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Riki Ishibashi's Resource

Riki Ishibashi's web page in English /  in Japanese

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Catalog #Name of cloneRunning titlePublication
RDB20013pCriMGET_9-12aPlasmid vector to construct a donor cassette for knock-in of long external DNA cleaved by both CRISPR-Cas9 and CRISPR-Cas12a.Ishibashi, R., Development of an in vivo cleavable donor plasmid for targeted transgene integration by CRISPR-Cas9 and CRISPR-Cas12a. Sci. Rep. 12 (1): 17775 (2022). PMID 36272994. (original). [link to RRC of NBRP]
RDB19323pX330-AAVS1-sgRNAExpression vector for hCas9 and guideRNA of human AAVS1 locus.Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. (original). [link to RRC of NBRP]
RDB19322pCriMGET_v1-Clec4f-IRES-hDTR-2A-EGFPTargeting construct ot insert IRES-hDTR-P2A-EGFP into 3'UTR region of mouse Clec4f gene.Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. (original). [link to RRC of NBRP]
RDB19321pCriMGET_v1-Tbx3-3xFlag-2A-EGFPTargeting construct to insert 3xFlag-P2A-EGFP into flanked stop codon of mouse Tbx3 gene.Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. (original). [link to RRC of NBRP]
RDB19320pCriMGET_v1-AAVS1-SA-Neo-pATargeting construct to insert Splicing Acceptor (SA)-neomycin resistance gene (neo)-bGHpA into the human AAVS1 locus by the homologous recombination.Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. (original). [link to RRC of NBRP]
RDB19319pX330-syn-crRNA-TS-sgRNAExpression vector of Cas9 and guide RNA in order to excise the targeting cassette cloned in the pCriMGET_v1 vector (RDB19318).Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. (original). [link to RRC of NBRP]
RDB19318pCriMGET_v1Plasmid vector to clone a targeting cassette. By using pX330-syn-crRNA-TS-sgRNA (RDB19319), the targeting cassette can be excised in vivo.Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. (original). [link to RRC of NBRP]

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No Approval Forms Required for Fluorescent Protein Genes Developed by Dr. Atsushi Miyawaki
♦ RIKEN BRC will be closed from December 28, 2024 through January 5, 2025 due to New Year's holidays.
A bicistronic cell cycle reporter, Fucci2a (Sep 17, 2024)
Monomeric Fluorescent Protein Resource, mStayGold (Dec 18, 2023)
Visualization of Organelles update (Dec 18, 2023)
Development of two mouse strains conditionally expressing bright luciferases (Sep 08, 2023)
Autophagy and Mitophagy Updates (Aug 16, 2023)
High intensity forms of luciferase and luminescent proteins from various organisms (BRC RESOURCE NEWS) (Apr 28, 2023)
Plasmid of Cas9 expression/mRNA production, evaluation of the genome edit efficiency, and Knock-in donors and tags
Fucci cell cycle indicator, Calcium sensor and Fluorescent and Luminescent protein resources
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2025.01.22

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