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pGEX6P-3-SUV39H1(39-105) (#RDB15880)

Expression vector of mouse SUV39H1, chromodomain (residues 39-105).

Clone info. Expression vector of mouse suppressor of variegation 3-9 homolog 1 (SUV39H1), chromodomain (CD, residues 39-105) fused with N-terminal GST, tac promoter, see Fig. 1A of Shirai, A. et al., Elife, 1; 6. pii: e25317, 2017.
Comment Expression was confirmed by the depositor with CBB staining and Western blot.
Vector backbone pGEX-6P-3 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name mouse Suv39h1 cDNA
Depositor|Developer Shinkai, Yoichi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Shirai, A et al. ELIFE. (6), e25317, 2017)
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shirai, A et al. ELIFE. (6), e25317, 2017)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15880 pGEX6P-3-SUV39H1(39-105) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX6P-3-SUV39H1(39-105) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15880).

Reference section:

Shirai, A., Kawaguchi, T., Shimojo, H., Muramatsu, D., Ishida-Yonetani, M., Nishimura, Y., Kimura, H., Nakayama, J.I., Shinkai, Y., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15880_A8Dgp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX_F2
Sequence file: RDB15880_A8Dga.seq check
>D05294A1-A8Dg__1_pGEX_F2_F02_06_ABI08.ab1
    1 GTCAAATTGG TTTTGGTTTT TAAAAACGTA TTTGAAAGCT ATCCCACAAA TTGATAAAGT
   61 ACTTGAAATC CAGCAAGTAT ATAGCATGGC CTTTGCAGGG CTGGCAAGCC ACGTTTGGTG
  121 GTGGCGACCA TCCTCCAAAA TCGGATCTGG AAGTTCTGTT CCAGGGGCCC CTGGGATCCA
  181 ATCTGTATGA CTTTGAAGTT GAATACCTGT GTGATTATAA GAAGATCCGT GAGCAGGAGT
  241 ATTACCTGGT TAAGTGGCGT GGGTATCCCG ACTCAGAAAA CACCTGGGAG CCACGGCAGA
  301 ATCTAAAATG TATACGAGTT CTTAAGCAGT TCCACAAGGA CTTAGAAAGA GAGCTTGTCC
  361 GACGACACCG CCGGTCAAAG TGAGTCGACT CGAGCGGCCG CATCGTGACT GACTGACGAT
  421 CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC
  481 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC
  541 GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT GACCCAGTCA CGTAGCGATA GCGGAGTGTA
  601 TAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT
  661 AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT
  721 TTGTTTATTT TTCTAAATAC ATTCAAAATA TGTATCCGCT CATGAGACAA ATAACCCTGA
  781 TAAATGCTTC AATAATATTG AAAAAAGGAA GAGTATGAGT ATTCAAACAT TTCCGTGTCG
  841 CCCTTATTCC CTTTTTTGGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AAGAAAACGC
  901 TGGTTGAAAA GTAAAAGATG CTGAAAGATC AAGTTTGGGT GCACGAAGTG GGTTACAATC
  961 GAAACTGGGA TCTCAACAGG CGGTAAGAAT CCCTTTGGAG AGGTTTTCGC CCCCGGAGGA
 1021 ACGGTTTTTC CCAATGAATG AAACACCTTT TTAAAGTTCT TGCTATGTGG GCCGCCGGGT
 1081 ATTATTCCCC GTGTTTGGAC CGCCCGGGCC AAAGAGCCAA CTCGGTCCGC CCCGCCAATA
 1141 CAACTATATT CCTTCAGAAA TTGAACTTGG ATTTGAAGTA CCCTCCACAC CCAGAGTCCA
1201 CCAGGGAAAG AAGCACAATT TCTCTATTTA GCCGGGA
//
Primer: pGEX-ptac_up-F
Sequence file: RDB15880_A8Dgb.seq check
>D05294A1-1_A8Dg_pGEX-ptac_up-F_A01_01_ABI08.ab1
    1 GGAATGGGGC GCATCATTAA CGGGTTCTGG CAAATATTCT GAAATGAGCT GTTGACAATT
   61 AATCATCGGC TCGTATAATG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA
  121 GTATTCATGT CCCCTATACT AGGTTATTGG AAAATTAAGG GCCTTGTGCA ACCCACTCGA
  181 CTTCTTTTGG AATATCTTGA AGAAAAATAT GAAGAGCATT TGTATGAGCG CGATGAAGGT
  241 GATAAATGGC GAAACAAAAA GTTTGAATTG GGTTTGGAGT TTCCCAATCT TCCTTATTAT
  301 ATTGATGGTG ATGTTAAATT AACACAGTCT ATGGCCATCA TACGTTATAT AGCTGACAAG
  361 CACAACATGT TGGGTGGTTG TCCAAAAGAG CGTGCAGAGA TTTCAATGCT TGAAGGAGCG
  421 GTTTTGGATA TTAGATACGG TGTTTCGAGA ATTGCATATA GTAAAGACTT TGAAACTCTC
  481 AAAGTTGATT TTCTTAGCAA GCTACCTGAA ATGCTGAAAA TGTTCGAAGA TCGTTTATGT
  541 CATAAAACAT ATTTAAATGG TGATCATGTA ACCCATCCTG ACTTCATGTT GTATGACGCT
  601 CTTGATGTTG TTTTATACAT GGACCCAATG TGCCTGGATG CGTTCCCAAA ATTAGTTTGT
  661 TTTAAAAAAC GTATTGAAGC TATCCCACAA ATTGATAAGT ACTTGAAATC CAGCAAGTAT
  721 ATAGCATGGC CTTTGCAGGG CTGGCAAGCC ACGTTTGGTG GTGGCGACCA TCCTCCAAAA
  781 TCGGATCTGG AAGTTCTGTT CCAGGGGCCC CTGGGGATCC AATCTGTATG ACTTTGAAGT
  841 TGAATACCTG TGTGATTATA AGAAGATCCG TGAGCAGGAG TATTACCTGG TTAAGTGGCG
  901 TGGGGTATCC CGACTCAGAA AACACCTGGG GAGCCACGGC AGAATCTAAA ATGTATACGG
  961 AGTTTCTTAA GCAGTTCCAC AAGGGACTTT AGAAAGAGAG CTTTGTCCGA CGACACCCGC
 1021 CGGGTCAAAG GTGAGGTCGG ACTTCGAGCG GGCCGCATTC GTGACTGACC TGACGAATTC
 1081 TGCCCTTCGC GCGTTTCCGG TGAATGACGG GTGAAAAAAC CTCTCTTGGA CACACATGTT
1141 GTC
//
Primer: SV40pA3' element Reverse
Sequence file: RDB15880_A8Dgc.seq check
>D05294A1-1_A8Dg_SV40pA3'elementReverse_B01_02_ABI08.ab1
    1 GCGGTTGGGG GGGGGGGGGA GCCTTATGGG AAAACGGCCA GCAACGGCGG GCCTTTTTAC
   61 GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT
  121 CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA
  181 CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC
  241 TTACGCATCT GTGCGGTATT TCACACCGCA TAAATTCCGA CACCATCGAA TGGCGCAAAA
  301 CCTTTCGCGG TATGGCATGA TAGCGCCCGG AAGAGAGTCA ATTCAGGGTG GTGAATGTGA
  361 AACCAGTAAC GTTATACGAT GTCGCAGAGT ATGCCGGTGT CTCTTATCAG ACCGTTTCCC
  421 GCGTGGTGAA CCAGGCCAGC CACGTTTCTG CGAAAACGCG GGAAAAAGTG GAAGCGGCGA
  481 TGGCGGAGCT GAATTACATT CCCAACCGCG TGGCACAACA ACTGGCGGGC AAACAGTCGT
  541 TGCTGATTGG CGTTGCCACC TCCAGTCTGG CCCTGCACGC GCCGTCGCAA ATTGTCGCGG
  601 CGATTAAATC TCGCGCCGAT CAACTGGGTG CCAGCGTGGT GGTGTCGATG GTAGAACGAA
  661 GCGGCGTCGA AGCCTGTAAA AGCGGCGGTG CACAATCTTC TCGCGCAACG CGTCAGTGGG
  721 CTGATCATTA ACTATCCGCT GGATGACCAG GATGCCATTG CTGTGGAAGC TGCCTGCACT
  781 AATGTTCCGG CGTTATTTCT TGATGTCTCT GACCAGACAC CCATCAACAG TATTATTTTT
  841 CTCCCATGAA GACGGTACGC GACTGGGCGT GGAGCATCTG GTCGCATTGG GTTCACCAAG
  901 CAAAATCGCG CTGGTTAGCG GGGCCCATTA AAGTTTCTGT CTCGGCGCGT CTGCGTCCTG
  961 GGCTGGCTGG CATAAAATAT CTCAACTCGC AATCAAAATT TCAAGCCCGA ATAGCGGAAA
 1021 CGGGAAAGGC CGAACTGGAA GTGCCAATGT TCCGGTTTTT CCACAAAACC ATGCAAAAAT
1081 GCTGGATTGG AGGCATCGAT TCCCAACTTG CGATGCTGGC TTGTCCAAAC CGATAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [link to RRC of NBRP]