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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX6P-3-SUV39H1(39-105)-4A (#RDB15881)

Expression vector of mouse SUV39H1, chromodomain (residues 39-105), nucleic acid-binding defective mutant (R84A, K87A, K91A, R95A).

Clone info. Expression vector of mouse suppressor of variegation 3-9 homolog 1 (SUV39H1), chromodomain (CD, residues 39-105), nucleic acid-binding defective mutant (R84A, K87A, K91A, R95A) fused with N-terminal GST, tac promoter, see Fig. 1A and Fig. 2A of Shirai, A. et al., Elife, 1; 6. pii: e25317, 2017.
Comment Expression was confirmed by the depositor with CBB staining and Western blot.
Vector backbone pGEX-6P-3 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name mouse Suv39h1 cDNA
Depositor|Developer Shinkai, Yoichi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Shirai, A et al. ELIFE. (6), e25317, 2017)
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shirai, A et al. ELIFE. (6), e25317, 2017)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15881 pGEX6P-3-SUV39H1(39-105)-4A DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX6P-3-SUV39H1(39-105)-4A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15881).

Reference section:

Shirai, A., Kawaguchi, T., Shimojo, H., Muramatsu, D., Ishida-Yonetani, M., Nishimura, Y., Kimura, H., Nakayama, J.I., Shinkai, Y., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15881_A8Dgp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX_F2
Sequence file: RDB15881_A8Dga.seq check
>D05294A2-A8Dg__2_pGEX_F2_G02_07_ABI08.ab1
    1 GCATTTAGGT TTGGTTTTAA AAACGTATTG AAGCTATCCC ACAAAATTGA TAAGTACTTG
   61 AAATCCAGCA AGTATATAGC ATGGCCTTTG CAGGGCTGGC AAGCCACGTT TGGTGGTGGC
  121 GACCATCCTC CAAAATCGGA TCTGGAAGTT CTGTTCCAGG GGCCCCTGGG ATCCAATCTG
  181 TATGACTTTG AAGTTGAATA CCTGTGTGAT TATAAGAAGA TCCGTGAGCA GGAGTATTAC
  241 CTGGTTAAGT GGCGTGGGTA TCCCGACTCA GAAAACACCT GGGAGCCACG GCAGAATCTA
  301 AAATGTATAG CAGTTCTTGC ACAGTTCCAC GCAGACTTAG AAGCAGAGCT TGTCCGACGA
  361 CACCGCCGGT CAAAGTGAGT CGACTCGAGC GGCCGCATCG TGACTGACTG ACGATCTGCC
  421 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
  481 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
  541 TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG CGATAGCGGA GTGTATAATT
  601 CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA
  661 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT
  721 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC
  781 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC
  841 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA
  901 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG
  961 GTAAGATCCT TGAGAGTTTT CGCCCCGGAA GAACGTTTTC CCAATGATGA GCACTTTTAA
 1021 AGTTTCTGCT ATGTGGCGCG GGTATTAATC CCGTGTTTGA CGCCCGGGCA AGAGCAACTC
 1081 GGGTCGCCGC ATACACTATT TCTCAGAATG ACTGGTGGAA GTTACTTCAC CCAGTTCAAC
1141 AGAAAAAGGC AATTCCTTAA CCGGAATGTG GGC
//
Primer: pGEX-ptac_up-F
Sequence file: RDB15881_A8Dgb.seq check
>D05294A2-2_A8Dg_pGEX-ptac_up-F_C01_03_ABI08.ab1
    1 ATATTGGCGG GAATAAAAAC GGGTTCTGGG CAATATTCTG AAATGAGCTG TTGACAATTA
   61 ATCATCGGCT CGTATAATGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG
  121 TATTCATGTC CCCTATACTA GGTTATTGGA AAATTAAGGG CCTTGTGCAA CCCACTCGAC
  181 TTCTTTTGGA ATATCTTGAA GAAAAATATG AAGAGCATTT GTATGAGCGC GATGAAGGTG
  241 ATAAATGGCG AAACAAAAAG TTTGAATTGG GTTTGGAGTT TCCCAATCTT CCTTATTATA
  301 TTGATGGTGA TGTTAAATTA ACACAGTCTA TGGCCATCAT ACGTTATATA GCTGACAAGC
  361 ACAACATGTT GGGTGGTTGT CCAAAAGAGC GTGCAGAGAT TTCAATGCTT GAAGGAGCGG
  421 TTTTGGATAT TAGATACGGT GTTTCGAGAA TTGCATATAG TAAAGACTTT GAAACTCTCA
  481 AAGTTGATTT TCTTAGCAAG CTACCTGAAA TGCTGAAAAT GTTCGAAGAT CGTTTATGTC
  541 ATAAAACATA TTTAAATGGT GATCATGTAA CCCATCCTGA CTTCATGTTG TATGACGCTC
  601 TTGATGTTGT TTTATACATG GACCCAATGT GCCTGGATGC GTTCCCAAAA TTAGTTTGTT
  661 TTAAAAAACG TATTGAAGCT ATCCCACAAA TTGATAAGTA CTTGAAATCC AGCAAGTATA
  721 TAGCATGGCC TTTGCAGGGC TGGCAAGCCA CGTTTGGTGG TGGCGACCAT CCTCCAAAAT
  781 CGGATCTGGA AGTTCTGTTC CAGGGGCCCC TGGGATCCAA TCTGTATGAC TTTGAAGTTG
  841 AATACCTGTG TGATTATAAG AAGATCCGTG AGCAGGAGTA TTACCTGGTT TAAGTGGCGT
  901 GGGTATCCCG ACTCAGAAAA CACCTGGGAG CCACGGCAGA ATCTAAAATG TATAGCAGTT
  961 CTTTGCACAG TTCCACGCAG ACTTAAGAAG CAGAGCTTTG TTCCGACGAC ACCGCCCGGT
 1021 TCAAAGTGAG TCGACTCGAG CGGCCCGGCA TCGGTGAACC TGGACTGACC GATCTGCCTC
1081 GCGCGATTCC GGTTGAATGA ACCGGCTGGA AAAACCCTTC TTGAACCACA ATTGCCAA
//
Primer: SV40pA3' element Reverse
Sequence file: RDB15881_A8Dgc.seq check
>D05294A2-2_A8Dg_SV40pA3'elementReverse_D01_04_ABI08.ab1
    1 GAGTCTTTGG GGGGGCGGGG GGCTATGGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT
   61 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG
  121 TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG
  181 AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA
  241 CGCATCTGTG CGGTATTTCA CACCGCATAA ATTCCGACAC CATCGAATGG CGCAAAACCT
  301 TTCGCGGTAT GGCATGATAG CGCCCGGAAG AGAGTCAATT CAGGGTGGTG AATGTGAAAC
  361 CAGTAACGTT ATACGATGTC GCAGAGTATG CCGGTGTCTC TTATCAGACC GTTTCCCGCG
  421 TGGTGAACCA GGCCAGCCAC GTTTCTGCGA AAACGCGGGA AAAAGTGGAA GCGGCGATGG
  481 CGGAGCTGAA TTACATTCCC AACCGCGTGG CACAACAACT GGCGGGCAAA CAGTCGTTGC
  541 TGATTGGCGT TGCCACCTCC AGTCTGGCCC TGCACGCGCC GTCGCAAATT GTCGCGGCGA
  601 TTAAATCTCG CGCCGATCAA CTGGGTGCCA GCGTGGTGGT GTCGATGGTA GAACGAAGCG
  661 GCGTCGAAGC CTGTAAAGCG GCGGTGCACA ATCTTCTCGC GCAACGCGTC AGTGGGCTGA
  721 TCATTAACTA TCCGCTGGAT GACCAGGATG CCATTGCTGT GGAAGCTGCC TGCACTAATG
  781 TTCCGGCGTT ATTTCTTGAT GTCTCTGACC AGACACCCAT CAAACAGTAT TATTTTCTCC
  841 CATGAAGACG GGTACGCGAC TGGGGCGTGG GAGCATCTGG TCGCATTGGG TCACCAGCAA
  901 AATCGCGCTG TTAGCGGGGC CCCATTAAGT TCTGGTCTCG GCGCGTCTGC GTCTGGCCTG
  961 GGCTGGCATA AAATATCTCA CTCGCCAATC GAAATTCAGC CGATAGCGGA ACGGGGGAAG
 1021 GGCGACTGGG ATTGCCATGT TCCCGGGTTT CACCAAAACC AATGCCAAAA TGGCTGGAAT
1081 TGAGGCCCAT CGTTTCCAAC TTGGCGAATG CCTTGGGTTG CTCCAAGACG CGA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [link to RRC of NBRP]