Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pZErO-2.1 mG9a-S (#RDB05727)

Plasmid clone of mouse histone methyltransferase, G9a

Clone info. Plasmid clone of mouse histone methyltransferase, G9a.
Vector backbone pZErO-2.1 (Plasmid)
Size of vector backbone 3.3 kb
Selectable markers Kan^r
Gene/insert name Mouse G9a cDNA
Reference of insert sequence AB077209 (DDBJ accession number)
Depositor|Developer Shinkai, Yoichi | Tachibana, Makoto |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Genes Dev., 16, 1779-1791, 2002).
提供案内 (日本国内) [open/close]

MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Genes Dev., 16, 1779-1791, 2002)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05727 pZErO-2.1 mG9a-S DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pZErO-2.1 mG9a-S was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05727).

Reference section:

Tachibana, M., Sugimoto, K., Nozaki, M., Ueda, J., Ohta, T., Ohki, M., Fukuda, M., Takeda, N., Niida, H., Kato, H., Shinkai, Y., G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis. Genes Dev., 16, 1779-1791 (2002). PMID 12130538. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15802_A8Bgp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB15802_A8Bga.seq check
>05727_15802_A8Bg_M13_-40_E02_14_ABI24.ab1
    1 AGGGGCGGGG TAAATGGGAA TACGGACTTC GAATAGGGCG AATTGGGCCC TCTAGATGCA
   61 TGCTCGAGCG GCCGCCAGTG TGATGGATAT CTGCAGAATT CGCGGCCGCG TCGACGCGAT
  121 GGCGGCGGCG GCGGGAGCTG CTGCGGCGGC GGCCGCCGAG GGGGAGGCCC CCGCTGAGAT
  181 GGGGGCGCTG CTGCTGGAGA AGGAGCCCCG AGGAGCCGCC GAGAGAGTTC ATAGCTCTTT
  241 GGGGGACACC CCTCAGAGTG AGGAGACCCT TCCCAAGGCC AACCCCGACT CCTTGGAGCC
  301 TGCCGGCCCC TCCTCTCCGG CCTCTGTCAC TGTCACCGTC GGCGATGAGG GGGCTGACAC
  361 CCCTGTCGGG GCCGCATCAC TCATCGGGGA CGAACCCGAG AGCCTGGAGG GAGATGGGGG
  421 TCGCATCGTG CTGGGCCATG CCACAAAGTC GTTCCCCTCT TCCCCCAGCA AGGGGGGTGC
  481 CTGTCCCAGT CGGGCCAAAA TGTCAATGAC AGGGGCAGGA AAGTCGCCCC CCTCGGTCCA
  541 GAGTTTGGCC ATGAGGCTGT TGAGCATGCC CGGGGCCCAG GGAGCTGCAA CTGCTGGGCC
  601 TGAACCCTCT CCGGCAACAA CTGCCGCCCA GGAGGGGCAG CCCAAAGTGC ACCGAGCCCG
  661 GAAAACCATG TCCAAACCTA GCAACGGACA GCCTCCAATC CCTGAGAAGC GGCCCCCTGA
  721 AGTCCAGCAT TTCCGCATGA GTGATGACAT GCATCTGGGG AAGGTGACTT CAGATGTGGC
  781 CAAAAGGAGG AAGCTGAACT CTGGTAGCCT GTCCGAGGAC TTGGGCTCTG CCGGGGGGCT
  841 CAGGAGATAT AATCCTGGAG AAGGGAGAGC CCAGGCCCCT GGAGGAGTGG GAGACGGTGG
  901 TGGGCGATGA CTTCAGCCTG TACTATGATG CGTACTCTGT GGATGAGCGG GTGGACTCTG
  961 ACAGCAAGTC TGAAGTCGAA GCTCTAGCTG AACAGTTGAG TGAGGAGGAG GAGGAGGAAG
1021 AGGAGGAAGA GAGGAAGAGG AAGGGAAGGA GGA
//
Primer: Reverse2
Sequence file: RDB15802_A8Bgb.seq check
>05727_15802_A8Bg_Reverse2_F02_17_ABI24.ab1
    1 ATTAAATGGA ATTACGGCCA GGCTATTTAG GTGACACTAT AGAATACTCA AGCTATGCAT
   61 CAAGCTTGGT ACCGAGCTCG GATCCACTAG TAACGGCCGC CAGTGTGCTG GAATTCGCGG
  121 CCGCGTCGAC TTTTTTTTTT TTTACCAAAC CCAACATTTA TTGAGAACAC AGAAAATCAG
  181 CTGGCAGAGG CCCAACTTCA ATCAGCTAAC TGAGGCCAGG CTCAGTGGGG AAGGCCAGCC
  241 CTGAAGTCAC TTGCACTCCC AGTGAGAAAC CAGCTCTGGG AGAGGGGCCA TCGACCTTCC
  301 AGGCCTTACT GGGGTCTCTG ATGGAGAAAT GTCTGAAGGG CAGTGCCCGG CCTGGATTTT
  361 AAGAGTCCTC AGGTGTTGAT GGGGGGCAGG GAGCTGAGGT CAGGGAGCAG CTCCGGGTGG
  421 GGGTCCAGCC GGGCCAGGCG GCTCTGCTCC AGGGCGATGG CCTCCGCTGA ATGCTTGCAC
  481 TTCTCAGAGC CACACTGGCA GGTGAAATAC TTGCTCTTGA TGTCCCAGAA TCGGTCACCG
  541 TAGTCAAAGC CCAGCTCCTC CCCAGTCCGG ATGTCCCTGG AGCTGAAGAA GGCAATGCGT
  601 GGGAACCGTA GATCTTGGTG CAGCATGAAA ACCCGGACAG GGATGATGTT GGGGTCACAC
  661 AGGTGGTTAA TGAATCGGCT GATGTTGCCA TAGTAACGGG CATCAATGCA GTAAACCTCG
  721 CCATCCTTGT TATCTAAATC GAAGAGGTAA GAATCATCCT CTCTCACATC AGCCTCGGCA
  781 TCAGAGATCA GCTCTCCTAC ATACTCGCAG ATGAACGTGC CCTGGGGGAT GGTCTGCAAG
  841 GCTCGGACCC CCCAGCCCAT CTTGGCAGTC CGGTAGAGCT GCAGCCGTAC CTTGATGCCG
  901 CTCTGCACCA CGCGGTTCTT GCAGCTTCTC CAGCAGGAGC ATGCCTGGGT TACACTCAAA
  961 GATCAGGGGG GGCTCGATCT TGTTTAACTC CTGGAGCAGC CCGCCCGTCC CTTGTCATAC
 1021 CAGCATCGGA TACTGAGCTG ACCACATAGG CATTGGGAGC TGGGAGCAGT CATCCACCAC
 1081 CACGGTGCAG TGCTTGCAGA TGGGGTTGAT TGTTTGCGTC GGAATTGTTC ATTGGTCGAA
1141 TTGTTCTCGC AGTTCTCAGA GAATGTACTG AGTCCTCAGG GCACCGTGCG AGCCT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tachibana, M., G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis. Genes Dev., 16, 1779-1791 (2002). PMID 12130538. [link to RRC of NBRP]