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pMCs-FLAG-Suv39h1-4A-IRES-GFP

Retroviral expression vector of mouse SUV39H1, nucleic acid-binding defective mutant (R84A, K87A, K91A, R95A).

Catalog number RDB15884
Resource name pMCs-FLAG-Suv39h1-4A-IRES-GFP
Clone info. Retroviral expression vector of mouse suppressor of variegation 3-9 homolog 1 (SUV39H1), nucleic acid-binding defective mutant (R84A, K87A, K91A, R95A) tagged with N-terminal FLAG, fused with C-terminal IRES-GFP, see Fig. 3A of Shirai, A. et al., Elife, 1; 6. pii: e25317, 2017.
Comment Expression was confirmed by the depositor with Western blot.
Vector backbone pMCs-IRES-GFP (plasmid)
Size of vector backbone 5.8 kb
Selectable markers Amp^r
Gene/insert name mouse Suv39h1 cDNA
Depositor|Developer Shinkai, Yoichi |
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External Database
mouse Suv39h1
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Shirai, A et al. ELIFE. (6), e25317, 2017)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shirai, A et al. ELIFE. (6), e25317, 2017)。
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          RDB15884 pMCs-FLAG-Suv39h1-4A-IRES-GFP Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Original reference

          original Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201.

          2023.04.06

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