3K9 binding mutant (R84A, K87A, K91A, R95A and Y67A).	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15892	pCAG-FLAG-IRESpuro (NRI)-mmSuv39H1-Y67A	Expression vector of mouse SUV39H1, methylated H3K9 binding mutant (Y67A).	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15891	pCAG-FLAG-IRESpuro (NRI)-mmSuv39H1-4A	Expression vector of mouse SUV39H1, nucleic acid-binding defective mutant (R84A, K87A, K91A, R95A).	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15890	pCAG-FLAG-IRESpuro (NRI)-mmSuv39H1-H324L	Expression vector of mouse SUV39H1, SET domain mutant (H324L).	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15889	pCAG-FLAG-IRESpuro (NRI)-mmSuv39H1	Expression vector of mouse SUV39H1, wild type.	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15888	pMCs-FLAG-Suv39h1-R24,K27A-IRES-GFP	Retroviral expression vector of mouse SUV39H1, N-terminal mutant (R24A, K27A).	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15887	pMCs-FLAG-Suv39h1-deltaN-IRES-GFP	Retroviral expression vector of mouse SUV39H1, N-terminal truncated mutant (delta 1-41).	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15886	pMCs-FLAG-Suv39h1-Y67A-4A-IRES-GFP	Retroviral expression vector of mouse SUV39H1, nucleic acid-binding defective mutant and methylated H3K9 binding mutant (R84A, K87A, K91A, R95A and Y67A).	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15885	pMCs-FLAG-Suv39h1-Y67A-IRES-GFP	Retroviral expression vector of mouse SUV39H1, methylated H3K9 binding mutant (Y67A).	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15884	pMCs-FLAG-Suv39h1-4A-IRES-GFP	Retroviral expression vector of mouse SUV39H1, nucleic acid-binding defective mutant (R84A, K87A, K91A, R95A).	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15883	pMCs-FLAG-Suv39h1-IRES-GFP	Retroviral expression vector of mouse SUV39H1, wild type.	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15882	pGEX6P-3-SUV39H1(39-105)-Y67A	Expression vector of mouse SUV39H1, chromodomain (residues 39-105), methylated H3K9 binding mutant (Y67A).	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15881	pGEX6P-3-SUV39H1(39-105)-4A	Expression vector of mouse SUV39H1, chromodomain (residues 39-105), nucleic acid-binding defective mutant (R84A, K87A, K91A, R95A).	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB15880	pGEX6P-3-SUV39H1(39-105)	Expression vector of mouse SUV39H1, chromodomain (residues 39-105).	Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [PubMed] [Article] [RRC of NBRP] (original)
RDB05729	14-1	Plasmid clone of mouse histone methyltransferase, GLP	Tachibana, M., Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9. Genes Dev., 19, 815-826 (2005). PMID 15774718. [PubMed] [Article] [RRC of NBRP] (original)
RDB05728	pZErO-2.1 mG9a-L	Plasmid clone of mouse histone methyltransferase, G9a	Tachibana, M., G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis. Genes Dev., 16, 1779-1791 (2002). PMID 12130538. [PubMed] [Article] [RRC of NBRP] (original)
RDB05727	pZErO-2.1 mG9a-S	Plasmid clone of mouse histone methyltransferase, G9a	Tachibana, M., G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis. Genes Dev., 16, 1779-1791 (2002). PMID 12130538. [PubMed] [Article] [RRC of NBRP] (original)
RDB01181	YT101	Human perforin cDNA	Shinkai, Y., Molecular cloning and chromosomal assignment of a human perforin (PFP) gene. Immunogenetics, 30, 452-457 (1989). PMID 2592021. [PubMed] [Article] [RRC of NBRP] (original)

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2026.03.23

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