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Riki Ishibashi's web page in English / in Japanese
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Catalog # | Name of clone | Running title | Publication |
RDB20013 | pCriMGET_9-12a | Plasmid vector to construct a donor cassette for knock-in of long external DNA cleaved by both CRISPR-Cas9 and CRISPR-Cas12a. | Ishibashi, R., Development of an in vivo cleavable donor plasmid for targeted transgene integration by CRISPR-Cas9 and CRISPR-Cas12a. Sci. Rep. 12 (1): 17775 (2022). PMID 36272994. (original). [link to RRC of NBRP] |
RDB19323 | pX330-AAVS1-sgRNA | Expression vector for hCas9 and guideRNA of human AAVS1 locus. | Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. (original). [link to RRC of NBRP] |
RDB19322 | pCriMGET_v1-Clec4f-IRES-hDTR-2A-EGFP | Targeting construct ot insert IRES-hDTR-P2A-EGFP into 3'UTR region of mouse Clec4f gene. | Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. (original). [link to RRC of NBRP] |
RDB19321 | pCriMGET_v1-Tbx3-3xFlag-2A-EGFP | Targeting construct to insert 3xFlag-P2A-EGFP into flanked stop codon of mouse Tbx3 gene. | Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. (original). [link to RRC of NBRP] |
RDB19320 | pCriMGET_v1-AAVS1-SA-Neo-pA | Targeting construct to insert Splicing Acceptor (SA)-neomycin resistance gene (neo)-bGHpA into the human AAVS1 locus by the homologous recombination. | Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. (original). [link to RRC of NBRP] |
RDB19319 | pX330-syn-crRNA-TS-sgRNA | Expression vector of Cas9 and guide RNA in order to excise the targeting cassette cloned in the pCriMGET_v1 vector (RDB19318). | Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. (original). [link to RRC of NBRP] |
RDB19318 | pCriMGET_v1 | Plasmid vector to clone a targeting cassette. By using pX330-syn-crRNA-TS-sgRNA (RDB19319), the targeting cassette can be excised in vivo. | Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. (original). [link to RRC of NBRP] |
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♦ RIKEN BRC will be closed from December 28, 2024 through January 5, 2025 due to New Year's holidays.
♦ A bicistronic cell cycle reporter, Fucci2a (Sep 17, 2024)
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♦ Visualization of Organelles update (Dec 18, 2023)
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♦ Autophagy and Mitophagy Updates (Aug 16, 2023)
♦ High intensity forms of luciferase and luminescent proteins from various organisms (BRC RESOURCE NEWS) (Apr 28, 2023)
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2025.01.22
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