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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pZErO-2.1 mG9a-L (#RDB05728)

Plasmid clone of mouse histone methyltransferase, G9a

Clone info. Plasmid clone of mouse histone methyltransferase, G9a.
Vector backbone pZErO-2.1 (Plasmid)
Size of vector backbone 3.3 kb
Selectable markers Kan^r
Gene/insert name Mouse G9a cDNA
Reference of insert sequence AB077210 (DDBJ accession number)
Depositor|Developer Shinkai, Yoichi | Tachibana, Makoto |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Genes Dev., 16, 1779-1791, 2002).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Genes Dev., 16, 1779-1791, 2002)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05728 pZErO-2.1 mG9a-L DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pZErO-2.1 mG9a-L was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05728).

Reference section:

Tachibana, M., Sugimoto, K., Nozaki, M., Ueda, J., Ohta, T., Ohki, M., Fukuda, M., Takeda, N., Niida, H., Kato, H., Shinkai, Y., G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis. Genes Dev., 16, 1779-1791 (2002). PMID 12130538. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12373_A4Atp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB12373_A4Ata.seq check
>05728_12373_A4At_M13(-40)_C12_09.ab1
    1 CAACGGTATG TAATACGACT TCGAATAGGG CGAATTGGGC CCTCTAGATG CATGCTCGAG
   61 CGGCCGCCAG TGTGATGGAT ATCTGCAGAA TTCGCGGCCG CGTCGACCGG CCCCAGAGAT
  121 GCGGGGTCTG CCGAGAGGGA GGGGGCTGAT GCGGGCCCGG GGGCGGGGGC GTGCGGCCCC
  181 CACGGGCGGC CGCGGCCGCG GTCGGGGGGG CGCCCACCGA GGGCGAGGTA GGCCCCGAAG
  241 CCTGCTCTCG CTGCCCAGGG CCCAGGCGTC TTGGGCCCCC CAGCTGCCTG CCGGGCTGAC
  301 CGGCCCCCCG GTTCCTTGTC TCCCCTCCCA GGGGGAGGCC CCCGCTGAGA TGGGGGCGCT
  361 GCTGCTGGAG AAGGAGCCCC GAGGAGCCGC CGAGAGAGTT CATAGCTCTT TGGGGGACAC
  421 CCCTCAGAGT GAGGAGACCC TTCCCAAGGC CAACCCCGAC TCCTTGGAGC CTGCCGGCCC
  481 CTCCTCTCCG GCCTCTGTCA CTGTCACCGT CGGCGATGAG GGGGCTGACA CCCCTGTCGG
  541 GGCCGCATCA CTCATCGGGG ACGAACCCGA GAGCCTGGAG GGAGATGGGG GTCGCATCGT
  601 GCTGGGCCAT GCCACAAAGT CGTTCCCCTC TTCCCCCAGC AAGGGGGGTG CCTGTCCCAG
  661 TCGGGCCAAA ATGTCAATGA CAGGGGCAGG AAAGTCGCCC CCCTCGGTCC AGAGTTTGGC
  721 CATGAGGCTG TTGAGCATGC CCGGGGCCCA GGGAGCTGCA ACTGCTGGGC CTGAACCCTC
  781 TCCGGCAACA ACTGCCGCCC AGGAGGGGCA GCCCAAAGTG CACCGAGCCC GGAAAACCAT
  841 GTCCAAACCT AGCAACGGAC AGCCTCCAAT CCCTGAGAAG CGGCCCCCTG AAGTCCAGCA
  901 TTTCCGCATG AGTGATGACA TGCATCTGGG GAAGGTGACT TCAGATGTGG CCAAAAGGAG
  961 GAAGCTGAAC TCTGGTAGCC TGTCCGAGGA CTTGGGCTCT GCCGGGGGGC TCAGGAGATA
 1021 TAATCCCTGG AGAAGGAGAG CCCAGGCCCC TGGAGAGTGG AGACGGTGGT GGGCGATGAC
 1081 TTCAGCTGTA CTATGATGCG TACCTCTGTG CATGACCGGA TTGAACTCTG ACAGGCCAGT
1141 CTGCAGTCGG AAGCTCTAGC TTGAACCAAG TGA
//
Primer: Reverse2
Sequence file: RDB12373_A4Atb.seq check
>05728_12373_A4At_Reverse2_D12_12.ab1
    1 CTTTTAGGTG ACTATAGAAT ACTCAAGCTA TGCATCAAGC TTGGTACCGA GCTCGGATCC
   61 ACTAGTAACG GCCGCCAGTG TGCTGGAATT CGCGGCCGCG TCGACTTTTT TTTTACCAAA
  121 CCCAACATTT ATTGAGAACA CAGAAAATCA GCTGGCAGAG GCCCAACTTC AATCAGCTAA
  181 CTGAGGCCAG GCTCAGTGGG GAAGGCCAGC CCTGAAGTCA CTTGCACTCC CAGTGAGAAA
  241 CCAGCTCTGG GAGAGGGGCC ATCGACCTTC CAGGCCTTAC TGGGGTCTCT GATGGAGAAA
  301 TGTCTGAAGG GCAGTGCCCG GCCTGGATTT TAAGAGTCCT CAGGTGTTGA TGGGGGGCAG
  361 GGAGCTGAGG TCAGGGAGCA GCTCCGGGTG GGGGTCCAGC CGGGCCAGGC GGCTCTGCTC
  421 CAGGGCGATG GCCTCCGCTG AATGCTTGCA CTTCTCAGAG CCACACTGGC AGGTGAAATA
  481 CTTGCTCTTG ATGTCCCAGA ATCGGTCACC GTAGTCAAAG CCCAGCTCCT CCCCAGTCCG
  541 GATGTCCCTG GAGCTGAAGA AGGCAATGCG TGGGAACCGT AGATCTTGGT GCAGCATGAA
  601 AACCCGGACA GGGATGATGT TGGGGTCACA CAGGTGGTTA ATGAATCGGC TGATGTTGCC
  661 ATAGTAACGG GCATCAATGC AGTAAACCTC GCCATCCTTG TTATCTAAAT CGAAGAGGTA
  721 AGAATCATCC TCTCTCACAT CAGCCTCGGC ATCAGAGATC AGCTCTCCTA CATACTCGCA
  781 GATGAACGTG CCCTGGGGGA TGGTCTGCAA GGCTCGGACC CCCCAGCCCA TCTTGGCAGT
  841 CCGGTAGAGC TGCAGCCGTA CCTTGATGCC GCTCTGCACC ACGCGGTTCT TGCAGCTTCT
  901 CCAGCAGGAG CATGCCTGGT TACACTCAAA GATCAGGGGG GCTCGATCTT GTTAAACTCC
  961 TGGAGCAGCC GCCCGTCCTT GTCATACCAG CATCGGATAC TGAGCTGACC ACATAGGCAA
 1021 TTGGAGCTGG AGCAGTCATC CACACACGTG CAGTGCTGCA GATGGGTGAT GTTGCGTCGA
 1081 TGTCATGATC GATGTCTCGC AGGTCCTCAG AGATGTACTT GTAGTCCTCC GGCACGGCTC
 1141 CAATCCACAT CATGGAACCA GGATGTACAG TTTCTCTATA GCTTCGGCCT ACGGTTCCGC
1201 A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tachibana, M., G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis. Genes Dev., 16, 1779-1791 (2002). PMID 12130538. [link to RRC of NBRP]