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pGEX6P-3-SUV39H1(39-105)-Y67A (#RDB15882)

Expression vector of mouse SUV39H1, chromodomain (residues 39-105), methylated H3K9 binding mutant (Y67A).

Clone info. Expression vector of mouse suppressor of variegation 3-9 homolog 1 (SUV39H1), chromodomain (CD, residues 39-105), methylated H3K9 binding mutant (Y67A) fused with N-terminal GST, tac promoter, see Fig. 1A and Fig. 2A of Shirai, A. et al., Elife, 1; 6. pii: e25317, 2017.
Comment Expression was confirmed by the depositor with CBB staining and Western blot.
Vector backbone pGEX-6P-3 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name mouse Suv39h1 cDNA
Depositor|Developer Shinkai, Yoichi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Shirai, A et al. ELIFE. (6), e25317, 2017)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shirai, A et al. ELIFE. (6), e25317, 2017)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15882 pGEX6P-3-SUV39H1(39-105)-Y67A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX6P-3-SUV39H1(39-105)-Y67A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15882).

Reference section:

Shirai, A., Kawaguchi, T., Shimojo, H., Muramatsu, D., Ishida-Yonetani, M., Nishimura, Y., Kimura, H., Nakayama, J.I., Shinkai, Y., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15882_A8Dgp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX_F2
Sequence file: RDB15882_A8Dga.seq check
>D05294A3-A8Dg__1_pGEX_F2_H02_08_ABI08.ab1
    1 CAATTAGTTT TGGTTTTTAA AAAACGTATT GAAGCTATCC CACAAATTGA TAAGTACTTG
   61 AAATCCAGCA AGTATATAGC ATGGCCTTTG CAGGGCTGGC AAGCCACGTT TGGTGGTGGC
  121 GACCATCCTC CAAAATCGGA TCTGGAAGTT CTGTTCCAGG GGCCCCTGGG ATCCAATCTG
  181 TATGACTTTG AAGTTGAATA CCTGTGTGAT TATAAGAAGA TCCGTGAGCA GGAGTATTAC
  241 CTGGTTAAGT GGCGTGGGGC TCCCGACTCA GAAAACACCT GGGAGCCACG GCAGAATCTA
  301 AAATGTATAC GAGTTCTTAA GCAGTTCCAC AAGGACTTAG AAAGAGAGCT TGTCCGACGA
  361 CACCGCCGGT CAAAGTGAGT CGACTCGAGC GGCCGCATCG TGACTGACTG ACGATCTGCC
  421 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
  481 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
  541 TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG CGATAGCGGA GTGTATAATT
  601 CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA
  661 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT
  721 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC
  781 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC
  841 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA
  901 AAGATGCTGA AGATCAGTTG GGGTGCACGA GTGGGTTACA TCGAACTGGG ATCTCAACAG
  961 CGGTAAGATC CTTGGAGAGT TTTCGCCCCG AAGAAACGTT TTCCAATGAT GAGCACTTTT
 1021 TAAAGGTTCT GCCTATGTGG CGCGGTATTA TCCCGTGATG ACGCCGGGCA AGAGCAACTT
 1081 CGGTCGCGGC ATACACTATT CTCAGAATGA ACTGGGTTTG AGTACCTCCC AGTCACAAGA
1141 AGAGGCACTC TACGGATGGC ATGACAGGTT AAGGAGAGTC A
//
Primer: pGEX-ptac_up-F
Sequence file: RDB15882_A8Dgb.seq check
>D05294A3-1_A8Dg_pGEX-ptac_up-F_E01_05_ABI08.ab1
    1 TTGGAAGGGG ACATCATAAC GGTTCTGGCA ATATTCTGAA ATGAGGCTGT TGACAATTAA
   61 TCATCGGCTC GTATAATGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGT
  121 ATTCATGTCC CCTATACTAG GTTATTGGAA AATTAAGGGC CTTGTGCAAC CCACTCGACT
  181 TCTTTTGGAA TATCTTGAAG AAAAATATGA AGAGCATTTG TATGAGCGCG ATGAAGGTGA
  241 TAAATGGCGA AACAAAAAGT TTGAATTGGG TTTGGAGTTT CCCAATCTTC CTTATTATAT
  301 TGATGGTGAT GTTAAATTAA CACAGTCTAT GGCCATCATA CGTTATATAG CTGACAAGCA
  361 CAACATGTTG GGTGGTTGTC CAAAAGAGCG TGCAGAGATT TCAATGCTTG AAGGAGCGGT
  421 TTTGGATATT AGATACGGTG TTTCGAGAAT TGCATATAGT AAAGACTTTG AAACTCTCAA
  481 AGTTGATTTT CTTAGCAAGC TACCTGAAAT GCTGAAAATG TTCGAAGATC GTTTATGTCA
  541 TAAAACATAT TTAAATGGTG ATCATGTAAC CCATCCTGAC TTCATGTTGT ATGACGCTCT
  601 TGATGTTGTT TTATACATGG ACCCAATGTG CCTGGATGCG TTCCCAAAAT TAGTTTGTTT
  661 TAAAAAACGT ATTGAAGCTA TCCCACAAAT TGATAAGTAC TTGAAATCCA GCAAGTATAT
  721 AGCATGGCCT TTGCAGGGCT GGCAAGCCAC GTTTGGTGGT GGCGACCATC CTCCAAAATC
  781 GGATCTGGAA GTTCTGTTCC AGGGGCCCCT GGGATCCAAT CTGTATGACT TTGAAGTTGA
  841 ATACCTGTGT GATTATAAGA AAGATCCGTG AGCAGGAGTA TTACCTGGTT AAGTGGCGTG
  901 GGGGCTCCCG ACTCAGAAAA CACCTGGGGA GCCACGGGCA GAATCTAAAA ATGTATACGG
  961 AGTTTCTTAA AGCAGTTTCC ACAAAGGGAC TTTAGAAAGG AGAAGCTTTG TTCCGGACGA
 1021 CACCGGCCGG GTCAAAGTGA AGTCGGACTC GAGCGGGCCC GCATCGGTGG ACTGACTGAC
 1081 GATCTGGCCT TCGCGCGATT CGGTGAATGG AACGGTGAAA AACCCTTCTG ACACACAATG
1141 CCAGGCT
//
Primer: SV40pA3' element Reverse
Sequence file: RDB15882_A8Dgc.seq check
>D05294A3-1_A8Dg_SV40pA3'elementReverse_F01_06_ABI08.ab1
    1 TCTTATGGGG GGGGGGGGGG GCTATGGGGA AAACCGCCAG CAACGGCGGG CCTTTTTACG
   61 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC
  121 TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC
  181 CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT
  241 TACGCATCTG TGCGGTATTT CACACCGCAT AAATTCCGAC ACCATCGAAT GGCGCAAAAC
  301 CTTTCGCGGT ATGGCATGAT AGCGCCCGGA AGAGAGTCAA TTCAGGGTGG TGAATGTGAA
  361 ACCAGTAACG TTATACGATG TCGCAGAGTA TGCCGGTGTC TCTTATCAGA CCGTTTCCCG
  421 CGTGGTGAAC CAGGCCAGCC ACGTTTCTGC GAAAACGCGG GAAAAAGTGG AAGCGGCGAT
  481 GGCGGAGCTG AATTACATTC CCAACCGCGT GGCACAACAA CTGGCGGGCA AACAGTCGTT
  541 GCTGATTGGC GTTGCCACCT CCAGTCTGGC CCTGCACGCG CCGTCGCAAA TTGTCGCGGC
  601 GATTAAATCT CGCGCCGATC AACTGGGTGC CAGCGTGGTG GTGTCGATGG TAGAACGAAG
  661 CGGCGTCGAA GCCTGTAAAG CGGCGGTGCA CAATCTTCTC GCGCAACGCG TCAGTGGGCT
  721 GATCATTAAC TATCCGCTGG ATGACCAGGA TGCCATTGCT GTGGAAGCTG CCTGCACTAA
  781 TGTTCCGGCG TTATTTCTTG ATGTCTCTGA CCAGACACCC ATCAACAGTA TTATTTTCTC
  841 CCATGAAGAC GGTACGCGAC TGGGGCGTGG AGCATCTGGG TCGCATTTGG GTCACCCAGC
  901 CAAATTCGCG GCTTGTTTAG CCGGGGCCTC AATTAAAGTT TCTGGTCTCG GGCGCGTTCT
  961 GGCGTTCTGG GCTGGGCTGG GGCATTAAAA TATTCTCAAC TCCGCCAAAT CCAAAATTTT
 1021 CAGGCCCGAA TTAGCCGGGG AACCGGGGAA AGGGGCCGGC CTTGGGAAGT GGCCCATGAT
 1081 TCCCGGGGTT TTTCCAGCAA ACCCCAATTG GCCAAATTGC TGGGAACTGC AAGGGGGGCA
1141 ATTCGATTTT CCTCCCACCA CCTCTTGGCC GCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [link to RRC of NBRP]