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pCAG-FLAG-IRESpuro (NRI)-mmSuv39H1

Expression vector of mouse SUV39H1, wild type.

Catalog number RDB15889
Resource name pCAG-FLAG-IRESpuro (NRI)-mmSuv39H1
Clone info. Expression vector of mouse suppressor of variegation 3-9 homolog 1 (SUV39H1), wild type, tagged with N-terminal FLAG, fused with C-terminal IRES.
Comment Expression was confirmed by the depositor with Western blot.
Vector backbone pCAG-FLAG-IRESpuro (NRI) (plasmid)
Size of vector backbone 6.5 kb
Selectable markers Amp^r
Gene/insert name mouse Suv39h1 cDNA
Depositor|Developer Shinkai, Yoichi |
Other clones in our bank
External Database
mouse Suv39h1
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Shirai, A et al. ELIFE. (6), e25317, 2017)
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shirai, A et al. ELIFE. (6), e25317, 2017)。
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB15889 pCAG-FLAG-IRESpuro (NRI)-mmSuv39H1 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201.

          2022.05.17

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